Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,212,088 (C)8→9 intergenic (‑85/+615) iraM ← / ← ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,212,0801.C94.1% 47.3 / ‑3.1 17intergenic (‑77/+623)iraM/ycgXRpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (11/5);  total (12/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑‑CCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTT  >  NC_000913/1211984‑1212206
                                                                                                  |                                                                                                                               
tCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATAAAAATATTGGCTta                                                                                       >  2:147856/1‑139 (MQ=255)
                    ttGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGc                                                                   >  1:1276850/1‑139 (MQ=255)
                     tGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCa                                                                  <  2:1276850/139‑1 (MQ=255)
                         aTGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                               >  1:212763/1‑106 (MQ=255)
                         aTGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                               <  2:212763/106‑1 (MQ=255)
                                      cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCCCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATg                                                 >  1:687741/1‑139 (MQ=255)
                                           tttAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGAAATCTCAATGGATGTTaaa                                            >  2:343155/1‑139 (MQ=255)
                                                     ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATgaga                                  >  2:333871/1‑139 (MQ=255)
                                                     ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATgaga                                  >  2:870322/1‑139 (MQ=255)
                                                              aTGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                               >  1:1664332/1‑69 (MQ=255)
                                                              aTGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                               <  2:1664332/69‑1 (MQ=255)
                                                               cgTTTATTTCTTGATTTTGCATATGAGTATATTAA‑‑ACCCCCCCTCCAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAACTTAAAAGCAAGAAATCTCAATGGATGTTAAACAATATGAGATTTTGTGaaa                        >  1:63666/2‑139 (MQ=255)
                                                                           gATTTTGCATATGAGTATATTACCCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAatta             >  1:1270112/1‑139 (MQ=255)
                                                                           gATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                               <  1:903511/56‑1 (MQ=255)
                                                                           gATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                               >  2:903511/1‑56 (MQ=255)
                                                                                     atGAATATTTTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGtt  <  1:147856/139‑1 (MQ=255)
                                                                                          tataTTACCCCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                               >  1:263825/1‑41 (MQ=38)
                                                                                          tataTTACCCCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                               <  2:263825/41‑1 (MQ=38)
                                                                                                  |                                                                                                                               
TCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑‑CCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTT  >  NC_000913/1211984‑1212206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: