Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,541,559	G→A	*201* (TAG→TAA)	fimB →	tyrosine recombinase/inversion of on/off regulator of fimA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,541,559	0	G	A	100.0%	185.9 / NA	59	*201* (TAG→TAA)	fimB	tyrosine recombinase/inversion of on/off regulator of fimA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/30);  total (29/30)

CGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAGAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAGTGTGATTCTTAATTATCATAATAAACATTAAGTTAACCA  >  NC_000913/4541431‑4541693                                                                                                                                 |                                                                                                                                       cgACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTga                                                                                                                              <  2:320313/139‑1 (MQ=255)  gACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTgag                                                                                                                             <  1:879099/139‑1 (MQ=255)              aTTATCTTGGGCATCGCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATg                                                                                                                 <  2:720793/139‑1 (MQ=255)                      gggCATCGCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTaaa                                                                                                         >  1:255392/1‑139 (MQ=255)                            cGCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTAc                                                                                                   >  1:911102/1‑139 (MQ=255)                            cGCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTAc                                                                                                   >  2:708322/1‑139 (MQ=255)                             gCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACa                                                                                                  <  1:708322/139‑1 (MQ=255)                             gCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACa                                                                                                  <  1:909201/139‑1 (MQ=255)                              cAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACat                                                                                                 <  2:68096/139‑1 (MQ=255)                                      gTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGa                                                                                                                   <  2:874586/113‑1 (MQ=255)                                      gTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGa                                                                                                                   >  1:874586/1‑113 (MQ=255)                                           aCTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGata                                                                                    >  2:314130/1‑139 (MQ=255)                                               tCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATaaag                                                                                                                                      >  2:810958/1‑85 (MQ=255)                                               tCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATaaag                                                                                                                                      <  1:810958/85‑1 (MQ=255)                                               tCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATc                                                                                >  2:27944/1‑139 (MQ=255)                                               tCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCT‑GGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCa                                                                               >  1:134096/1‑139 (MQ=255)                                                 tGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCaa                                                                              >  1:139362/1‑139 (MQ=255)                                                         cGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATa                                                                                                                                   <  1:730031/78‑1 (MQ=255)                                                         cGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATa                                                                                                                                   >  2:730031/1‑78 (MQ=255)                                                         cGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGa                                                                                                                   <  1:744756/94‑1 (MQ=255)                                                         cGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGa                                                                                                                   >  2:744756/1‑94 (MQ=255)                                                         cGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGtt                                                                                                            <  2:358336/101‑1 (MQ=255)                                                         cGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGtt                                                                                                            >  1:358336/1‑101 (MQ=255)                                                         cGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGaat                                                                      >  2:55998/1‑137 (MQ=255)                                                         cGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGAtt                                                                      >  1:544138/1‑139 (MQ=255)                                                         cGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGAtt                                                                      >  2:514301/1‑139 (MQ=255)                                                         cGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGAtt                                                                      >  1:198967/1‑139 (MQ=255)                                                           ccAGCAATGCAGGGCGTTTTTACGGCATCTGGGCTAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTaa                                                                    >  2:269767/1‑139 (MQ=255)                                                           ccAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTaa                                                                    >  1:145439/1‑139 (MQ=255)                                                           ccAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTaa                                                                    >  1:879027/1‑139 (MQ=255)                                                            cAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAAc                                                                   <  1:31989/139‑1 (MQ=255)                                                                       ggCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCaaa                                                                             >  2:367611/1‑118 (MQ=255)                                                                       ggCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCaaa                                                                             <  1:367611/118‑1 (MQ=255)                                                                       ggCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAAt                                                        <  1:514301/139‑1 (MQ=255)                                                                           tttttACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGAt                                                    <  2:145439/139‑1 (MQ=255)                                                                           tttttACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGAt                                                    <  1:27944/139‑1 (MQ=255)                                                                           tttttACGGCATCTGGAATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGAt                                                    <  2:655808/139‑1 (MQ=255)                                                                            ttttACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGAt                                                                                                                  >  1:715219/1‑76 (MQ=255)                                                                            ttttACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGAt                                                                                                                  <  2:715219/76‑1 (MQ=255)                                                                            ttttACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATg                                                   >  1:56729/1‑139 (MQ=255)                                                                             tttACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGt                                                  >  2:429851/1‑139 (MQ=255)                                                                                 cGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAAt                                                        <  1:550190/129‑1 (MQ=255)                                                                                 cGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACAATTTATTAAt                                                        >  2:550190/1‑129 (MQ=255)                                                                                         gggATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAgtgt                                      <  1:429851/139‑1 (MQ=255)                                                                                                agCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTga                                                                                                                              >  2:686018/1‑44 (MQ=255)                                                                                                agCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTga                                                                                                                              <  1:686018/44‑1 (MQ=255)                                                                                                agCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAAt                                                        <  1:635424/114‑1 (MQ=255)                                                                                                agCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAAt                                                        >  2:635424/1‑114 (MQ=255)                                                                                                agCCAGAGGACGACAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAGTGTGATTCtt                               >  1:532204/1‑139 (MQ=255)                                                                                                    agagGACGACAGCGTCACGCTGTTTTATAAAGATAGTTga                                                                                                                              <  2:353763/40‑1 (MQ=255)                                                                                                    agagGACGACAGCGTCACGCTGTTTTATAAAGATAGTTga                                                                                                                              >  1:353763/1‑40 (MQ=255)                                                                                                         acgacAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAGTGTGATTCTTAATTATCat                      <  2:56729/139‑1 (MQ=255)                                                                                                           gacAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAGTGTGATTCTTAATTATCataa                    <  2:139362/139‑1 (MQ=255)                                                                                                           gacAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAGTGTGATTCTTAATTATCataa                    >  1:446429/1‑139 (MQ=255)                                                                                                            acAGCGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAGTGTGATTCTT‑ATTATGataatt                  <  2:911102/139‑2 (MQ=255)                                                                                                                cGTCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAGTGTGATTCTTAATTATCATAATAAAc               >  2:233287/1‑139 (MQ=255)                                                                                                                  tCACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATGAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAGTGTGATTCTTAATTATCATAATAAACAt             <  2:544138/139‑1 (MQ=255)                                                                                                                   cACGCTGTTTTATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAGTGTGATTCTTAATTATCATAATAAACAtt            <  1:233287/139‑1 (MQ=255)                                                                                                                           tttATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAGTGTGATTCTTAATTATCATAATAAACATTAAGTTAAc    <  2:585462/139‑1 (MQ=255)                                                                                                                           tttATAAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAGTGTGATTCTTAATTATCATAATAAACATTAAGTTAAc    <  2:291171/139‑1 (MQ=255)                                                                                                                             tataAAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAGTGTGATTCTTAATTATCATAATAAACATTAAGTTAACCa  <  1:50085/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       CGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCATCTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAGAGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAATTGATGTTATTGTAAGTGTGATTCTTAATTATCATAATAAACATTAAGTTAACCA  >  NC_000913/4541431‑4541693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑