Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,860,426 Δ1 bp intergenic (+10/+41) pphB → / ← ygbI serine/threonine‑specific protein phosphatase 2/DeoR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,860,4250T.100.0% 174.2 / NA 42intergenic (+9/+42)pphB/ygbIserine/threonine‑specific protein phosphatase 2/DeoR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (20/22);  total (20/22)

TATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATA  >  NC_000913/2860308‑2860551
                                                                                                                     |                                                                                                                              
tataTTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAc                                                                                                          <  2:264332/139‑1 (MQ=255)
 atatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                                         <  1:662836/139‑1 (MQ=255)
   atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGct                                                                                                                                >  2:1609614/1‑115 (MQ=255)
   atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGct                                                                                                                                <  1:1609614/115‑1 (MQ=255)
   atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCg                                                                                                       >  1:874914/1‑139 (MQ=255)
       ggACATATGATGTTTGATAACATTCAGAGGTTCGATAACCAGATTTATAATGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCAgtgt                                                                                                   >  1:1081330/1‑139 (MQ=255)
            tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCCTGCTCTTCTGTGAAGCCTGa                                                                                                               <  2:1135516/122‑1 (MQ=255)
            tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGa                                                                                                               >  1:1135516/1‑122 (MQ=255)
              tgatgTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCa                                                                                            >  1:681684/1‑139 (MQ=255)
                       atatCATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGcc                                                                                        >  1:1076965/1‑134 (MQ=255)
                       aTAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGt                                                                                                             <  2:1468664/113‑1 (MQ=255)
                       aTAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGt                                                                                                             >  1:1468664/1‑113 (MQ=255)
                       aTAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGcc                                                                                        <  2:1076965/134‑1 (MQ=255)
                       aTAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCattat                                                                                   >  2:1414939/1‑139 (MQ=255)
                                    gTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                      <  1:727707/139‑1 (MQ=255)
                                      tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGct                                                                                                                                <  1:242486/80‑1 (MQ=255)
                                      tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGct                                                                                                                                >  2:242486/1‑80 (MQ=255)
                                      tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCAt                                                                                                                 <  1:514806/94‑1 (MQ=255)
                                      tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCAt                                                                                                                 >  2:514806/1‑94 (MQ=255)
                                      tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                                   <  1:1094465/108‑1 (MQ=255)
                                      tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                                   >  2:1094465/1‑108 (MQ=255)
                                      tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGc                                                                        >  1:1635620/1‑135 (MQ=255)
                                      tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGc                                                                        <  2:1635620/135‑1 (MQ=255)
                                      tCGATAACCAGCTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCAGTGTTATCGCAGGCCATTATTCATTTTTCGCTAcc                                                                    <  2:1081330/139‑1 (MQ=255)
                                                gATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                        >  2:923624/1‑93 (MQ=255)
                                                gATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                        <  1:923624/93‑1 (MQ=255)
                                                gATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAagaga                                                          <  2:681684/139‑1 (MQ=255)
                                                 aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtg                                                                                                                         >  2:649418/1‑75 (MQ=255)
                                                 aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtg                                                                                                                         <  1:649418/75‑1 (MQ=255)
                                                   ttATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                                       <  2:1584246/139‑1 (MQ=255)
                                                           aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGt                                                                                                             <  2:1589933/77‑1 (MQ=255)
                                                           aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGt                                                                                                             >  1:1589933/1‑77 (MQ=255)
                                                           aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                        <  2:722514/82‑1 (MQ=255)
                                                           aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                        >  1:722514/1‑82 (MQ=255)
                                                           aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAcc                                                                    <  2:161205/118‑1 (MQ=255)
                                                           aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAcc                                                                    >  1:161205/1‑118 (MQ=255)
                                                            tACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCa                                                                                       <  1:618330/98‑1 (MQ=255)
                                                            tACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCa                                                                                       >  2:618330/1‑98 (MQ=255)
                                                                              cgggcggCTGTCATTTTATAAAATAAAGTATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                                       <  2:1280170/111‑1 (MQ=255)
                                                                              cgggcggCTGTCATTTTATAAAATAAAGTATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                                       >  1:1280170/1‑111 (MQ=255)
                                                                                ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                                         >  1:1087038/1‑60 (MQ=255)
                                                                                ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                                         <  2:1087038/60‑1 (MQ=255)
                                                                                                taaaataaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt          <  2:350501/139‑1 (MQ=255)
                                                                                                        agtaatCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCAGGCAAACCGTCGTCTGtaata  >  1:1438823/1‑139 (MQ=255)
                                                                                                              ctcATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                                       >  1:1137004/1‑80 (MQ=255)
                                                                                                              ctcATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                                       <  2:1137004/80‑1 (MQ=255)
                                                                                                                     |                                                                                                                              
TATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATA  >  NC_000913/2860308‑2860551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: