Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,610,462 C→T A350V (GCG→GTG)  prfC → peptide chain release factor RF‑3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,610,4620CT100.0% 84.6 / NA 27A350V (GCG→GTG) prfCpeptide chain release factor RF‑3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)

ACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACT  >  NC_000913/4610345‑4610590
                                                                                                                     |                                                                                                                                
accgcgaccgcgTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTga                                                                                                             <  1:367975/139‑1 (MQ=255)
                         tgtgGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACg                                                                                                                 <  1:200817/110‑1 (MQ=255)
                         tgtgGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACg                                                                                                                 >  2:418783/1‑110 (MQ=255)
                         tgtgGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACg                                                                                                                 >  2:200817/1‑110 (MQ=255)
                         tgtgGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACg                                                                                                                 <  1:418783/110‑1 (MQ=255)
                         tgtgGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTc                                                                                    >  1:62838/1‑139 (MQ=255)
                                       aaTATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCa                                                                      <  2:497113/139‑1 (MQ=255)
                                           tGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGcac                                                                           <  1:185016/130‑1 (MQ=255)
                                           tGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGcac                                                                           >  2:185016/1‑130 (MQ=255)
                                              aaaaGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCAcc                                                               >  1:356201/1‑139 (MQ=255)
                                                            tGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                 <  2:356201/139‑1 (MQ=255)
                                                              cgcCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGaca                                               >  1:468955/1‑139 (MQ=255)
                                                                      gcgcACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTAcc                                       <  2:468955/139‑1 (MQ=255)
                                                                             gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCg                                                                                            >  2:607757/1‑79 (MQ=255)
                                                                             gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCg                                                                                            <  1:607757/79‑1 (MQ=255)
                                                                             gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTg                                <  2:330076/139‑1 (MQ=255)
                                                                              cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                 <  2:312516/121‑1 (MQ=255)
                                                                              cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                 >  1:312516/1‑121 (MQ=255)
                                                                              cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTga                               >  2:394302/1‑139 (MQ=255)
                                                                                 aaGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACAGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAgat                            >  2:658646/1‑139 (MQ=255)
                                                                                                      cgcTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTcc       <  1:443213/139‑1 (MQ=255)
                                                                                                        cTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCt                                            >  2:361442/1‑100 (MQ=255)
                                                                                                        cTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCt                                            <  1:361442/100‑1 (MQ=255)
                                                                                                         tGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGaa    >  1:387899/1‑139 (MQ=255)
                                                                                                           aCCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATc                                                                                                  >  2:80043/1‑43 (MQ=255)
                                                                                                           aCCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATc                                                                                                  <  1:80043/43‑1 (MQ=255)
                                                                                                           aCCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACt  >  1:564776/1‑139 (MQ=255)
                                                                                                                     |                                                                                                                                
ACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACT  >  NC_000913/4610345‑4610590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: