Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,330,357 +T coding (1395/1434 nt) dacB → D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,330,3531.T100.0% 196.7 / NA 62R464L (CGT→CTT) dacBD‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (29/33);  total (29/33)

TGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCC  >  NC_000913/3330224‑3330486
                                                                                                                                  |                                                                                                                                      
gcaggggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑‑tttttgaaa                                                                                                                              <  2:651017/135‑6 (MQ=255)
     ggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCGACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          <  1:1087497/139‑1 (MQ=255)
     ggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          <  2:1319737/139‑1 (MQ=255)
     ggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          >  1:438115/1‑139 (MQ=255)
      gggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGtt                                                                                                                         <  2:438115/139‑1 (MQ=255)
       ggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑‑tttttga                                                                                                                                <  2:1326470/130‑4 (MQ=255)
       ggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑‑tttttga                                                                                                                                >  1:1326470/1‑127 (MQ=255)
             taACCTGGCGGGATTCATTACCACAGCGAGAGGGCAACGAATGTCGTTTGTGCAATATCTTTCTGGGTATGCAGTAGAACCTGGGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGACAGCCATTTCTATaa                                                                                                                  <  1:1180323/139‑1 (MQ=255)
               aCCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCg                                                                                                                                         <  2:949793/115‑1 (MQ=255)
               aCCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCg                                                                                                                                         >  1:949793/1‑115 (MQ=255)
                   ggCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATaa                                                                                                                  <  1:946782/133‑1 (MQ=255)
                   ggCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGATATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATaa                                                                                                                  >  2:946782/1‑133 (MQ=255)
                     cGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGc                                                                                                                             <  2:783201/120‑1 (MQ=255)
                     cGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGc                                                                                                                             >  1:783201/1‑120 (MQ=255)
                         aTTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATaa                                                                                                                  >  2:790300/1‑127 (MQ=255)
                         aTTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATaa                                                                                                                  <  1:790300/127‑1 (MQ=255)
                               tACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑‑tttttgaaa                                                                                                                              >  2:831527/1‑103 (MQ=255)
                               tACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑‑tttttgaaa                                                                                                                              <  1:831527/108‑6 (MQ=255)
                                         agcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAg                                                                                                     >  2:839520/1‑124 (MQ=255)
                                         agcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCaaaa                                                                                      >  2:1434995/1‑139 (MQ=255)
                                         agcgGGAAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAg                                                                                                     <  1:839520/124‑1 (MQ=255)
                                            gggCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa                                                                                   <  1:1434995/139‑1 (MQ=255)
                                                       ggCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          <  2:42667/89‑1 (MQ=255)
                                                       ggCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          >  1:42667/1‑89 (MQ=255)
                                                             tgtgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATaa                                                                                                                  >  2:1197765/1‑91 (MQ=255)
                                                             tgtgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATaa                                                                                                                  <  1:1197765/91‑1 (MQ=255)
                                                                 cAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAg                                                                                                     <  1:1443871/100‑1 (MQ=255)
                                                                 cAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAg                                                                                                     >  2:1443871/1‑100 (MQ=255)
                                                                 cAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCgggg                                                              <  1:720842/139‑1 (MQ=255)
                                                                      tCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATTGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          <  2:1339751/74‑1 (MQ=255)
                                                                      tCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATTGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          >  1:1339751/1‑74 (MQ=255)
                                                                          tCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑‑tttttgaaa                                                                                                                              >  2:127011/1‑60 (MQ=255)
                                                                          tCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑‑tttttgaaa                                                                                                                              <  1:127011/65‑6 (MQ=255)
                                                                                  tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATAtt                                                                                                           >  1:39709/1‑77 (MQ=255)
                                                                                  tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATAtt                                                                                                           >  1:58829/1‑77 (MQ=255)
                                                                                  tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATAtt                                                                                                           <  2:39709/77‑1 (MQ=255)
                                                                                  tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATAtt                                                                                                           <  2:58829/77‑1 (MQ=255)
                                                                                   gCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAccc                                                                              >  1:656326/1‑105 (MQ=255)
                                                                                   gCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAccc                                                                              <  2:656326/105‑1 (MQ=255)
                                                                                          aaCCTGCGGGTCAAGTTAATTGCCGTATTCCTTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt                                     <  1:990059/139‑1 (MQ=255)
                                                                                            ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          >  1:388396/1‑52 (MQ=39)
                                                                                            ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          <  2:388396/52‑1 (MQ=39)
                                                                                            ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATaa                                                                                                                  <  2:1510933/60‑1 (MQ=255)
                                                                                            ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATaa                                                                                                                  >  1:1510933/1‑60 (MQ=255)
                                                                                                 ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          >  2:1658100/1‑47 (MQ=38)
                                                                                                 ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          <  2:1179126/47‑1 (MQ=38)
                                                                                                 ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          <  1:1658100/47‑1 (MQ=38)
                                                                                                 ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCAGt                                                                                                                          >  1:1179126/1‑47 (MQ=37)
                                                                                                   aTCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGca                                                                         >  1:1269678/1‑94 (MQ=255)
                                                                                                   aTCAGCGTAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGca                                                                         <  2:1269678/94‑1 (MQ=255)
                                                                                                          tAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATaa                                                                                                                  <  2:719189/46‑1 (MQ=39)
                                                                                                          tAATCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATaa                                                                                                                  >  1:719189/1‑46 (MQ=255)
                                                                                                            aTCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATg                                                                     <  1:20965/89‑1 (MQ=255)
                                                                                                            aTCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATg                                                                     >  2:20965/1‑89 (MQ=255)
                                                                                                             tCGCCGTATTCCGTTAGTGCGTTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                   <  2:31379/139‑1 (MQ=39)
                                                                                                             tCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          <  2:1172429/35‑1 (MQ=37)
                                                                                                             tCGCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGt                                                                                                                          >  1:1172429/1‑35 (MQ=37)
                                                                                                               gCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGtat                                                                                                                    >  2:1636610/1‑39 (MQ=37)
                                                                                                               gCCGTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGtat                                                                                                                    <  1:1636610/39‑1 (MQ=37)
                                                                                                                  gTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                   <  1:508859/133‑1 (MQ=255)
                                                                                                                  gTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                   >  2:508859/1‑133 (MQ=255)
                                                                                                                  gTATTCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa             >  1:472039/1‑139 (MQ=255)
                                                                                                                      tCCGTTAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGc         <  2:1085735/139‑1 (MQ=255)
                                                                                                                          ttAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACGTGGCCGGGGCTTCAGat                                                      >  2:1016653/1‑90 (MQ=255)
                                                                                                                          ttAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGcc                                                                  <  1:432262/78‑1 (MQ=255)
                                                                                                                          ttAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGcc                                                                  >  2:432262/1‑78 (MQ=255)
                                                                                                                          ttAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGat                                                      <  1:1016653/90‑1 (MQ=255)
                                                                                                                          ttAGTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTa     >  2:455079/1‑139 (MQ=255)
                                                                                                                             gTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGcc                                                                  <  1:1273803/75‑1 (MQ=255)
                                                                                                                             gTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGcc                                                                  >  2:1273803/1‑75 (MQ=255)
                                                                                                                             gTGCGT‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATcc  >  1:1599197/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                      
TGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCC  >  NC_000913/3330224‑3330486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑