Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,515,284 C→T G465D (GGC→GAC)  fecA ← TonB‑dependent outer membrane ferric citrate transporter and signal transducer, ferric citrate extracelluar receptor, FecR‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,515,2840CT100.0% 117.5 / NA 37G465D (GGC→GAC) fecATonB‑dependent outer membrane ferric citrate transporter and signal transducer, ferric citrate extracelluar receptor, FecR‑interacting protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/19);  total (18/19)

TGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGG  >  NC_000913/4515160‑4515414
                                                                                                                            |                                                                                                                                  
tGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGc                                                                                                                      >  1:283256/1‑139 (MQ=255)
tGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGc                                                                                                                      <  2:283256/139‑1 (MQ=255)
       atatGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCactgc                                                                                                               >  1:37298/1‑139 (MQ=255)
            ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtg                                                                                                          >  1:351931/1‑139 (MQ=255)
             tCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                         <  1:115153/139‑1 (MQ=255)
                aaaCGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                         >  1:194359/1‑136 (MQ=255)
                aaaCGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                         <  2:194359/136‑1 (MQ=255)
                aaaCGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggt                                                                                                           <  1:775415/134‑1 (MQ=255)
                aaaCGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggt                                                                                                           >  2:775415/1‑134 (MQ=255)
                aaaCGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCgg                                                                                                      <  2:804521/139‑1 (MQ=255)
                        aCCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTaa                                                                                              <  2:322226/139‑1 (MQ=255)
                              cGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                         <  1:635351/122‑1 (MQ=255)
                              cGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                         >  2:635351/1‑122 (MQ=255)
                                       ccAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGtt                                                                               <  2:710821/139‑1 (MQ=255)
                                            tGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCgctgct                                                                                                              <  2:831373/103‑1 (MQ=255)
                                            tGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCgctgct                                                                                                              >  1:831373/1‑103 (MQ=255)
                                                  tGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                         >  2:513125/1‑102 (MQ=255)
                                                  tGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                         <  1:513125/102‑1 (MQ=255)
                                                                           ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCg                                           >  2:806488/1‑139 (MQ=255)
                                                                           ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCg                                           >  1:453803/1‑139 (MQ=255)
                                                                            gCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGt                                          >  2:183580/1‑139 (MQ=255)
                                                                              gTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTgc                                        >  1:551060/1‑139 (MQ=255)
                                                                               tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTgcg                                       >  2:111969/1‑139 (MQ=255)
                                                                               tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTgcg                                       >  1:46801/1‑139 (MQ=255)
                                                                               tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTgcg                                       >  2:84006/1‑139 (MQ=255)
                                                                                      cGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCa                                                                                     >  2:19113/1‑86 (MQ=255)
                                                                                      cGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCa                                                                                     <  1:19113/86‑1 (MQ=255)
                                                                                        gTGCCGGGACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGGCGGCAACTGCCCGCTGCTGGTGGCAGAGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGc                              <  2:260090/139‑1 (MQ=255)
                                                                                        gTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGc                              <  2:429027/139‑1 (MQ=255)
                                                                                        gTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCACTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAggac                              <  2:37298/139‑3 (MQ=255)
                                                                                         tGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGcc                             <  1:72146/139‑1 (MQ=255)
                                                                                          gCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCg                            <  2:72851/139‑1 (MQ=255)
                                                                                               aaCGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCAt                       <  1:183580/139‑1 (MQ=255)
                                                                                                                 aaGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg     <  1:111969/139‑1 (MQ=255)
                                                                                                                   gggCTTGAGTAGGACGGCAACGGGCCGCTGCTGGGGGCGGTGTAGTAACGAATTGCAGGTGTTGATTCATTCAAATAGCGATAGCCCACGCCCACGTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTg   <  2:346318/139‑1 (MQ=255)
                                                                                                                    ggCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTgg  >  2:427071/1‑139 (MQ=255)
                                                                                                                       ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcc                                                        >  1:96026/1‑82 (MQ=255)
                                                                                                                       ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcc                                                        <  2:96026/82‑1 (MQ=255)
                                                                                                                            |                                                                                                                                  
TGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGG  >  NC_000913/4515160‑4515414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑