Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,600,609	(C)5→6	coding (132/471 nt)	bcp →	peroxiredoxin, thiol peroxidase, thioredoxin‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,600,604	1	.	C	100.0%	189.2 / NA	54	T43P (ACC→CCC)	bcp	peroxiredoxin, thiol peroxidase, thioredoxin‑dependent
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (28/26);  total (28/26)

TGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑CCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAGAG  >  NC_000913/2600479‑2600732                                                                                                                               |                                                                                                                                 tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      <  2:1054916/139‑1 (MQ=255) tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      >  1:383097/1‑139 (MQ=255) tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      <  1:219038/139‑1 (MQ=255)     tCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGt                                                                                                                  >  2:758989/1‑139 (MQ=255)        aCTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACa                                                                                                               <  2:580055/139‑1 (MQ=255)        aCTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                          >  2:783797/1‑124 (MQ=255)        aCTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                          <  1:783797/127‑4 (MQ=255)             aaGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCt                                                                                                          <  2:383097/139‑1 (MQ=255)                     gATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTa                                                                                                  <  1:758989/139‑1 (MQ=255)                      atatCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTAc                                                                                                 <  1:868851/139‑1 (MQ=255)                        atCGCACGGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTAcgc                                                                                               <  1:1245731/139‑1 (MQ=255)                                 aaaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                          <  1:1109270/102‑4 (MQ=255)                                 aaaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                          >  2:1109270/1‑99 (MQ=255)                                                           agaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa                                                                                             <  1:535341/106‑1 (MQ=255)                                                           agaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa                                                                                             >  2:535341/1‑106 (MQ=255)                                                           agaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt                                                                                   >  2:1138702/1‑116 (MQ=255)                                                           agaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt                                                                                   <  1:1138702/116‑1 (MQ=255)                                                           agaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                          >  2:159098/1‑73 (MQ=255)                                                           agaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                          <  1:159098/76‑4 (MQ=255)                                                           agaACAAGTTAATTTGACCGACTTCCAAGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCggg                                                                  >  1:1642189/1‑133 (MQ=255)                                                           agaACAAGTTAATTTGACCGACTTCCAAGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCggg                                                                  <  2:1642189/133‑1 (MQ=255)                                                             aaCAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa                                                                                             >  2:697257/1‑104 (MQ=255)                                                             aaCAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa                                                                                             <  1:697257/104‑1 (MQ=255)                                                             aaCAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtg                                                          >  2:1509443/1‑139 (MQ=255)                                                             aaCAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑cccc                                                                                                                              <  2:526666/70‑1 (MQ=255)                                                             aaCAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑cccc                                                                                                                              >  1:526666/1‑70 (MQ=255)                                                                aaGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGc                                                       >  2:1302076/1‑139 (MQ=255)                                                                  gTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTg                                                     >  2:1456575/1‑139 (MQ=255)                                                                       tttGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTAt                                                <  2:535553/139‑1 (MQ=255)                                                                           aCCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTg                                                                                                         <  2:233684/78‑1 (MQ=255)                                                                           aCCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTg                                                                                                         >  1:233684/1‑78 (MQ=255)                                                                           aCCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGc                                                                     <  2:1392398/114‑1 (MQ=255)                                                                           aCCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGc                                                                     >  1:1392398/1‑114 (MQ=255)                                                                             cGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCAc                                          >  1:1606774/1‑139 (MQ=255)                                                                                        gACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCg                               <  2:1606774/139‑1 (MQ=255)                                                                                           aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGc                                                       >  1:734134/1‑112 (MQ=255)                                                                                           aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGc                                                       <  2:734134/112‑1 (MQ=255)                                                                                             cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTa                                                                                                                 <  1:472763/52‑1 (MQ=255)                                                                                             cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTa                                                                                                                 >  2:472763/1‑52 (MQ=255)                                                                                             cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt                                                                                   >  2:1496132/1‑82 (MQ=255)                                                                                             cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt                                                                                   <  1:1496132/82‑1 (MQ=255)                                                                                             cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGc                                                                     >  2:750976/1‑96 (MQ=255)                                                                                             cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGc                                                                     <  1:750976/96‑1 (MQ=255)                                                                                             cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtgtg                                                        >  2:1440739/1‑109 (MQ=255)                                                                                             cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtgtg                                                        <  1:1440739/109‑1 (MQ=255)                                                                                             cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTAt                                                <  1:1556747/117‑1 (MQ=255)                                                                                             cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTAt                                                >  2:1556747/1‑117 (MQ=255)                                                                                             cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATaa                                    <  2:563420/129‑1 (MQ=255)                                                                                             cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATaa                                    >  1:563420/1‑129 (MQ=255)                                                                                             cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaaaa                          >  2:929908/1‑139 (MQ=255)                                                                                              gtgtTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCtt                                                                                                   >  1:87878/1‑65 (MQ=255)                                                                                              gtgtTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCtt                                                                                                   <  2:87878/65‑1 (MQ=255)                                                                                                gtTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAActc                       >  1:997523/1‑139 (MQ=255)                                                                                                  tCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCggg                                                                  >  1:933648/1‑94 (MQ=255)                                                                                                  tCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCggg                                                                  <  2:933648/94‑1 (MQ=255)                                                                                                  tCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACtctc                     >  2:1078555/1‑139 (MQ=255)                                                                                                   cTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTcc                    >  2:672960/1‑139 (MQ=255)                                                                                                        ttATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGttt               <  1:1509443/139‑1 (MQ=255)                                                                                                         tATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGtttt              >  2:275891/1‑139 (MQ=255)                                                                                                           tttCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGc            >  2:50940/1‑139 (MQ=255)                                                                                                                     aaaGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAagag  >  1:644584/1‑139 (MQ=255)                                                                                                                     aaaGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAagag  <  1:1078555/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                 TGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑CCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAGAG  >  NC_000913/2600479‑2600732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑