Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,399,985 (C)7→8 coding (252/516 nt) ogt ← O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,399,9781.C100.0% 121.5 / NA 34T87A (ACG→GCG) ogtO‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (16/18);  total (16/18)

GGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC  >  NC_000913/1399841‑1400111
                                                                                                                                          |                                                                                                                                      
gggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑c                                                                                                                                      <  2:971621/139‑1 (MQ=255)
 ggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑cc                                                                                                                                     <  2:191482/139‑1 (MQ=255)
 ggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑cc                                                                                                                                     <  1:761521/139‑1 (MQ=255)
   aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑cccc                                                                                                                                   <  2:782387/139‑1 (MQ=255)
        aTCCGTTTGCCGCACCAACGGCACGCGCTGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccccccg                                                                                                                              <  2:1002736/139‑3 (MQ=255)
        aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccccccg                                                                                                                              <  1:102590/139‑3 (MQ=255)
           cGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccccccgtag                                                                                                                           <  1:408637/139‑6 (MQ=255)
            gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGc                                                                                                                           <  2:157842/139‑1 (MQ=255)
            gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGc                                                                                                                           <  1:1343023/139‑1 (MQ=255)
                   gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGa                                                                                                                    <  2:739593/139‑1 (MQ=255)
                   gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGa                                                                                                                    <  1:937839/139‑1 (MQ=255)
                   gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGa                                                                                                                    <  1:1149257/139‑1 (MQ=255)
                     aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAg                                                                                                                  <  2:27581/139‑1 (MQ=255)
                        aaCGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGt                                                                                                               <  1:1306833/139‑1 (MQ=255)
                                                 cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGc                                                                                                                           <  2:894557/102‑1 (MQ=255)
                                                 cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGc                                                                                                                           >  1:894557/1‑102 (MQ=255)
                                                            aGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccccccg                                                                                                                              >  2:1154780/1‑85 (MQ=255)
                                                            aGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccccccg                                                                                                                              <  1:1154780/87‑3 (MQ=255)
                                                              ccAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTaa                                                                                                 >  2:895573/1‑115 (MQ=255)
                                                              ccAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTaa                                                                                                 <  1:895573/115‑1 (MQ=255)
                                                                                ttACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCg                                                        >  1:366624/1‑139 (MQ=255)
                                                                                ttACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTggg                                                                                                                     <  2:686147/77‑1 (MQ=255)
                                                                                ttACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTggg                                                                                                                     >  1:686147/1‑77 (MQ=255)
                                                                                 tACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGcc                                                      <  2:1655859/139‑1 (MQ=255)
                                                                                 tACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑cccccccccgtag                                                                                                                          >  1:1534774/1‑64 (MQ=255)
                                                                                 tACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑cccccccccgtag                                                                                                                          <  2:1534774/70‑7 (MQ=255)
                                                                                       cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAtt                                                >  1:139691/1‑139 (MQ=255)
                                                                                       cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccccccgta                                                                                                                            <  2:467718/62‑5 (MQ=255)
                                                                                       cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccccccgta                                                                                                                            >  1:467718/1‑58 (MQ=255)
                                                                                           cAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAAcc                                                         <  2:984042/126‑1 (MQ=255)
                                                                                           cAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAAcc                                                         >  1:984042/1‑126 (MQ=255)
                                                                                                     tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGt                                                                                                                                         >  2:506778/1‑37 (MQ=255)
                                                                                                     tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGt                                                                                                                                         <  1:506778/37‑1 (MQ=255)
                                                                                                        gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCt                               >  1:855355/1‑139 (MQ=255)
                                                                                                        gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGC‑‑AAACCGCCTGGATTGGTGGCAGAAATGCGCTCa                             >  2:494671/1‑139 (MQ=255)
                                                                                                                 tCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCctt                      >  2:1283261/1‑139 (MQ=255)
                                                                                                                   cAGACTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCcttct                    <  2:855355/139‑1 (MQ=255)
                                                                                                                      aCTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt                                                                     >  1:1125106/1‑87 (MQ=255)
                                                                                                                      aCTTCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt                                                                     <  2:1125106/87‑1 (MQ=255)
                                                                                                                        ttCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaaa                                                           >  2:1393662/1‑95 (MQ=255)
                                                                                                                        ttCGCGCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaaa                                                           <  1:1393662/95‑1 (MQ=255)
                                                                                                                          cgcgCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt                                                                     <  2:1028373/83‑1 (MQ=255)
                                                                                                                          cgcgCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt                                                                     >  1:1028373/1‑83 (MQ=255)
                                                                                                                          cgcgCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt             >  1:8369/1‑139 (MQ=255)
                                                                                                                          cgcgCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt             >  2:754175/1‑139 (MQ=255)
                                                                                                                          cgcgCTGAAATGGCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt             >  2:1025429/1‑139 (MQ=255)
                                                                                                                                     ggCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGc                                                                                       >  1:1329842/1‑54 (MQ=255)
                                                                                                                                     ggCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGc                                                                                       <  2:1329842/54‑1 (MQ=255)
                                                                                                                                     ggCGTC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTcc  <  1:1283261/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
GGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC  >  NC_000913/1399841‑1400111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑