Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 2,525,917 Δ9 bp intergenic (+26/+5) yfeN → / ← yfeR putative outer membrane protein/transcriptional regulator of yefH

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2525917 2525925 9 81 [0] [0] 67 yfeN/yfeR putative outer membrane protein/transcriptional regulator of yefH

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 25259160 (0.000)65 (0.970) 45/204 0.3 100% intergenic (+25/+14) yfeN/yfeR putative outer membrane protein/transcriptional regulator of yefH
?NC_000913 2525926 = 0 (0.000)intergenic (+35/+4) yfeN/yfeR putative outer membrane protein/transcriptional regulator of yefH

GAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2525780‑2525916
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGG  >  NC_000913/2525926‑2526059
                                                                                                                                                                                                                                                                               
GAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACA                                                                                                                                      >  2:595646/1‑139
     CAACATCACACATTACTACGTGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACAAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTtt                                                                                                                                  <  1:972823/138‑3
     CAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTtta                                                                                                                                 >  1:1517731/1‑136
     CAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTtta                                                                                                                                 >  2:1030805/1‑136
     CAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTtt                                                                                                                                  >  2:972823/1‑136
     CAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTA                                                                                                                                 >  1:359607/1‑139
                        GGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGAGTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCC                                                                                                              >  1:4419/1‑139
                          GGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCC                                                                                                            >  2:477788/1‑139
                            ATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTT                                                                                                          >  2:289128/1‑139
                            ATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATGTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTT                                                                                                          >  1:1454240/1‑139
                                    TCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCA                                                                                                  <  2:1517731/139‑1
                                       ATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATA                                                                                                                               <  2:210254/107‑1
                                       ATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATA                                                                                                                               >  1:210254/1‑107
                                          TGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATT                                                                                            <  1:1190436/139‑1
                                            CGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAG                                                                                          <  1:1138440/139‑1
                                            CGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAG                                                                                          >  1:533268/1‑139
                                            CGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAG                                                                                          >  2:1138440/1‑139
                                                TGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCA                                                                                      >  1:958387/1‑139
                                                TGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCA                                                                                      <  2:4419/139‑1
                                                      ACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCC                                                                                <  1:1030805/139‑1
                                                      ACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCC                                                                                <  1:477788/139‑1
                                                        TGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTG                                                                              <  2:359607/139‑1
                                                         GGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTT                                                                                                          >  1:148987/1‑110
                                                         GGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTT                                                                                                          <  2:148987/110‑1
                                                          GGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGACAC                                                                                                                                       <  2:524496/80‑1
                                                          GGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGACAC                                                                                                                                       >  1:524496/1‑80
                                                            CGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCT                                                                          >  2:1388849/1‑139
                                                                 ATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAC                                                                     <  1:595646/139‑1
                                                                     TTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACG                                                                 <  1:289128/139‑1
                                                                     TTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACG                                                                 <  2:1595769/139‑1
                                                                            GATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAAC                                                                                                                    <  1:1661210/81‑1
                                                                            GATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAAC                                                                                                                    >  2:1661210/1‑81
                                                                                      TCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAG                                                 >  1:882953/1‑138
                                                                                      TCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAG                                                 <  2:882953/138‑1
                                                                                       CTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCG                                               <  2:958387/139‑1
                                                                                           TCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAT                                                                                     <  1:678141/97‑1
                                                                                           TCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAT                                                                                     >  2:678141/1‑97
                                                                                             CATCAAATTTAAGTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCA                                                                                      <  1:988495/94‑1
                                                                                             CATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCA                                                                                      >  2:988495/1‑94
                                                                                               TCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGA                                       >  2:1648529/1‑139
                                                                                               TCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCAT                                                           <  2:250135/119‑1
                                                                                               TCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCAT                                                           >  1:250135/1‑119
                                                                                                CAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGT                                                                <  2:1009444/113‑1
                                                                                                CAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGT                                                                >  1:1009444/1‑113
                                                                                                 AAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCAGTGGAAA                                     <  2:145188/139‑1
                                                                                                     TTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGA                                 <  1:1648529/139‑1
                                                                                                               ataGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATGAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTC                       >  1:970695/4‑139
                                                                                                                  GATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTG                    <  2:970695/139‑1
                                                                                                                  GATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCG                                               <  2:957455/112‑1
                                                                                                                  GATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGAATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCG                                               >  1:957455/1‑112
                                                                                                                   ATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAG                                                 <  1:123492/109‑1
                                                                                                                   ATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAG                                                 >  2:123492/1‑109
                                                                                                                    TTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCG                  >  1:509128/1‑139
                                                                                                                       CTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAC               <  1:1388849/139‑1
                                                                                                                             GGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAG                                                 <  1:737048/99‑1
                                                                                                                             GGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAG                                                 >  2:737048/1‑99
                                                                                                                             GGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCC                                                                                <  1:1545869/68‑1
                                                                                                                             GGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCC                                                                                >  2:1545869/1‑68
                                                                                                                             GGGCTCCCGGCACACTTTATATCTGATACAACGGATCACCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCA                >  1:1119271/1‑132
                                                                                                                             GGGCTCCCCGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCA                <  2:1119271/132‑1
                                                                                                                               GCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCA                                                                                      >  1:1212933/1‑60
                                                                                                                               GCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCA                                                                                      <  2:1212933/60‑1
                                                                                                                                  CCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTG                                                                              >  1:366819/1‑65
                                                                                                                                  CCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTG                                                                              <  2:366819/65‑1
                                                                                                                                    CGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGG  >  2:156672/1‑139
                                                                                                                                                                                                                                                                               
GAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2525780‑2525916
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGG  >  NC_000913/2525926‑2526059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.