Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,565,966	C→T	*427* (TAG→TAA)	yjiN ←	zinc‑type alcohol dehydrogenase‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,565,966	0	C	T	100.0%	106.1 / NA	34	*427* (TAG→TAA)	yjiN	zinc‑type alcohol dehydrogenase‑like protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/17);  total (17/17)

CAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCC  >  NC_000913/4565841‑4566099                                                                                                                              |                                                                                                                                      cAAGTGACATGATTTTGCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGTCGa                                                                                                                          <  1:633001/139‑1 (MQ=255)   aGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTTCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTTGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAgg                                                                                                                        <  2:7983/139‑1 (MQ=255)                      cccGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCt                                                                                                     >  1:471497/1‑139 (MQ=255)                              aCGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                             <  2:471497/139‑1 (MQ=255)                                  gagaTAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAat                                                                                         >  1:856888/1‑139 (MQ=255)                                         tGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAAt                                                                                  <  2:856888/139‑1 (MQ=255)                                                 ggCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCaa                                                                          >  2:275950/1‑139 (MQ=255)                                                   ccACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAgg                                                                                                                        >  1:296830/1‑91 (MQ=255)                                                   ccACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAgg                                                                                                                        <  2:296830/91‑1 (MQ=255)                                                    cacaTTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                                       <  1:275950/139‑1 (MQ=255)                                                                 ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                             <  1:624708/104‑1 (MQ=255)                                                                 ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                             >  2:624708/1‑104 (MQ=255)                                                                 ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAg                                                          >  1:757175/1‑139 (MQ=255)                                                                            tGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGa                                               <  1:104431/139‑1 (MQ=255)                                                                              gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGcc                                                                >  1:775873/1‑120 (MQ=255)                                                                              gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGcc                                                                <  2:775873/120‑1 (MQ=255)                                                                                        tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                                       <  1:658981/103‑1 (MQ=255)                                                                                        tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                                       >  2:658981/1‑103 (MQ=255)                                                                                        tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACt                                   >  1:868302/1‑139 (MQ=255)                                                                                         ctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                            <  1:707710/81‑1 (MQ=255)                                                                                         ctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                            >  2:707710/1‑81 (MQ=255)                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAAt                                                                                  >  2:289210/1‑78 (MQ=255)                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAAt                                                                                  <  1:289210/78‑1 (MQ=255)                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATTCAACCGCCAACCAGCGTACCGTTGACACGAATa                                       >  2:621032/1‑121 (MQ=255)                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                                       <  2:488838/89‑1 (MQ=255)                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                                       >  1:488838/1‑89 (MQ=255)                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGa                                               >  1:836722/1‑113 (MQ=255)                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGa                                               <  2:836722/113‑1 (MQ=255)                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                                       <  1:621032/121‑1 (MQ=255)                                                                                                      aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg                     >  1:425021/1‑139 (MQ=255)                                                                                                       gCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGa                    >  1:95606/1‑139 (MQ=255)                                                                                                           cTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATAtt                <  2:868302/139‑1 (MQ=255)                                                                                                                         gATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGcc  <  1:467497/139‑1 (MQ=255)                                                                                                                         gATTTTTAAAAATTGCAGAGGGGGAACAAGGACGGGAGCTGAGACAGCAACTATAAAATTAGCCCAATACAACCGCGAAGCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGACGATGAACTCTAttgtcc  >  2:666985/1‑135 (MQ=255)                                                                                                                              |                                                                                                                                      CAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCC  >  NC_000913/4565841‑4566099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑