Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,214,339 (C)6→5 coding (306/1686 nt) yehU ← inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,214,3340C.98.3% 234.4 / ‑2.7 58coding (311/1686 nt)yehUinner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (30/27);  minor base A (0/1);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACC  >  NC_000913/2214198‑2214463
                                                                                                                                        |                                                                                                                                 
gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 <  2:590045/139‑1 (MQ=255)
   gaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccccca                                                                                                                              <  2:1437654/139‑2 (MQ=255)
    aaCGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccat                                                                                                                             >  2:1164644/1‑137 (MQ=255)
                             ggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccatc                                                                                                                            <  2:1576358/115‑4 (MQ=255)
                             ggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccatc                                                                                                                            >  1:1576358/1‑112 (MQ=255)
                             ggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAata                                                                                                                      <  2:1632353/120‑1 (MQ=255)
                             ggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAata                                                                                                                      >  1:1632353/1‑120 (MQ=255)
                                tAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                <  1:885282/139‑1 (MQ=255)
                                     aCTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                           <  1:1164644/139‑1 (MQ=255)
                                         tATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATACCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                       <  1:1180036/139‑1 (MQ=255)
                                                 cgcCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 >  2:667684/1‑90 (MQ=255)
                                                 cgcCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 <  1:667684/90‑1 (MQ=255)
                                                   cccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgacga                                                                             >  1:1586327/1‑139 (MQ=255)
                                                        ggcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccgga                                                                        <  1:547551/139‑1 (MQ=255)
                                                         gcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTa                                                                                                              <  1:1466991/100‑1 (MQ=255)
                                                         gcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTa                                                                                                              >  2:1466991/1‑100 (MQ=255)
                                                         gcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggac                                                                       >  1:909132/1‑139 (MQ=255)
                                                                         tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 >  2:73246/1‑66 (MQ=255)
                                                                         tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 <  1:73246/66‑1 (MQ=255)
                                                                         tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                           <  1:346680/103‑1 (MQ=255)
                                                                         tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                           >  2:346680/1‑103 (MQ=255)
                                                                         tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                       >  1:92833/1‑139 (MQ=255)
                                                                            aCCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                            >  2:80059/1‑131 (MQ=255)
                                                                            aCCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                            <  1:80059/131‑1 (MQ=255)
                                                                                 gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                            >  1:1609342/1‑126 (MQ=255)
                                                                                 gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                            <  2:1609342/126‑1 (MQ=255)
                                                                                 gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                               <  1:1412102/139‑1 (MQ=255)
                                                                                 gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                               <  2:665791/139‑1 (MQ=255)
                                                                                   cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                             >  2:1376745/1‑139 (MQ=255)
                                                                                     ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                           <  1:1529783/91‑1 (MQ=255)
                                                                                     ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                           >  2:1529783/1‑91 (MQ=255)
                                                                                     ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgc                                              <  1:884834/136‑1 (MQ=255)
                                                                                     ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgc                                              >  2:884834/1‑136 (MQ=255)
                                                                                     ccGAGTAATCCTTCAACGATGGTCGAGATCATGCACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCt                                          >  2:954609/1‑139 (MQ=255)
                                                                                            aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    <  2:1586327/139‑1 (MQ=255)
                                                                                            aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    <  2:92833/139‑1 (MQ=255)
                                                                                             tCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCAc                                   <  1:1376745/139‑1 (MQ=255)
                                                                                                   aaCGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                       <  2:457569/81‑1 (MQ=255)
                                                                                                   aaCGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                       >  1:457569/1‑81 (MQ=255)
                                                                                                        tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                            <  2:402547/103‑1 (MQ=255)
                                                                                                        tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                            >  1:402547/1‑103 (MQ=255)
                                                                                                        tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                        >  1:236575/1‑139 (MQ=255)
                                                                                                        tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                        <  2:771484/139‑1 (MQ=255)
                                                                                                          gTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGTCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAAt                      >  2:558712/1‑139 (MQ=255)
                                                                                                          gTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCTGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTAtt                            >  2:1105103/1‑133 (MQ=255)
                                                                                                          gTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTAtt                            <  1:1105103/133‑1 (MQ=255)
                                                                                                          gTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAAt                      >  1:211228/1‑139 (MQ=255)
                                                                                                           tCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATa                     >  1:128815/1‑139 (MQ=255)
                                                                                                            cGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAg                    >  1:399521/1‑139 (MQ=255)
                                                                                                                    tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                     >  1:1138742/1‑50 (MQ=255)
                                                                                                                    tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                     <  2:1138742/50‑1 (MQ=255)
                                                                                                                    tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                               <  2:1665426/104‑1 (MQ=255)
                                                                                                                    tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                               >  1:1665426/1‑104 (MQ=255)
                                                                                                                    tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    >  1:1498455/1‑115 (MQ=255)
                                                                                                                    tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    <  2:1498455/115‑1 (MQ=255)
                                                                                                                    tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa            >  2:1662788/1‑139 (MQ=255)
                                                                                                                     gCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCaa           >  1:1485686/1‑139 (MQ=255)
                                                                                                                         cTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                     <  1:75231/45‑1 (MQ=255)
                                                                                                                         cTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                     >  2:75231/1‑45 (MQ=255)
                                                                                                                         cTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTAtt                            <  1:291394/118‑1 (MQ=255)
                                                                                                                         cTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTAtt                            >  2:291394/1‑118 (MQ=255)
                                                                                                                         cTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg       <  2:399521/139‑1 (MQ=255)
                                                                                                                         cTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg       <  2:128815/139‑1 (MQ=255)
                                                                                                                           tAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCAtt                                                   <  2:1211162/93‑1 (MQ=255)
                                                                                                                           tAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCAtt                                                   >  1:1211162/1‑93 (MQ=255)
                                                                                                                             aCGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAAc   >  2:1485458/1‑139 (MQ=255)
                                                                                                                              cgcgGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAAcc  >  2:1419059/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                 
GACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACC  >  NC_000913/2214198‑2214463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: