Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,424,051 G→A D118N (GAC→AAC)  ulaF → L‑ribulose 5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,424,0510GA100.0% 131.4 / NA 41D118N (GAC→AAC) ulaFL‑ribulose 5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/17);  total (24/17)

CTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAA  >  NC_000913/4423923‑4424183
                                                                                                                                |                                                                                                                                    
cTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTActtctt                                                                                                                            <  2:440343/139‑1 (MQ=255)
                     aCCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCATGCCAACTACTTCTTTGGCGACATTCCGTGTAcgcg                                                                                                       >  2:465330/1‑139 (MQ=255)
                           gtTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGtt                                                                                                 <  2:814839/139‑1 (MQ=255)
                            ttACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTa                                                                                                >  2:120711/1‑139 (MQ=255)
                                   tCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCgaag                                                                                         >  1:319092/1‑139 (MQ=255)
                                     gCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCgaagaa                                                                                       >  1:249647/1‑139 (MQ=255)
                                              cATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAg                                                                              >  2:343275/1‑139 (MQ=255)
                                                   tCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGaagaag                                                                                      >  2:768367/1‑126 (MQ=255)
                                                   tCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGaagaag                                                                                      <  1:768367/126‑1 (MQ=255)
                                                     cATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGt                                                                       <  1:343275/139‑1 (MQ=255)
                                                           cactccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaac                                                                 <  2:711696/139‑1 (MQ=255)
                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCg                                                                                                                       >  1:37306/1‑83 (MQ=255)
                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCg                                                                                                                       <  2:37306/83‑1 (MQ=255)
                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGt                                                                       >  2:841576/1‑131 (MQ=255)
                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGt                                                                       <  1:841576/131‑1 (MQ=255)
                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                               >  2:353967/1‑139 (MQ=255)
                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                               >  2:314085/1‑139 (MQ=255)
                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                               >  1:870794/1‑139 (MQ=255)
                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                               >  1:512408/1‑139 (MQ=255)
                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                               >  1:140060/1‑139 (MQ=255)
                                                                  ctcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAacac                                                          >  1:997/1‑139 (MQ=255)
                                                                  ctcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAacac                                                          >  1:383570/1‑139 (MQ=255)
                                                                  ctcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGGGAGGGGCAGGTTTGAACTGAacac                                                          >  1:462826/1‑139 (MQ=255)
                                                                    cATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCg                                                        >  1:215805/1‑139 (MQ=255)
                                                                       gCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCa                                                     >  1:835583/1‑139 (MQ=255)
                                                                       gCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCa                                                     >  2:616353/1‑139 (MQ=255)
                                                                            cGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTg                                                <  2:383570/139‑1 (MQ=255)
                                                                              cATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGAt                                              <  2:215805/139‑1 (MQ=255)
                                                                              cATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTGAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGAt                                              <  1:383324/139‑1 (MQ=255)
                                                                                 gggCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTAt                                           >  2:404834/1‑139 (MQ=255)
                                                                                        ggCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACg                                    <  2:835583/139‑1 (MQ=255)
                                                                                         gCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg                                                                            <  1:772001/98‑1 (MQ=255)
                                                                                         gCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg                                                                            >  2:772001/1‑98 (MQ=255)
                                                                                             ggCTGGGGAACCCGGTGTCAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTTCGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGACCTGAACACCGGCAAAGTGATTATCGAAACGCTggg                               <  2:318320/139‑1 (MQ=255)
                                                                                             ggCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGc                                                                                                                        <  2:911476/50‑1 (MQ=255)
                                                                                             ggCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGc                                                                                                                        >  1:911476/1‑50 (MQ=255)
                                                                                             ggCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAagag                                                                                    >  2:913490/1‑86 (MQ=255)
                                                                                             ggCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAagag                                                                                    <  1:913490/86‑1 (MQ=255)
                                                                                                       cccGGTGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAg                     >  2:312895/1‑139 (MQ=255)
                                                                                                                  gCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATAc          <  1:353967/139‑1 (MQ=255)
                                                                                                                          cacgcCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGaa  <  1:404834/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                    
CTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAA  >  NC_000913/4423923‑4424183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑