Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,416,763	2 bp→TC	coding (2017‑2018/2145 nt)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,416,763	0	C	T	100.0%	140.5 / NA	44	L673S (CTG→TCG)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/23);  total (21/23)
*	NC_000913	2,416,764	0	T	C	100.0%	156.9 / NA	44	L673S (CTG→TCG)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/23);  total (21/23)

CGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAG  >  NC_000913/2416635‑2416888                                                                                                                                 ||                                                                                                                             cGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGAGTCGATCTCCAt                                                                                                                     >  1:603461/1‑139 (MQ=255)        ggCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCg                                                                                                              <  2:625887/139‑1 (MQ=255)         gCTGACGTTGCGAAGTCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACACAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGa                                                                                                             <  2:933582/139‑1 (MQ=255)                ttGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCa                                                                                                      >  2:695681/1‑139 (MQ=255)                               cgcCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGcc                                                                                       >  1:588091/1‑139 (MQ=255)                                    aaCTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTa                                                                                  <  2:390553/139‑1 (MQ=255)                                         tcCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGAc                                                                             <  2:86928/139‑1 (MQ=255)                                                  aGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGt                                                                    <  1:695681/139‑1 (MQ=255)                                                       gcgcTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGgcgc                                                               >  2:430005/1‑139 (MQ=255)                                                        cgcTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCa                                                              <  2:603461/139‑1 (MQ=255)                                                           tACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTg                                                           >  1:395198/1‑139 (MQ=255)                                                                     aTCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGAtgctg                                                                                                        >  2:923595/1‑84 (MQ=255)                                                                     aTCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGAtgctg                                                                                                        <  1:923595/84‑1 (MQ=255)                                                                     aTCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGata                                                 <  2:395198/139‑1 (MQ=255)                                                                     aTCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGata                                                 <  1:661105/139‑1 (MQ=255)                                                                       cTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATc                                               >  1:569189/1‑139 (MQ=255)                                                                                 cTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGata                                                 <  2:50483/127‑1 (MQ=255)                                                                                 cTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGata                                                 >  1:50483/1‑127 (MQ=255)                                                                                    aaCACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATcg                                  >  2:89034/1‑139 (MQ=255)                                                                                        ccGGTAACACCACCGACTAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCGGATGCTGCAGGGTATGTGCAAGCCGGTTAACGACCTGTGCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCt                              <  2:24881/139‑1 (MQ=255)                                                                                         cGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCg                                                                                                              >  2:350596/1‑58 (MQ=255)                                                                                         cGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCg                                                                                                              <  1:350596/58‑1 (MQ=255)                                                                                         cGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGcc                                                                                       <  1:253114/81‑1 (MQ=255)                                                                                         cGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGcc                                                                                       >  2:253114/1‑81 (MQ=255)                                                                                         cGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGAc                                                                             <  1:522042/91‑1 (MQ=255)                                                                                         cGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGAc                                                                             >  2:522042/1‑91 (MQ=255)                                                                                         cGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTg                             >  1:755864/1‑139 (MQ=255)                                                                                              acacCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGc                        >  2:424732/1‑139 (MQ=255)                                                                                                 ccaccTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGata                                                 >  1:21956/1‑111 (MQ=255)                                                                                                 ccaccTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGata                                                 <  2:21956/111‑1 (MQ=255)                                                                                                      tACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGt                <  2:569189/139‑1 (MQ=255)                                                                                                         aaaGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGat                                                  >  2:489812/1‑102 (MQ=255)                                                                                                         aaaGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGat                                                  <  1:489812/102‑1 (MQ=255)                                                                                                         aaaGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTg             <  2:755864/139‑1 (MQ=255)                                                                                                               gTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGAtgctg                                                                                                        >  1:386701/1‑42 (MQ=255)                                                                                                               gTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGAtgctg                                                                                                        <  2:386701/42‑1 (MQ=255)                                                                                                                  cAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGcc                                                                                       <  1:838796/56‑1 (MQ=255)                                                                                                                  cAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGcc                                                                                       >  2:135848/1‑56 (MQ=255)                                                                                                                  cAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGcc                                                                                       >  2:838796/1‑56 (MQ=255)                                                                                                                  cAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGcc                                                                                       <  1:135848/56‑1 (MQ=255)                                                                                                                   aGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCAcagcagca   >  2:76863/1‑139 (MQ=255)                                                                                                                    gCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCAcagcagcag  <  2:588091/139‑1 (MQ=255)                                                                                                                       ttCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGa                                                      >  1:365035/1‑84 (MQ=255)                                                                                                                       ttCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGa                                                      <  2:365035/84‑1 (MQ=255)                                                                                                                                 ||                                                                                                                             CGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAG  >  NC_000913/2416635‑2416888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑