Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 390,693 A→G pseudogene (443/1458 nt) yaiT → pseudogene, autotransporter family,putative structure, Not classified, interrupted by IS3, putative flagellin structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913390,6930AG99.1% 384.2 / ‑5.1 106pseudogene (443/1458 nt)yaiTpseudogene, autotransporter family,putative structure, Not classified, interrupted by IS3, putative flagellin structural protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (45/60);  total (45/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 106 total aligned reads displayed.

CAGCTTGATATCAGTAATTCAGTGATTCACGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACACTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAACCAAGCACACAAATGAGACATTTGATAC  >  NC_000913/390557‑390825
                                                                                                                                        |                                                                                                                                    
cAGCTTGATATCAGTAATTCAGTGATTCACGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCt                                                                                                                                    >  2:854188/1‑139 (MQ=255)
   cTTGATATCAGTAATTCAGTGATTCACGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGc                                                                                                                                 >  2:65266/1‑139 (MQ=255)
   cTTGATATCAGTAATTCAGTGATTCACGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGc                                                                                                                                 >  2:441446/1‑139 (MQ=255)
        tatCAGTAATTCAGTGATTCACGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCTGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCgtgat                                                                                                                            >  2:430690/1‑139 (MQ=255)
             gTAATTCAGTGATTCACGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCgtgatggtga                                                                                                                       <  2:733637/139‑1 (MQ=255)
                   cAGTGATTCACGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGtttt                                                                                                                 <  2:374264/139‑1 (MQ=255)
                     gTGATTCACGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCg                                                                                                                                >  1:366887/1‑122 (MQ=255)
                     gTGATTCACGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCg                                                                                                                                <  2:366887/122‑1 (MQ=255)
                      tGATTCACGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCAc                                                                                                              >  2:722039/1‑139 (MQ=255)
                           cACGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGa                                                                                                         <  2:119688/139‑1 (MQ=255)
                           cACGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGa                                                                                                         >  1:1193/1‑139 (MQ=255)
                             cGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAAc                                                                                                       <  1:722039/139‑1 (MQ=255)
                              gtttCGACTACTTCTGTGCTGTCAGCCTGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTTTGTGTATATGATTTTTACACTGATCCGGTTGTTGATACACGCTGGCTTGATGGTGATGTTTTCACTTTGAACa                                                                                                      <  2:106870/137‑1 (MQ=255)
                              ggTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACa                                                                                                      <  1:758985/139‑1 (MQ=255)
                                  cGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGc                                                                                                  >  1:689452/1‑139 (MQ=255)
                                   gATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCt                                                                                                 <  2:1193/139‑1 (MQ=255)
                                   gATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCt                                                                                                 <  1:926939/139‑1 (MQ=255)
                                   gATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCt                                                                                                 <  1:65266/139‑1 (MQ=255)
                                   gATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCt                                                                                                 <  1:486901/139‑1 (MQ=255)
                                       aCTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAaca                                                                                             <  2:91069/139‑1 (MQ=255)
                                           cTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACactac                                                                                         <  2:527453/139‑1 (MQ=255)
                                                     tGGCGGTTATTATGATCTTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATtgatgat                                                                               <  1:333776/139‑1 (MQ=255)
                                                          tttattaGGATCGTTGTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTa                                                                          <  2:468622/138‑1 (MQ=255)
                                                            tattatGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATg                                                                        <  1:293240/139‑1 (MQ=255)
                                                                   aTCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTGACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGctct                                                                 >  1:554602/1‑139 (MQ=255)
                                                                   aTCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGaa                                                                                                        <  1:110521/100‑1 (MQ=255)
                                                                   aTCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGaa                                                                                                        >  2:110521/1‑100 (MQ=255)
                                                                       ttttGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTaa                                                                                               >  1:201833/1‑105 (MQ=255)
                                                                       ttttGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTaa                                                                                               <  2:201833/105‑1 (MQ=255)
                                                                       ttttGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTAc                                                             <  2:234970/139‑1 (MQ=255)
                                                                        tttGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAacac                                                                                            <  1:241121/107‑1 (MQ=255)
                                                                        tttGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAacac                                                                                            >  2:241121/1‑107 (MQ=255)
                                                                         ttGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAAc                                                                                                       <  2:845246/95‑1 (MQ=255)
                                                                         ttGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAAc                                                                                                       >  1:845246/1‑95 (MQ=255)
                                                                                  aTGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCt                                                                                                 >  2:826431/1‑92 (MQ=255)
                                                                                  aTGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCt                                                                                                 <  1:826431/92‑1 (MQ=255)
                                                                                  aTGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGAt                                                    <  1:609787/137‑1 (MQ=255)
                                                                                  aTGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGAt                                                    >  2:609787/1‑137 (MQ=255)
                                                                                  aTGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTc                                                  >  1:587042/1‑139 (MQ=255)
                                                                                    ggTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCtt                                                >  2:206162/1‑139 (MQ=255)
                                                                                       aataatCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGaa                                                                                                        <  1:892422/80‑1 (MQ=255)
                                                                                       aataatCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGaa                                                                                                        >  2:892422/1‑80 (MQ=255)
                                                                                          aatCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAg                                          <  2:587042/139‑1 (MQ=255)
                                                                                            tCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATtgat                                                                                  <  1:853842/97‑1 (MQ=255)
                                                                                            tCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATtgat                                                                                  >  2:853842/1‑97 (MQ=255)
                                                                                             cTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATg                                       <  2:771653/139‑1 (MQ=255)
                                                                                              tGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTaa                                                                                               >  1:213825/1‑82 (MQ=255)
                                                                                              tGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTaa                                                                                               <  2:213825/82‑1 (MQ=255)
                                                                                              tGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATgg                                      >  1:309674/1‑139 (MQ=255)
                                                                                              tGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATgg                                      <  1:233268/139‑1 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCt                                                                                                 <  2:563344/78‑1 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCt                                                                                                 >  1:563344/1‑78 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAaca                                                                                             <  2:59309/82‑1 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAaca                                                                                             >  1:59309/1‑82 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAac                                                                                              <  1:907773/81‑1 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAac                                                                                              >  2:907773/1‑81 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGa                                                     <  2:900263/122‑1 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGa                                                     >  2:256196/1‑122 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGa                                                     >  2:842183/1‑122 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGa                                                     >  1:900263/1‑122 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGa                                                     <  1:256196/122‑1 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGa                                                     <  1:842183/122‑1 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGAt                                        >  1:88748/1‑135 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGAt                                        <  2:88748/135‑1 (MQ=255)
                                                                                                ggTGGATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTg                                    >  1:37134/1‑139 (MQ=255)
                                                                                                    gATATGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGt                                >  2:663553/1‑139 (MQ=255)
                                                                                                     atatGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTa                               >  2:412816/1‑139 (MQ=255)
                                                                                                       atGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGaa                                                                                                        <  2:885933/64‑1 (MQ=255)
                                                                                                       atGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGaa                                                                                                        >  1:885933/1‑64 (MQ=255)
                                                                                                        tGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAg                                                                     >  2:21443/1‑98 (MQ=255)
                                                                                                        tGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAg                                                                     <  1:21443/98‑1 (MQ=255)
                                                                                                        tGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAAcc                            <  2:213061/139‑1 (MQ=255)
                                                                                                        tGATTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAAcc                            <  1:196454/139‑1 (MQ=255)
                                                                                                          aTTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACa                                                                                                      <  1:876946/63‑1 (MQ=255)
                                                                                                          aTTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACa                                                                                                      >  2:876946/1‑63 (MQ=255)
                                                                                                          aTTTTTACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAACCaa                          <  1:412816/139‑1 (MQ=255)
                                                                                                             tttACACTGATGCGGTTGTTGATACACACTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAACCAAGca                       <  1:743548/139‑1 (MQ=255)
                                                                                                              ttACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGa                                   >  1:42099/1‑126 (MQ=255)
                                                                                                              ttACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGa                                   <  2:42099/126‑1 (MQ=255)
                                                                                                              ttACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAACCAAGcac                      >  1:249830/1‑139 (MQ=255)
                                                                                                              ttACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATCGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAACCAAGcac                      <  1:36901/139‑1 (MQ=255)
                                                                                                               tACACTGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAACCAAGcaca                     <  1:854188/139‑1 (MQ=255)
                                                                                                                    tGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAacac                                                                                            >  1:464065/1‑63 (MQ=255)
                                                                                                                    tGATGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAacac                                                                                            <  2:464065/63‑1 (MQ=255)
                                                                                                                      aTGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATg                                                                        >  1:61116/1‑81 (MQ=255)
                                                                                                                      aTGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATg                                                                        <  2:61116/81‑1 (MQ=255)
                                                                                                                      aTGCGGTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAACCAAGCACACAAATga              <  2:249830/139‑1 (MQ=255)
                                                                                                                          ggTTGTTGATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAACCAAGCACACAAATGAGACa          <  1:441446/139‑1 (MQ=255)
                                                                                                                                tgATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTaa                                                                                               >  2:804956/1‑48 (MQ=255)
                                                                                                                                tgATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTaa                                                                                               <  1:804956/48‑1 (MQ=255)
                                                                                                                                tgATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATtgat                                                                                  <  1:465858/61‑1 (MQ=255)
                                                                                                                                tgATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATtgat                                                                                  >  2:465858/1‑61 (MQ=255)
                                                                                                                                tgATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACt                                                       >  1:113223/1‑88 (MQ=255)
                                                                                                                                tgATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACt                                                       <  2:113223/88‑1 (MQ=255)
                                                                                                                                tgATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAACCAAGCACACAAATgag             <  1:209507/130‑1 (MQ=255)
                                                                                                                                tgATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAACCAAGCACACAAATgag             >  2:209507/1‑130 (MQ=255)
                                                                                                                                 gATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCt                                                                                                 <  1:264613/45‑1 (MQ=255)
                                                                                                                                 gATACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCt                                                                                                 >  2:264613/1‑45 (MQ=255)
                                                                                                                                  aTACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAACCAAGCACACAAATGAGACATTTGATAc  <  1:206162/139‑1 (MQ=255)
                                                                                                                                  aTACACGCTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAACCAAGCACACAAATGAGACATTTGATAc  <  1:738137/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
CAGCTTGATATCAGTAATTCAGTGATTCACGGTTCGATTACTTCTATGCTGCCTGGCGGTTATTATGATCGTTTTGATGCAGATGGTAATAATCTGGGTGGATATGATTTTTACACTGATGCGGTTGTTGATACACACTGGCGTGATGGTGATGTTTTCACTTTGAACATTGCTAACACTACTATTGATGATGATTATGAAGCTCTTTACTTCACTGATTCTTATAAAGATGGTGATGTAACCAAGCACACAAATGAGACATTTGATAC  >  NC_000913/390557‑390825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: