Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 443,604 C→T S13N (AGT→AAT) 
*304* (TAG→TAA
yajL ←
panE ←
oxidative‑stress‑resistance chaperone
2‑dehydropantoate reductase, NADPH‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913443,6040CT100.0% 88.7 / NA 29S13N (AGT→AAT) 
*304* (TAG→TAA
yajL
panE
oxidative‑stress‑resistance chaperone
2‑dehydropantoate reductase, NADPH‑specific
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/16);  total (13/16)

GCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAA  >  NC_000913/443470‑443729
                                                                                                                                      |                                                                                                                             
gcgcATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTAcc                                                                                                                           <  1:252087/139‑1 (MQ=255)
          cagcagCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAgg                                                                                                                 <  1:375572/139‑1 (MQ=255)
                          cgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGTTACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                                 <  1:744658/139‑1 (MQ=255)
                          cgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                                 <  2:303881/139‑1 (MQ=255)
                                  ggTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCata                                                                                         >  1:768875/1‑139 (MQ=255)
                                                        cTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTAcc                                                                   >  2:757961/1‑139 (MQ=255)
                                                               cGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCaa                                                            <  1:757961/139‑1 (MQ=255)
                                                                          gtgACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGgtg                                                 <  1:566367/139‑1 (MQ=255)
                                                                           tgACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGc                                                                                                                >  2:401630/1‑75 (MQ=255)
                                                                           tgACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGc                                                                                                                <  1:401630/75‑1 (MQ=255)
                                                                                  gATACCGCCGCGAACCAGCAGATCGATAGTGGTGCCGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCgg                                         <  2:807658/139‑1 (MQ=255)
                                                                                       cgccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTAcc                                                                                                                           <  1:74428/52‑1 (MQ=255)
                                                                                       cgccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTAcc                                                                                                                           >  2:74428/1‑52 (MQ=255)
                                                                                         ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                                 <  2:427488/76‑1 (MQ=255)
                                                                                         ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                                 >  1:427488/1‑76 (MQ=255)
                                                                                         ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCa                                  >  2:205409/1‑139 (MQ=255)
                                                                                                          gATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggc                 >  1:736239/1‑139 (MQ=255)
                                                                                                           aTAGTGGTGACGGCTGCTGTCTCTTCATTACCAGGGGCGATGCAAACCAGTGCCGATGCGCGCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggcg                <  1:572620/139‑1 (MQ=255)
                                                                                                              gtggtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                                 <  1:738482/55‑1 (MQ=255)
                                                                                                              gtggtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                                 >  2:738482/1‑55 (MQ=255)
                                                                                                                ggtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCgcggcg           <  1:205409/139‑1 (MQ=255)
                                                                                                                 gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGc                                                      >  2:208635/1‑95 (MQ=255)
                                                                                                                 gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGc                                                      <  1:208635/95‑1 (MQ=255)
                                                                                                                 gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcggg                  >  2:182076/1‑131 (MQ=255)
                                                                                                                 gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcggg                  <  1:182076/131‑1 (MQ=255)
                                                                                                                 gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGt          >  1:610057/1‑139 (MQ=255)
                                                                                                                    aCGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATgcg                                                                                               <  2:467893/51‑1 (MQ=255)
                                                                                                                    aCGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATgcg                                                                                               >  1:467893/1‑51 (MQ=255)
                                                                                                                         ttCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGaaa  >  2:370221/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                             
GCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAA  >  NC_000913/443470‑443729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: