Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,188	A→G	intergenic (+55/+502)	yjhV → / ← fecE	pseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,188	0	A	G	100.0%	120.0 / NA	35	intergenic (+55/+502)	yjhV/fecE	pseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/18);  total (17/18)

GATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTC  >  NC_000913/4510092‑4510316                                                                                                 |                                                                                                                                 gATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTc                                                                                        <  2:877018/139‑1 (MQ=255)      cATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTc                                                                                   <  1:60095/139‑1 (MQ=255)                  tCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCttt                                                                       <  1:177773/139‑1 (MQ=255)                          tCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGa                                                                                                  <  2:241623/104‑1 (MQ=255)                          tCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGa                                                                                                  >  1:241623/1‑104 (MQ=255)                                 gCCTTTATAAGGTGGCGGCCGTTGATTAAAAAAGCTGCACTTACCTAGGTGGTAGCAACGGAACGCTTTTAGGAAAATTCGATGTTAAGAACCTGG‑TTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGt                                                       <  2:48140/139‑1 (MQ=255)                                           cGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCAtt                                              >  2:254174/1‑139 (MQ=255)                                           cGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATT‑GCAAATTCGTTTCCTGGGTGCTTTTGATTTTCGTTGGCGTcctgaccttg                                             >  1:48140/1‑130 (MQ=255)                                              ggCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAt                                           <  1:131597/139‑1 (MQ=255)                                               gCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGaaa                                                                                                                       <  2:459916/62‑1 (MQ=255)                                               gCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGaaa                                                                                                                       >  1:459916/1‑62 (MQ=255)                                                cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGaaa                                                                                                       >  1:778138/1‑77 (MQ=255)                                                cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGaaa                                                                                                       <  2:778138/77‑1 (MQ=255)                                                cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAttt                                         >  2:255472/1‑139 (MQ=255)                                                cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAttt                                         >  1:200940/1‑139 (MQ=255)                                                               aaaGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCa                          <  1:254174/139‑1 (MQ=255)                                                               aaaGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCa                          <  2:200940/139‑1 (MQ=255)                                                                 aGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAAc                        <  2:268219/139‑1 (MQ=255)                                                                   cTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCt                      >  1:833510/1‑139 (MQ=255)                                                                   cTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCt                      >  2:869165/1‑139 (MQ=255)                                                                              taAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATg           >  1:500819/1‑139 (MQ=255)                                                                                   agtagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATc                                      >  2:353638/1‑107 (MQ=255)                                                                                   agtagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATc                                      <  1:353638/107‑1 (MQ=255)                                                                                   agtagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCa      <  2:560986/139‑1 (MQ=255)                                                                                    gtagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAg     <  2:95427/139‑1 (MQ=255)                                                                                     tagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCa                          <  1:592219/117‑1 (MQ=255)                                                                                     tagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCa                          >  2:592219/1‑117 (MQ=255)                                                                                      agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGcc                            >  1:747325/1‑114 (MQ=255)                                                                                      agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGcc                            <  2:747325/114‑1 (MQ=255)                                                                                       gCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTc  >  2:2904/1‑139 (MQ=255)                                                                                       gCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTc  >  1:282276/1‑139 (MQ=255)                                                                                          aaCGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCaa                                                                                           >  2:168285/1‑47 (MQ=255)                                                                                          aaCGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCaa                                                                                           <  1:168285/47‑1 (MQ=255)                                                                                          aaCGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCt                                                                         >  2:801625/1‑65 (MQ=255)                                                                                          aaCGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCt                                                                         <  1:801625/65‑1 (MQ=255)                                                                                                 |                                                                                                                                 GATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTC  >  NC_000913/4510092‑4510316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑