Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,018,760	G→A	*476* (TAG→TAA)	rmuC →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,018,760	0	G	A	100.0%	104.4 / NA	34	*476* (TAG→TAA)	rmuC	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/19);  total (15/19)

ACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAGCCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTGGT  >  NC_000913/4018628‑4018890                                                                                                                                     |                                                                                                                                   aCGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCAtt                                                                                                                              >  2:923752/1‑139 (MQ=255)                 gATTTGGCTGAACAGGCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCg                                                                                                              <  1:138576/139‑1 (MQ=255)                                gCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAggg                                                                                               >  2:124820/1‑139 (MQ=255)                                            gATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                   <  2:610146/139‑1 (MQ=255)                                               gAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATc                                                                                <  1:124820/139‑1 (MQ=255)                                                            ttCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGTGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTacac                                                                        >  2:550239/1‑134 (MQ=255)                                                            ttCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTacac                                                                        <  1:550239/134‑1 (MQ=255)                                                               ggtcggtTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTggg                                                                                                                           >  2:546888/1‑80 (MQ=255)                                                               ggtcggtTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTggg                                                                                                                           <  1:546888/80‑1 (MQ=255)                                                                 tcggtTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACa                                                              >  1:326416/1‑139 (MQ=255)                                                                             cagcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                   >  2:918417/1‑106 (MQ=255)                                                                             cagcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                   <  1:918417/106‑1 (MQ=255)                                                                              agcCGAATGATTAAGCTTATCAACGCGATTATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAg                                                 <  2:898139/139‑1 (MQ=255)                                                                               gcCGAATGATGAAGCTTATCAACGCGATGATGCATATAATAAGCAGTCGCGCTCACCCATTGGGAGTAGTTAAGACGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGGGc                                                <  2:411600/139‑1 (MQ=255)                                                                               gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCAtt                                                                                                                              >  1:305468/1‑61 (MQ=255)                                                                               gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCAtt                                                                                                                              <  2:305468/61‑1 (MQ=255)                                                                               gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGc                                                <  1:131687/139‑1 (MQ=255)                                                                                           aGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGAt                                    <  1:923752/139‑1 (MQ=255)                                                                                                    aCGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTa                                                                                                          <  1:323172/60‑1 (MQ=255)                                                                                                    aCGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTa                                                                                                          >  2:323172/1‑60 (MQ=255)                                                                                                       cgATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCa                                               >  2:147981/1‑116 (MQ=255)                                                                                                       cgATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCa                                               <  1:147981/116‑1 (MQ=255)                                                                                                         atgatgAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTcac                      <  1:845000/139‑1 (MQ=255)                                                                                                                      tCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGc                                                >  2:486754/1‑100 (MQ=255)                                                                                                                      tCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGc                                                <  1:486754/100‑1 (MQ=255)                                                                                                                      tCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGa                              >  2:312724/1‑118 (MQ=255)                                                                                                                      tCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGa                              <  1:312724/118‑1 (MQ=255)                                                                                                                         gcagTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGc                                                >  1:676050/1‑97 (MQ=255)                                                                                                                         gcagTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGc                                                <  2:676050/97‑1 (MQ=255)                                                                                                                            gTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTgaga                                     >  1:239653/1‑105 (MQ=255)                                                                                                                            gTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTgaga                                     <  2:239653/105‑1 (MQ=255)                                                                                                                             tCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAAt                                                                                 >  1:619728/1‑60 (MQ=255)                                                                                                                             tCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTGGt  >  1:64118/1‑139 (MQ=255)                                                                                                                             tCGCGCTAACCCATTGGGAGTACTTAAGCCGGGTAGAAATCTAGGGTATCGACGCCCAAt                                                                                 <  2:619728/60‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   ACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAGCCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTGGT  >  NC_000913/4018628‑4018890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑