BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,518,992	0	C	.	40.2%	227.4 / 156.7	107	pseudogene (272/504 nt)	insB1	IS1 transposase B,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
Reads supporting (aligned to +/- strand): ref base C (34/30); new base . (23/20); total (57/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 111 total aligned reads displayed.

ACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATC  >  NC_000913/4518855‑4519126

aCAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCg                                                                                                                                       >  1:201830/1‑139 (MQ=255)

aCAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCg                                                                                                                                       <  2:131883/139‑1 (MQ=255)

aCAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCg                                                                                                                                       >  2:554409/1‑139 (MQ=255)

                  tGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGta                                                                                                                     <  1:554409/139‑1 (MQ=255)

                  tGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGta                                                                                                                     >  2:1351006/1‑139 (MQ=255)

                            cGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCg                                                                                                                          >  1:590072/1‑124 (MQ=255)

                            cGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCg                                                                                                                          <  2:590072/124‑1 (MQ=255)

                                  cgcTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGTTTGTGGCGCACGTCCTCGGTGAACGCACTCTGGACACACGGGAGCGCCTTCTGAGCCTGCTGTCGGCTTTTGAAGGCGTGGTATAAATGACGGAttgct                                                                                                     >  1:416705/1‑139 (MQ=21)

                                  cgcTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggct                                                                                                     <  1:494471/139‑1 (MQ=255)

                                   gctggctgTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTc                                                                                                                           >  2:833341/1‑116 (MQ=255)

                                     tggctgTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggctggc                                                                                                  <  2:201830/139‑1 (MQ=255)

                                       gctgTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGa                                                                                                                               <  2:25536/108‑1 (MQ=255)

                                       gctgTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGa                                                                                                                               >  1:25536/1‑108 (MQ=255)

                                       gctgTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCg                                                                                                                          >  2:821914/1‑113 (MQ=255)

                                       gctgTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCg                                                                                                                          <  1:821914/113‑1 (MQ=255)

                                                     aTGACAGGCTCCGGAAGACGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGc                                                                                 >  1:778983/1‑139 (MQ=11)

                                                     aTGACAGGCTCCGGAAGACGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGc                                                                                 >  1:50467/1‑139 (MQ=11)

                                                     aTGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCg                                                                                                                          <  2:792531/99‑1 (MQ=255)

                                                     aTGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCg                                                                                                                          >  1:792531/1‑99 (MQ=255)

                                                          aggctccGGAAGACGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGaa                                                                            >  2:824088/7‑139 (MQ=11)

                                                             ctccGGAAGACGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAggg                                                                         >  2:716289/4‑139 (MQ=11)

                                                               ccGGAAGACGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGaa                                                                       >  1:806246/2‑139 (MQ=11)

                                                               aCGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGa                                                                        >  2:1444160/1‑139 (MQ=255)

                                                               aCGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGa                                                                        >  1:321439/1‑139 (MQ=255)

                                                                   aagaCGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCt                                                                   >  1:111257/3‑139 (MQ=14)

                                                                      aCGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCa                                                                <  2:111257/139‑1 (MQ=14)

                                                                      aCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGc                                                                 >  1:175456/1‑139 (MQ=255)

                                                                      aCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGc                                                                 >  2:293138/1‑139 (MQ=255)

                                                                          ttgttgCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGta                                                            >  1:828982/1‑138 (MQ=14)

                                                                          ttgttgCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGta                                                            >  1:161614/1‑138 (MQ=14)

                                                                                 gctcGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGGCGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGc                                                      <  1:1105515/136‑1 (MQ=255)

                                                                                  cACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGAtggctggc                                                                                                  >  1:1506369/1‑93 (MQ=17)

                                                                                  cACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGAtggctggc                                                                                                  <  2:1506369/93‑1 (MQ=17)

                                                                                    cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTc                                                                                                                                        <  1:702194/54‑1 (MQ=255)

                                                                                    cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTc                                                                                                                                        >  2:702194/1‑54 (MQ=255)

                                                                                    cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGc                                                      <  2:1339066/136‑1 (MQ=255)

                                                                                    cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGc                                                      >  1:1339066/1‑136 (MQ=255)

                                                                                    cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAg                                                   >  2:880902/1‑139 (MQ=255)

                                                                                     gtattCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCg                                                 <  2:161614/136‑1 (MQ=14)

                                                                                       cTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCg                                                 >  1:32965/1‑138 (MQ=255)

                                                                                       cTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCg                                                 <  2:32965/138‑1 (MQ=255)

                                                                                        ttCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGtt                                               <  1:880902/139‑1 (MQ=255)

                                                                                        ttCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGta                                                                                                                     >  1:76551/1‑68 (MQ=17)

                                                                                               aaCGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGt                                                                                                                            <  1:317430/55‑1 (MQ=255)

                                                                                               aaCGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGt                                                                                                                            >  2:317430/1‑55 (MQ=255)

                                                                                               aaCGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGta                                                                                                                     <  1:327339/62‑1 (MQ=255)

                                                                                               aaCGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGta                                                                                                                     >  2:327339/1‑62 (MQ=255)

                                                                                                 cGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGtat                                                                                                                    >  1:740285/1‑61 (MQ=255)

                                                                                                 cGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGtat                                                                                                                    <  2:740285/61‑1 (MQ=255)

                                                                                                 cGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggctggc                                                                                                  <  2:707960/79‑1 (MQ=255)

                                                                                                 cGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                <  1:695069/80‑1 (MQ=17)

                                                                                                 cGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                >  2:695069/1‑80 (MQ=17)

                                                                                                  gCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAAt                                                                                      >  2:245831/1‑90 (MQ=255)

                                                                                                    aCTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCa                                   <  2:321439/139‑1 (MQ=255)

                                                                                                       atGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGa                               <  1:1468483/138‑1 (MQ=11)

                                                                                                       atGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCATTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGa                               <  2:806246/138‑1 (MQ=11)

                                                                                                        tGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAg                              <  1:716289/139‑1 (MQ=11)

                                                                                                         ggCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                >  2:1194964/1‑72 (MQ=21)

                                                                                                         ggCGACGCTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                <  1:1194964/72‑1 (MQ=18)

                                                                                                             acgcTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAAt                                                          >  1:268591/4‑106 (MQ=18)

                                                                                                             acgcTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCaa                                                    <  1:226819/109‑1 (MQ=18)

                                                                                                             acgcTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCaa                                                    >  2:226819/4‑112 (MQ=18)

                                                                                                             acgcTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCggta                         >  2:1213723/4‑136 (MQ=11)

                                                                                                              cgcTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTTGTGGTATAAATGACGGAtggctggc                                                                                                  >  1:450443/3‑65 (MQ=255)

                                                                                                              cgcTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTTGTGGTATAAATGACGGAtggctggc                                                                                                  <  2:450443/63‑1 (MQ=25)

                                                                                                              cgcTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCggtat                        >  1:1037127/3‑135 (MQ=11)

                                                                                                              cacTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTg                                                                  >  2:1156847/1‑98 (MQ=255)

                                                                                                              cacTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTg                                                                  <  1:1156847/98‑1 (MQ=255)

                                                                                                              cacTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACa                         <  2:401841/139‑1 (MQ=255)

                                                                                                                cTGGAGCGTCTTCTGAGCCTGTTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                <  2:1201633/65‑1 (MQ=37)

                                                                                                                cTGGAGCGTCTTCTGAGCCTGTTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                >  1:1201633/1‑65 (MQ=37)

                                                                                                                cTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGcc                                                                                                 <  2:403943/65‑1 (MQ=255)

                                                                                                                cTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGcc                                                                                                 >  1:403943/1‑65 (MQ=255)

                                                                                                                cTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAg                              <  2:163335/131‑1 (MQ=14)

                                                                                                                cTGGAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAg                              >  1:163335/1‑131 (MQ=14)

                                                                                                                  ggAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaat                     <  1:1444160/139‑1 (MQ=255)

                                                                                                                   gAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGta                                                                                                                     >  1:1562875/1‑41 (MQ=255)

                                                                                                                   gAGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGta                                                                                                                     <  2:1562875/41‑1 (MQ=255)

                                                                                                                    aGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCg                                                                                                                          >  1:96491/1‑36 (MQ=255)

                                                                                                                    aGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGta                                                                                                                     >  2:1631964/1‑41 (MQ=255)

                                                                                                                    aGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGta                                                                                                                     <  1:1631964/41‑1 (MQ=255)

                                                                                                                    aGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggct                                                                                                     >  2:894359/1‑57 (MQ=255)

                                                                                                                    aGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggct                                                                                                     <  1:894359/57‑1 (MQ=255)

                                                                                                                    aGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCGCGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaatct                   >  2:1096902/1‑139 (MQ=255)

                                                                                                                    aGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaatct                   >  2:219917/1‑139 (MQ=255)

                                                                                                                    aGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCgat                                               >  1:1608428/1‑108 (MQ=17)

                                                                                                                    aGCGTCTTCTGAGCCTGCTGT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCgat                                               <  2:1608428/110‑3 (MQ=17)

                                                                                                                        tcttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaatctgaat               >  2:608151/1‑139 (MQ=255)

                                                                                                                           tctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGc                                                      <  2:1478688/97‑1 (MQ=255)

                                                                                                                           tctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGc                                                      >  1:1478688/1‑97 (MQ=255)

                                                                                                                             tGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTgag          >  2:113819/1‑139 (MQ=255)

                                                                                                                              gAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTgaga         <  1:293138/139‑1 (MQ=255)

                                                                                                                                  ctgctgT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCaa                                                    <  2:323082/91‑1 (MQ=17)

                                                                                                                                  ctgctgT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCaa                                                    >  1:323082/1‑91 (MQ=17)

                                                                                                                                   tgctgT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCaa                                                    >  1:342274/1‑90 (MQ=17)

                                                                                                                                   tgctgT‑GGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCaa                                                    <  2:342274/90‑1 (MQ=17)

                                                                                                                                    gctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACAt   >  2:1192009/1‑139 (MQ=255)

                                                                                                                                     ctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATc  >  2:882708/1‑139 (MQ=255)

                                                                                                                                         cGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCaa                                                    <  2:821213/85‑1 (MQ=255)

                                                                                                                                         cGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCaa                                                    >  1:821213/1‑85 (MQ=255)

ACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATC  >  NC_000913/4518855‑4519126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`