Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	664,102	C→T	*363* (TAG→TAA)	rlpA ←	septal ring protein, suppressor of prc, minor lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	664,102	0	C	T	100.0%	191.2 / NA	59	*363* (TAG→TAA)	rlpA	septal ring protein, suppressor of prc, minor lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/28);  total (31/28)

TCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGC  >  NC_000913/663966‑664217                                                                                                                                         |                                                                                                                    tCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTa                                                                                                                   >  2:834736/1‑139 (MQ=255) tCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTa                                                                                                                   <  2:1107165/139‑1 (MQ=255)      aCTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                              <  1:688139/139‑1 (MQ=255)                                   gTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGAc                                                                                             <  1:710150/127‑1 (MQ=255)                                   gTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGAc                                                                                             >  2:710150/1‑127 (MQ=255)                                    tGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCtt                                                                                <  1:834736/139‑1 (MQ=255)                                     gCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTc                                                                               >  1:692267/1‑139 (MQ=255)                                        ctactaTAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGt                                                                            <  2:692267/139‑1 (MQ=255)                                              taGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGAc                                                                                             >  1:1560236/1‑116 (MQ=255)                                              taGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGAc                                                                                             <  2:1560236/116‑1 (MQ=255)                                                   aaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                              <  1:915991/94‑1 (MQ=255)                                                   aaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                              >  2:915991/1‑94 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                              >  2:1442184/1‑92 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                              >  2:1152718/1‑92 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                              >  2:1432643/1‑92 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                              <  1:272334/92‑1 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                              >  2:272334/1‑92 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                              <  1:1442184/92‑1 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                              <  1:1432643/92‑1 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                              <  1:1152718/92‑1 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                          <  2:1004338/96‑1 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                          >  1:1004338/1‑96 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCg                                                                              >  1:629693/1‑124 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCg                                                                              <  2:629693/124‑1 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCa                                                                       <  2:781192/131‑1 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCa                                                                       >  1:781192/1‑131 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgc                                                               >  2:1426754/1‑139 (MQ=255)                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgc                                                               >  1:1417890/1‑139 (MQ=255)                                                        aCTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGAAAACGTtgct                                                              >  1:996440/1‑137 (MQ=255)                                                        aCTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCACTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgct                                                              <  2:996440/137‑1 (MQ=255)                                                                      tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                          >  2:385188/1‑79 (MQ=255)                                                                      tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                          <  1:385188/79‑1 (MQ=255)                                                                      tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAAt                                                                                                <  1:71052/89‑1 (MQ=255)                                                                      tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAAt                                                                                                >  2:71052/1‑89 (MQ=255)                                                                             ttCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                          <  2:819088/72‑1 (MQ=255)                                                                             ttCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                          >  1:819088/1‑72 (MQ=255)                                                                              tCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGAc                                                                                             >  1:214134/1‑84 (MQ=255)                                                                              tCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGAc                                                                                             <  2:214134/84‑1 (MQ=255)                                                                                cgcgTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGAc                                                                                             <  2:1296980/82‑1 (MQ=255)                                                                                cgcgTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGAc                                                                                             >  1:1296980/1‑82 (MQ=255)                                                                                         ttGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATggg                           <  1:1426754/139‑1 (MQ=255)                                                                                                     tttACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGtaata                                                                                                   <  2:1555043/55‑1 (MQ=255)                                                                                                     tttACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGtaata                                                                                                   >  1:1555043/1‑55 (MQ=255)                                                                                                     tttACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                           <  2:1217828/95‑1 (MQ=255)                                                                                                     tttACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                           >  1:1217828/1‑95 (MQ=255)                                                                                                             aaTTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGAc       >  1:1156037/1‑139 (MQ=255)                                                                                                              aTTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGAAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGAcc      >  1:1099149/1‑139 (MQ=255)                                                                                                               ttGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACcg     >  1:615648/1‑139 (MQ=255)                                                                                                                 gACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACcgcg   >  2:271921/1‑139 (MQ=255)                                                                                                                 gACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACcgcg   <  1:271921/139‑1 (MQ=255)                                                                                                                  acacATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTa                                                      <  2:118851/87‑1 (MQ=255)                                                                                                                  acacATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTa                                                      >  1:118851/1‑87 (MQ=255)                                                                                                                  acacATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCgcgc  >  2:45189/1‑139 (MQ=255)                                                                                                                    acaTCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                           >  2:672392/1‑80 (MQ=255)                                                                                                                    acaTCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                           <  1:672392/80‑1 (MQ=255)                                                                                                                                  cTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGaa                               >  2:428079/1‑94 (MQ=255)                                                                                                                                  cTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGaa                               <  1:428079/94‑1 (MQ=255)                                                                                                                                     tACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGt                                                                  <  2:1153284/56‑1 (MQ=255)                                                                                                                                     tACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGt                                                                  >  1:1153284/1‑56 (MQ=255)                                                                                                                                         |                                                                                                                    TCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGC  >  NC_000913/663966‑664217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑