Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,290,119	C→T	C104C (TGC→TGT)	nrfC →	formate‑dependent nitrite reductase, 4Fe4S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,290,119	0	C	T	100.0%	124.6 / NA	39	C104C (TGC→TGT)	nrfC	formate‑dependent nitrite reductase, 4Fe4S subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/21);  total (18/21)

ACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGCCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTATCCATCCGGTCACGAAGACGGC  >  NC_000913/4289986‑4290253                                                                                                                                      |                                                                                                                                       aCAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGAc                                                                                                                                   <  2:206067/139‑1 (MQ=255)                gCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTcg                                                                                                                    >  2:10105/1‑139 (MQ=255)                 cGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACTTCTGTCCGACCGGTGCGTCTTTTcgc                                                                                                                   >  2:784140/1‑139 (MQ=255)                             gACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGt                                                                                                       >  2:326008/1‑139 (MQ=255)                                                ccGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGt                                                                                               <  2:838894/128‑1 (MQ=255)                                                ccGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGt                                                                                               >  1:838894/1‑128 (MQ=255)                                                ccGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATc                                                                                    >  2:663974/1‑139 (MQ=255)                                                ccGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATc                                                                                    >  2:733874/1‑139 (MQ=255)                                                  gCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCg                                                                                  <  2:46978/139‑1 (MQ=255)                                                               ccTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     <  1:326008/139‑1 (MQ=255)                                                               ccTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     <  1:733874/139‑1 (MQ=255)                                                                  gATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGtt                                                                  <  2:690336/139‑1 (MQ=255)                                                                    tgtgAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGAtgct                                                                                                             >  2:624565/1‑94 (MQ=255)                                                                    tgtgAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGAtgct                                                                                                             <  1:624565/94‑1 (MQ=255)                                                                     gtgAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTc                                                               >  1:680611/1‑139 (MQ=255)                                                                                  tcttcCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgc                                                  <  1:806040/139‑1 (MQ=255)                                                                                    ttcCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                <  1:380148/139‑1 (MQ=255)                                                                                    ttcCGTAAGTCTTGCCAGCACTGCCATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                <  1:10105/139‑1 (MQ=255)                                                                                                tGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     >  1:801290/1‑106 (MQ=255)                                                                                                tGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     <  2:801290/106‑1 (MQ=255)                                                                                                 gCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACcg                                   >  2:730997/1‑139 (MQ=255)                                                                                                  ccAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGt                                                                                                       >  2:275827/1‑70 (MQ=255)                                                                                                  ccAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGt                                                                                                       <  1:275827/70‑1 (MQ=255)                                                                                                         tGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGtt                                                                  >  1:53194/1‑100 (MQ=255)                                                                                                         tGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGtt                                                                  <  2:53194/100‑1 (MQ=255)                                                                                                                 tGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     <  1:753625/89‑1 (MQ=255)                                                                                                                 tGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     >  2:753625/1‑89 (MQ=255)                                                                                                                  gcgcCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTatccatc                  <  1:140788/139‑1 (MQ=255)                                                                                                                  gcgcCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTatccatc                  <  1:301415/139‑1 (MQ=255)                                                                                                                    gcCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTATCCATCCg                <  2:680611/139‑1 (MQ=255)                                                                                                                        tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCgctgct                                                                                                             <  2:541537/42‑1 (MQ=255)                                                                                                                        tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGAtgct                                                                                                             >  1:541537/1‑42 (MQ=255)                                                                                                                        tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAg                                                             <  1:760868/90‑1 (MQ=255)                                                                                                                        tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAg                                                             >  2:760868/1‑90 (MQ=255)                                                                                                                        tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                >  1:850555/1‑103 (MQ=255)                                                                                                                        tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                <  2:850555/103‑1 (MQ=255)                                                                                                                          cGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTgcgc                            <  2:490609/121‑1 (MQ=255)                                                                                                                          cGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTgcgc                            >  1:490609/1‑121 (MQ=255)                                                                                                                                  tCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTATCCATCCGGTCACGAAGAcggc  >  1:65097/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                       ACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGCCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTATCCATCCGGTCACGAAGACGGC  >  NC_000913/4289986‑4290253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑