Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,612,289	G→A	*54* (TAG→TAA)	ytjA →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,612,289	0	G	A	100.0%	42.7 / NA	15	*54* (TAG→TAA)	ytjA	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/8);  total (7/8)

TATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGG  >  NC_000913/4612157‑4612401                                                                                                                                     |                                                                                                                 tATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCa                                                                                                            <  1:356645/139‑1 (MQ=255)  aTCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAg                                                                                                           >  1:761362/1‑139 (MQ=255)                                               aCCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCtt                                                              >  2:754721/1‑139 (MQ=255)                                                  gCTGCATGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTTATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAAGTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTg                                                           <  1:888648/139‑1 (MQ=255)                                                   cTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGc                                                          >  2:142781/1‑139 (MQ=255)                                                            cAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGc                                                 <  1:659290/139‑1 (MQ=255)                                                              gCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGa                                                                                                          >  1:901808/1‑80 (MQ=255)                                                              gCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGa                                                                                                          <  2:901808/80‑1 (MQ=255)                                                                     ttGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAAtaataa                                        <  1:754721/139‑1 (MQ=255)                                                                                                     gTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGa                                                                                                 <  1:732645/50‑1 (MQ=255)                                                                                                     gTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGa                                                                                                 >  2:732645/1‑50 (MQ=255)                                                                                                                   gggCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCTAAAGCCAGTCCAGCGGACTGGCtttt                                                            <  1:286922/73‑1 (MQ=255)                                                                                                                   gggCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCTAAAGCCAGTCCAGCGGACTGGCtttt                                                            >  2:286922/1‑73 (MQ=255)                                                                                                                               aCCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAgg  >  1:933043/1‑119 (MQ=255)                                                                                                                               aCCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAgg  <  2:933043/119‑1 (MQ=255)                                                                                                                                     |                                                                                                                 TATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGG  >  NC_000913/4612157‑4612401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑