Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,490,375	C→T	A255A (GCG→GCA)	idnR ←	transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,490,375	0	C	T	100.0%	77.0 / NA	25	A255A (GCG→GCA)	idnR	transcriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/15);  total (10/15)

ATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGG  >  NC_000913/4490247‑4490505                                                                                                                                 |                                                                                                                                   atatCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCg                                                                                                                          <  1:356035/139‑1 (MQ=255)   atCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCa                                                                                                                        <  1:707082/139‑1 (MQ=255)            gggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCa                                                                                                               >  2:686432/1‑139 (MQ=255)             ggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCAt                                                                                                              >  1:846199/1‑139 (MQ=255)                           ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                                                                <  2:846199/139‑1 (MQ=255)                            gCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGt                                                                                                          >  2:89380/1‑128 (MQ=255)                            gCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGt                                                                                                          <  1:89380/128‑1 (MQ=255)                                      ggATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATc                                                                                     >  1:328826/1‑139 (MQ=255)                                      ggATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATc                                                                                     >  1:238905/1‑139 (MQ=255)                                           aTTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCAcc                                                                                                                               <  1:807862/92‑1 (MQ=255)                                           aTTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCAcc                                                                                                                               >  2:807862/1‑92 (MQ=255)                                             ttGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGACATTGGTGCAAAATACACCGTCTAAATCCGGGttt                                                                              >  2:239722/1‑139 (MQ=255)                                                    ccAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                                                                <  1:229419/114‑1 (MQ=255)                                                    ccAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                                                                >  2:229419/1‑114 (MQ=255)                                                                  aTGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCAtt                                                         <  1:686432/139‑1 (MQ=255)                                                                   tGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTa                                                        >  1:395211/1‑139 (MQ=255)                                                                     aaaaCCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAAc                                                      <  2:328826/139‑1 (MQ=255)                                                                        aCCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGc                                                   <  2:629022/139‑1 (MQ=255)                                                                              aaTGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCt                                             >  1:124199/1‑139 (MQ=255)                                                                              aaTGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCt                                             <  1:48193/139‑1 (MQ=255)                                                                              aaTGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCt                                             <  1:638594/139‑1 (MQ=255)                                                                                  gAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaat                                         <  2:395211/139‑1 (MQ=255)                                                                                      tttGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaatgaat                                     <  2:238905/139‑1 (MQ=255)                                                                                              ggCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATgaga                             <  2:163947/139‑1 (MQ=255)                                                                                                                         agcagAAGTGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATgggg  <  1:63938/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                   ATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAATGAATGGATGAGATGGCGCGTGGATTCATGCGTAATGGGG  >  NC_000913/4490247‑4490505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑