| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,511,746 | (A)8→9 | coding (599/906 nt) | yhfZ ← | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,511,738 | 1 | . | A | 98.2% | 181.1 / ‑3.7 | 57 | coding (607/906 nt) | yhfZ | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (29/27); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTC > NC_000913/3511604‑3511867 | cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaa > 2:850245/1‑139 (MQ=255) aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATg > 2:1513690/1‑139 (MQ=255) aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATg < 1:1513690/139‑1 (MQ=255) cATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAACACATCGGTCATGA‑t > 1:431363/1‑139 (MQ=255) tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCt > 2:12873/1‑139 (MQ=255) tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATccgccg < 2:431363/139‑1 (MQ=255) tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATccgccg < 1:850245/139‑1 (MQ=255) gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACgg < 2:78289/139‑1 (MQ=255) aTCACCGCATCGACATCGCTTTTAACAATGCGTTGTAAACTCTTGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaa < 1:584769/90‑1 (MQ=255) aTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaa > 2:584769/1‑90 (MQ=255) aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACgcgc > 2:989534/1‑139 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt > 1:1069516/1‑139 (MQ=255) cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCCCTCCCCCCA‑AAAAAAA‑ACACCGG‑CCTGATTTTTTTGACCCCCCGACCGGTTTTCAACCCCCACCCCCTTCa > 2:664277/1‑139 (MQ=255) tttAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACgg < 2:615191/104‑1 (MQ=255) tttAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACgg > 1:615191/1‑104 (MQ=255) tttAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc < 2:1069516/139‑1 (MQ=255) gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTc > 2:454771/1‑71 (MQ=255) gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTc < 1:454771/71‑1 (MQ=255) tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 1:1158698/1‑94 (MQ=255) tGATAAGAGAGATCGACTCGTTCCACAGCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 2:1158698/94‑1 (MQ=255) aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 1:12873/139‑1 (MQ=255) agagagATCGACTCGTTCCACATCACTACCGCCAAAAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 2:1355123/1‑90 (MQ=255) agagagATCGACTCGTTCCACATCACTACCGCCAAAAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 1:1355123/90‑1 (MQ=255) agagagATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGcc > 2:31024/1‑139 (MQ=255) gaTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGt > 1:1226747/1‑139 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGt > 2:1295924/1‑139 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATccg > 1:608725/1‑60 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATccg < 2:608725/60‑1 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACgg < 2:335455/68‑1 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACgg > 1:335455/1‑68 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 1:716828/1‑83 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 2:716828/83‑1 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt > 1:1480412/1‑102 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt < 2:1480412/102‑1 (MQ=255) tCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc < 1:1547043/113‑1 (MQ=255) tCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc > 2:1547043/1‑113 (MQ=255) gACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCaa < 2:1507826/113‑1 (MQ=255) gACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCaa > 1:1507826/1‑113 (MQ=255) aCTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGAt < 1:804553/53‑1 (MQ=255) aCTCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGAt > 2:804553/1‑53 (MQ=255) tCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc > 1:727974/1‑116 (MQ=255) tCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc < 2:727974/116‑1 (MQ=255) tCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCa > 2:440628/1‑128 (MQ=255) tCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCa < 1:440628/128‑1 (MQ=255) tCGTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTCTACGGCAAATGAACTGGTGCTCGCCAACGTAGGtgtg > 1:1063448/1‑139 (MQ=255) gTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc < 1:1173282/95‑1 (MQ=255) gTTCCACATCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc > 2:1173282/1‑95 (MQ=255) aTCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 2:390411/68‑1 (MQ=255) aTCACTACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 1:390411/1‑68 (MQ=255) cTACCGCCAAAAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTc < 1:1295924/139‑1 (MQ=255) tACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACgg > 2:499625/1‑48 (MQ=255) tACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACgg < 1:499625/48‑1 (MQ=255) tACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTTCGGACTCGCCTTTACGGCAAATCAACTGGTg > 1:1397884/1‑107 (MQ=255) tACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc < 2:231433/94‑1 (MQ=255) tACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc > 1:231433/1‑94 (MQ=255) tACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg < 2:1397884/107‑1 (MQ=255) tACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg < 2:1113607/125‑1 (MQ=255) tACCGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg > 1:1113607/1‑125 (MQ=255) ccGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc < 2:348006/89‑1 (MQ=255) ccGCCA‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc > 1:348006/1‑89 (MQ=255) | CGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAA‑ACATCGGTCATGA‑TTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTC > NC_000913/3511604‑3511867 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |