| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | NC_000913 | 1,265,001 | 1086 bp→GAGG | [prfA] | [prfA] | |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 1265001 | 1266086 | 1086 | 39 [0] | [0] 38 | [prfA] | [prfA] |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 1265000 | 0 (0.000) | 37 (0.570) +GAGG |
32/196 | 0.8 | 100% | intergenic (+30/‑12) | hemA/prfA | glutamyl tRNA reductase/peptide chain release factor RF‑1 |
| ? | NC_000913 | 1266087 = | 0 (0.000) | coding (1076/1083 nt) | prfA | peptide chain release factor RF‑1 | |||||
AGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGCGTTGTCCGAGCAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTG > NC_000913/1265952‑1266223 | accgcttgatccatgcgccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggagga > 1:1430684‑M2/136‑139 (MQ=255) tgatccatgcgccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGaat < 1:1643205‑M2/6‑1 (MQ=255) tgatccatgcgccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGaat > 2:1643205‑M2/130‑135 (MQ=255) gatccatgcgccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATgg < 2:700812‑M2/11‑1 (MQ=255) atgcgccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAatat > 1:1172013‑M2/124‑139 (MQ=255) atgcgccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAatat < 2:1430684‑M2/16‑1 (MQ=255) ggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGa < 1:1327450‑M2/33‑1 (MQ=255) ggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGa > 2:1327450‑M2/99‑131 (MQ=255) cccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCaa > 2:137787‑M2/94‑139 (MQ=255) cgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACt > 2:259714‑M2/92‑139 (MQ=255) gataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGgcgag > 1:646693‑M2/83‑139 (MQ=255) taacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAaca < 1:1609694‑M2/21‑1 (MQ=255) taacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAaca > 2:1609694‑M2/81‑101 (MQ=255) gcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAg < 1:1231187‑M2/42‑1 (MQ=255) gcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAg > 2:1231187‑M2/73‑114 (MQ=255) cgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGaataat < 2:722155‑M2/9‑1 (MQ=255) cgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGaataat > 1:722155‑M2/59‑67 (MQ=255) cagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGaa < 2:608081‑M2/5‑1 (MQ=255) cagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggAGGaa > 1:608081‑M2/54‑58 (MQ=255) gggtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTg > 1:1001408‑M2/41‑114 (MQ=255) gagtaacagtacatcattttctttttttacagggtggaggAGGaat > 1:14778‑M2/41‑46 (MQ=255) gagtaacagtacatcattttctttttttacagggtggaggAGGaat < 2:14778‑M2/6‑1 (MQ=255) gagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGt > 2:1629159‑M2/41‑139 (MQ=255) gagtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGt < 2:1172013‑M2/99‑1 (MQ=255) gaggaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTg < 2:1001408‑M2/74‑1 (MQ=255) gtaacagtacatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTa < 2:646693‑M2/101‑1 (MQ=255) acatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGtt > 1:697994‑M2/31‑57 (MQ=255) acatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGtt < 2:697994‑M2/27‑1 (MQ=255) acatcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCaga > 2:595778‑M2/31‑139 (MQ=255) catcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCa > 2:1612182‑M2/30‑66 (MQ=255) catcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCa < 1:1612182‑M2/37‑1 (MQ=255) tcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCcg < 1:716125‑M2/67‑1 (MQ=255) tcattttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCcg > 2:716125‑M2/28‑94 (MQ=255) attttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCg < 1:595778‑M2/114‑1 (MQ=255) attttctttttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCg < 1:137787‑M2/114‑1 (MQ=255) ttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCAt < 1:624504‑M2/97‑1 (MQ=255) ttttacagggtggaggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCAt > 2:624504‑M2/17‑113 (MQ=255) ggAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTg < 1:259714‑M2/137‑1 (MQ=255) | AGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGCGTTGTCCGAGCAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTG > NC_000913/1265952‑1266223 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |