Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,024,336 G→A *261* (TAG→TAA
L162L (CTC→CTT
tatD →
rfaH ←
quality control of Tat‑exported FeS proteins, Mg‑dependent cytoplasmic DNase
transcription antitermination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,024,3360GA100.0% 230.3 / NA 71*261* (TAG→TAA
L162L (CTC→CTT
tatD
rfaH
quality control of Tat‑exported FeS proteins, Mg‑dependent cytoplasmic DNase
transcription antitermination protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/38);  total (33/38)

GGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCT  >  NC_000913/4024209‑4024450
                                                                                                                               |                                                                                                                  
ggCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGaa                                                                                                         <  2:343893/139‑1 (MQ=255)
                ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                         <  2:737169/139‑1 (MQ=255)
                 cATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                        <  2:493878/139‑1 (MQ=255)
                 cATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                        <  1:39826/139‑1 (MQ=255)
                 cATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                        <  1:441736/139‑1 (MQ=255)
                   tCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   <  1:582974/126‑1 (MQ=255)
                   tCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   >  2:582974/1‑126 (MQ=255)
                   tCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACtg                                                                                      <  1:449017/139‑1 (MQ=255)
                         ccATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTa                                                                                >  2:743747/1‑139 (MQ=255)
                                 tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCttt                                                                        >  1:24135/1‑139 (MQ=255)
                                 tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCttt                                                                        >  2:524019/1‑139 (MQ=255)
                                           tGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGa                                                              >  1:49983/1‑139 (MQ=255)
                                              gcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTa                                                           <  1:471636/139‑1 (MQ=255)
                                                  tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGa                                                                                                          <  1:387536/88‑1 (MQ=255)
                                                  tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGa                                                                                                          >  2:387536/1‑88 (MQ=255)
                                                  tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   >  1:303389/1‑95 (MQ=255)
                                                  tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   <  2:303389/95‑1 (MQ=255)
                                                  tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                       <  1:524019/139‑1 (MQ=255)
                                                     cGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   >  1:9705/1‑92 (MQ=255)
                                                     cGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   <  2:9705/92‑1 (MQ=255)
                                                       tGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                         >  2:492247/1‑100 (MQ=255)
                                                       tGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                         <  1:492247/100‑1 (MQ=255)
                                                              gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   <  1:359639/83‑1 (MQ=255)
                                                              gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   >  2:359639/1‑83 (MQ=255)
                                                              gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   >  2:102687/1‑83 (MQ=255)
                                                              gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   <  1:102687/83‑1 (MQ=255)
                                                              gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                         <  1:570466/93‑1 (MQ=255)
                                                              gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                         >  2:570466/1‑93 (MQ=255)
                                                              gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAAt                                                     >  2:726587/1‑129 (MQ=255)
                                                              gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAAt                                                     <  1:726587/129‑1 (MQ=255)
                                                              gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCa                                                 <  1:741801/133‑1 (MQ=255)
                                                              gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCa                                                 >  2:741801/1‑133 (MQ=255)
                                                              gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgagc                                           >  1:266980/1‑139 (MQ=255)
                                                              gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgagc                                           >  1:818564/1‑139 (MQ=255)
                                                                    gCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCt                                     <  2:708169/139‑1 (MQ=255)
                                                                     cATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGa                                                                                                          <  1:798025/69‑1 (MQ=255)
                                                                     cATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGa                                                                                                          >  2:798025/1‑69 (MQ=255)
                                                                      aTGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGTGGAACTCGGTATTCTTcaca                                                                                         <  1:631896/85‑1 (MQ=255)
                                                                      aTGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                         >  2:631896/1‑85 (MQ=255)
                                                                         gctggctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                        >  2:702361/1‑83 (MQ=255)
                                                                         gctggctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                        <  1:702361/83‑1 (MQ=255)
                                                                             gctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   <  2:656282/68‑1 (MQ=255)
                                                                             gctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   >  1:656282/1‑68 (MQ=255)
                                                                             gctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttct                                                                                              <  2:530827/73‑1 (MQ=255)
                                                                             gctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttct                                                                                              >  1:530827/1‑73 (MQ=255)
                                                                               tgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCg                                                                                                     >  2:384038/1‑64 (MQ=255)
                                                                               tgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCg                                                                                                     <  1:384038/64‑1 (MQ=255)
                                                                                         gATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   >  2:132047/1‑56 (MQ=255)
                                                                                         gATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   <  1:132047/56‑1 (MQ=255)
                                                                                          aTGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAAt               <  2:504684/139‑1 (MQ=255)
                                                                                                      aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   >  2:892192/1‑43 (MQ=255)
                                                                                                      aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                   <  1:892192/43‑1 (MQ=255)
                                                                                                      aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                        <  2:551818/54‑1 (MQ=255)
                                                                                                      aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                        >  2:892805/1‑54 (MQ=255)
                                                                                                      aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                        >  1:551818/1‑54 (MQ=255)
                                                                                                      aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                        <  1:892805/54‑1 (MQ=255)
                                                                                                       aaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                         >  2:328951/1‑52 (MQ=255)
                                                                                                       aaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                         <  1:328951/52‑1 (MQ=255)
                                                                                                       aaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCt  <  2:266980/139‑1 (MQ=255)
                                                                                                          acTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGa                                                              <  2:796865/76‑1 (MQ=255)
                                                                                                          acTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGa                                                              >  1:796865/1‑76 (MQ=255)
                                                                                                             gTTTGGGATTGCGTTTTAAAGTTTGTGGAACTCGGTATTCTTCACACTGTGCTTa                                                                                >  2:137078/1‑55 (MQ=255)
                                                                                                             gTTTGGGATTGCGTTTTAAAGTTTGTGGAACTCGGTATTCTTCACACTGTGCTTa                                                                                <  1:137078/55‑1 (MQ=255)
                                                                                                                tGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                        <  1:562169/44‑1 (MQ=255)
                                                                                                                tGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                        >  2:562169/1‑44 (MQ=255)
                                                                                                                 gggATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                         <  1:606606/42‑1 (MQ=255)
                                                                                                                 gggATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                         >  2:606606/1‑42 (MQ=255)
                                                                                                                        cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTaa                                                                               >  1:822448/1‑45 (MQ=255)
                                                                                                                        cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTaa                                                                               <  2:822448/45‑1 (MQ=255)
                                                                                                                        cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAAt                                                     >  1:373962/1‑71 (MQ=255)
                                                                                                                        cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAAt                                                     <  2:373962/71‑1 (MQ=255)
                                                                                                                               |                                                                                                                  
GGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCT  >  NC_000913/4024209‑4024450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: