Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,763,987 Δ1 bp coding (23/1488 nt) sufB ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,763,9870G.100.0% 213.4 / NA 51coding (23/1488 nt)sufBcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (25/26);  total (25/26)

GCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCC  >  NC_000913/1763853‑1764117
                                                                                                                                      |                                                                                                                                  
gCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCt                                                                                                                               >  1:45212/1‑139 (MQ=255)
         gCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTa                                                                                                                      <  1:496971/139‑1 (MQ=255)
                  aaaTTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATa                                                                                                             >  1:51490/1‑139 (MQ=255)
                    aTTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGt                                                                                                           >  2:825101/1‑139 (MQ=255)
                     ttGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTa                                                                                                          <  2:45212/139‑1 (MQ=255)
                               caccTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                                    <  2:1475803/119‑1 (MQ=255)
                               caccTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                                    >  1:1475803/1‑119 (MQ=255)
                                  ctctTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCtt                                                                                                                              <  1:779984/106‑1 (MQ=255)
                                  ctctTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCtt                                                                                                                              >  2:779984/1‑106 (MQ=255)
                                       cGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACgag                                                                                                                  >  2:849269/1‑113 (MQ=255)
                                       cGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACgag                                                                                                                  <  1:849269/113‑1 (MQ=255)
                                             tCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgc                                                                                  <  1:825101/139‑1 (MQ=255)
                                                               cGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                <  2:26044/139‑1 (MQ=255)
                                                                            ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGtt                                                   >  2:1364819/1‑139 (MQ=255)
                                                                              tGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                            >  2:1399854/1‑112 (MQ=255)
                                                                              tGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                            <  1:1399854/112‑1 (MQ=255)
                                                                                        ttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGAc                                                                                                                >  2:1373365/1‑66 (MQ=255)
                                                                                        ttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGAc                                                                                                                <  1:1373365/66‑1 (MQ=255)
                                                                                          cttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAg                                                                                                                           >  2:1401329/1‑53 (MQ=255)
                                                                                          cttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAg                                                                                                                           <  1:1401329/53‑1 (MQ=255)
                                                                                               taATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTc                                                                                                                             <  1:1285003/46‑1 (MQ=255)
                                                                                               taATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTc                                                                                                                             >  2:1285003/1‑46 (MQ=255)
                                                                                               taATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcatt                                                                      >  1:1350999/1‑101 (MQ=255)
                                                                                               taATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcatt                                                                      <  2:1350999/101‑1 (MQ=255)
                                                                                                aaTTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTc                                                                                                                             >  2:263767/1‑45 (MQ=255)
                                                                                                aaTTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTc                                                                                                                             <  1:263767/45‑1 (MQ=255)
                                                                                                     aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCACTTATACCCCAAAGCTTTCGCCACAGCCAcat                                                                       >  1:541644/1‑94 (MQ=255)
                                                                                                     aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCACTTATACCCCAAAGCTTTCGCCACAGCCAcat                                                                       <  2:541644/94‑1 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                                    >  2:844610/1‑47 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                                    <  1:844610/47‑1 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACa                                                                                                               >  1:1372811/1‑52 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACa                                                                                                               <  2:1372811/52‑1 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcc                                                                                 >  1:1235776/1‑82 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcc                                                                                 <  2:1235776/82‑1 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAatat                                    <  1:1327057/127‑1 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAatat                                    >  2:1327057/1‑127 (MQ=255)
                                                                                                            cgccgGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                                    >  1:387487/1‑42 (MQ=255)
                                                                                                            cgccgGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                                    <  2:387487/42‑1 (MQ=255)
                                                                                                            cgccgGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTAc                                                                                                         <  1:348916/53‑1 (MQ=255)
                                                                                                            cgccgGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTAc                                                                                                         >  2:348916/1‑53 (MQ=255)
                                                                                                            cgccgGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCa                                                                                            >  2:42692/1‑66 (MQ=255)
                                                                                                            cgccaGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCa                                                                                            <  1:42692/66‑1 (MQ=255)
                                                                                                               cgGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagcc                                                                           <  2:269798/80‑1 (MQ=255)
                                                                                                               cgGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagcc                                                                           >  1:269798/1‑80 (MQ=255)
                                                                                                                       gTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAg                                                                                                            <  1:816309/39‑1 (MQ=255)
                                                                                                                       gTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAg                                                                                                            >  2:816309/1‑39 (MQ=255)
                                                                                                                           tGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacag                                                                             >  2:119923/1‑66 (MQ=255)
                                                                                                                           tGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacag                                                                             <  1:119923/66‑1 (MQ=255)
                                                                                                                           tGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcat                                                                       <  1:701612/72‑1 (MQ=255)
                                                                                                                           tGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcat                                                                       >  2:701612/1‑72 (MQ=255)
                                                                                                                             aCATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTcc  <  2:51490/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
GCTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCC  >  NC_000913/1763853‑1764117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: