Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,980,957	(G)6→7	coding (71/1386 nt)	yifK →	putative APC family amino acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,980,951	1	.	G	100.0%	232.8 / NA	65	L22R (CTG→CGG)	yifK	putative APC family amino acid transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (31/34);  total (31/34)

ACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCA  >  NC_000913/3980832‑3981072                                                                                                                         |                                                                                                                          aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCAt                                                                                                               <  2:839485/133‑1 (MQ=255) aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCAt                                                                                                               >  1:839485/1‑133 (MQ=255)  cccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTc                                                                                                        <  1:206874/139‑1 (MQ=255)    cATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCgg                                                                                                      >  2:856647/1‑139 (MQ=255)     aTAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGc                                                                                                     <  1:856647/139‑1 (MQ=255)        aCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTg                                                                                                  >  2:445918/1‑139 (MQ=255)           aTAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGttt                                                                                               >  2:1250412/1‑139 (MQ=255)            tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTa                                                                                              >  1:1171916/1‑139 (MQ=255)            tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTa                                                                                              >  1:579418/1‑139 (MQ=255)            tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTa                                                                                              >  1:148209/1‑139 (MQ=255)            tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTa                                                                                              >  2:719165/1‑139 (MQ=255)             aaCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTAt                                                                                             >  2:164366/1‑139 (MQ=255)                   tAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc                                                                                       >  1:1410318/1‑139 (MQ=255)                      cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc                                                                                    <  2:15043/139‑1 (MQ=255)                        ccGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                  <  1:719165/139‑1 (MQ=255)                          ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc                                                                                    <  1:1413870/135‑1 (MQ=255)                          ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc                                                                                    >  2:1413870/1‑135 (MQ=255)                          ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGt                                                                                <  1:164366/139‑1 (MQ=255)                          ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGt                                                                                >  2:207187/1‑139 (MQ=255)                                 aTGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑ggggg                                                                                                                      <  1:1350197/93‑1 (MQ=255)                                 aTGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑ggggg                                                                                                                      >  2:1350197/1‑93 (MQ=255)                                  tGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                  <  2:1293765/129‑1 (MQ=255)                                  tGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                  >  1:1293765/1‑129 (MQ=255)                                   gCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa                                                                       <  2:1171916/139‑1 (MQ=255)                                        cacaacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggc                                                                  <  2:1413299/139‑1 (MQ=255)                                        cacaacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggc                                                                  <  1:207187/139‑1 (MQ=255)                                        cacaacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggc                                                                  >  2:648178/1‑139 (MQ=255)                                          caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCgg                                                                                                      <  1:677288/101‑1 (MQ=255)                                          caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCgg                                                                                                      >  2:677288/1‑101 (MQ=255)                                           aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgg                                                               <  1:1250412/139‑1 (MQ=255)                                           aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgg                                                               <  1:445918/139‑1 (MQ=255)                                             cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggt                                                             <  1:1043119/139‑2 (MQ=255)                                             cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                             <  1:648178/139‑1 (MQ=255)                                                   gATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg                                                       <  1:1336135/139‑1 (MQ=255)                                                       tATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                         >  2:409820/1‑101 (MQ=255)                                                       tATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑ggggg                                                                                                                      >  1:794307/1‑71 (MQ=255)                                                       tATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑ggggg                                                                                                                      <  2:794307/71‑1 (MQ=255)                                                       tATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTAATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                         <  1:409820/101‑1 (MQ=255)                                                         tGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                 <  2:665850/139‑1 (MQ=255)                                                          ggCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgtt                                                <  2:1410318/139‑1 (MQ=255)                                                              gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc                                                                                       <  1:1479417/96‑1 (MQ=255)                                                              gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc                                                                                       >  2:1479417/1‑96 (MQ=255)                                                                      aCCAGAGCTACCGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGTACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa                                                                        <  1:530083/103‑1 (MQ=255)                                                                      aCCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa                                                                        >  2:530083/1‑103 (MQ=255)                                                                          gagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGcc                                                                                                    >  2:911526/1‑71 (MQ=255)                                                                          gagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGcc                                                                                                    <  1:911526/71‑1 (MQ=255)                                                                           agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                         >  1:1198381/1‑81 (MQ=255)                                                                           agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                         <  2:1198381/81‑1 (MQ=255)                                                                           agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTatat                                       >  1:331016/1‑131 (MQ=255)                                                                           agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTatat                                       <  2:331016/131‑1 (MQ=255)                                                                           agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg                               >  1:1254781/1‑139 (MQ=255)                                                                                 aGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGt                         <  2:579418/139‑1 (MQ=255)                                                                                     tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                         <  1:477915/71‑1 (MQ=255)                                                                                     tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                         >  2:477915/1‑71 (MQ=255)                                                                                     tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                             <  1:122899/99‑1 (MQ=255)                                                                                     tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                             >  2:122899/1‑99 (MQ=255)                                                                                     tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGt                     >  2:1324849/1‑139 (MQ=255)                                                                                     tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGt                     >  2:280288/1‑139 (MQ=255)                                                                                       ggCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCt                   <  2:1254781/139‑1 (MQ=255)                                                                                            gAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGt                                                                                                         >  1:8029/1‑48 (MQ=255)                                                                                            gAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGt                                                                                                         <  2:8029/48‑1 (MQ=255)                                                                                             aaGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCggg                             >  2:293022/1‑123 (MQ=255)                                                                                             aaGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCggg                             <  1:293022/123‑1 (MQ=255)                                                                                              aGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcat            <  2:148209/139‑1 (MQ=255)                                                                                                  cGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                  <  2:647381/65‑1 (MQ=255)                                                                                                  cGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                  >  1:647381/1‑65 (MQ=255)                                                                                                        atCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCa  >  2:1157197/1‑139 (MQ=255)                                                                                                                 aTCGCCTTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg                                                                                 <  1:1183173/51‑1 (MQ=255)                                                                                                                 aTCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg                                                                                 >  2:1183173/1‑51 (MQ=255)                                                                                                                         |                                                                                                                          ACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCA  >  NC_000913/3980832‑3981072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑