Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	672,947	C→A	V613F (GTT→TTT)	leuS ←	leucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	672,947	0	C	A	100.0%	184.8 / NA	58	V613F (GTT→TTT)	leuS	leucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/32);  total (26/32)

TTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGC  >  NC_000913/672813‑673073                                                                                                                                       |                                                                                                                               ttCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGt                                                                                                                            >  2:857064/1‑139 (MQ=255)  tCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGtt                                                                                                                           <  1:857064/139‑1 (MQ=255)    gagaGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCa                                                                                                                         <  1:899074/139‑1 (MQ=255)              tCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCg                                                                                                               <  1:69693/139‑1 (MQ=255)                  ccGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATc                                                                                                           >  1:637116/1‑139 (MQ=255)                   cGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCt                                                                                                          >  2:249878/1‑139 (MQ=255)                            aaaCATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTc                                                                                                 <  2:463487/139‑1 (MQ=255)                                   atAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATAc                                                                                          <  1:247722/139‑1 (MQ=255)                                          gACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCttt                                                                                   >  1:757401/1‑139 (MQ=255)                                           aCGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCg                                                                                                      >  1:1320432/1‑119 (MQ=255)                                           aCGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCg                                                                                                      <  2:1320432/119‑1 (MQ=255)                                           aCGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTtc                                                                                  >  1:276362/1‑139 (MQ=255)                                            cGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATgg                                                                                                                      >  2:911178/1‑102 (MQ=255)                                            cGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATgg                                                                                                                      <  1:911178/102‑1 (MQ=255)                                            cGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTtct                                                                                 >  2:801650/1‑139 (MQ=255)                                               aCGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGt                                                                              <  2:1269030/139‑1 (MQ=255)                                                 ggTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCa                                                                            <  2:74175/139‑1 (MQ=255)                                                 ggTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCa                                                                            <  2:757401/139‑1 (MQ=255)                                                 ggTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCa                                                                            <  2:881505/139‑1 (MQ=255)                                                         cgcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAAc                                                                    >  2:342343/1‑139 (MQ=255)                                                             gTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCttt                                                                                                  <  1:1022867/105‑1 (MQ=255)                                                             gTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCttt                                                                                                  >  2:1022867/1‑105 (MQ=255)                                                             gTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATa                                                                >  2:1611413/1‑139 (MQ=255)                                                                     cAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAAc                                                        <  2:1089791/139‑1 (MQ=255)                                                                      aaCCATCACCTGCGGGTCGATACCGTTGTTCTTCGTCTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACgg                                                                                        <  1:986704/106‑1 (MQ=255)                                                                      aaCCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACgg                                                                                        >  2:986704/1‑106 (MQ=255)                                                                         cATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCggg                                                    <  2:276362/139‑1 (MQ=255)                                                                            cACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCtt                                                                                                         >  2:536992/1‑83 (MQ=255)                                                                            cACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCtt                                                                                                         <  1:536992/83‑1 (MQ=255)                                                                                           aCCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCg                                  <  1:801650/139‑1 (MQ=255)                                                                                               ttgttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGt                              >  1:418851/1‑139 (MQ=255)                                                                                                 gttCTTCGACTTGTACTTTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTc                                                                       <  1:1671299/96‑1 (MQ=255)                                                                                                 gttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTc                                                                       >  2:1671299/1‑96 (MQ=255)                                                                                                 gttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCgggg                                                   >  2:648560/1‑116 (MQ=255)                                                                                                 gttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCgggg                                                   <  1:648560/116‑1 (MQ=255)                                                                                                    cttcGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGaaa                                                >  2:1444064/1‑116 (MQ=255)                                                                                                    cttcGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGaaa                                                <  1:1444064/116‑1 (MQ=255)                                                                                                     ttcGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                        <  1:699815/139‑1 (MQ=255)                                                                                                       cGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGaa                                                 >  2:1442035/1‑112 (MQ=255)                                                                                                       cGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGaa                                                 <  1:1442035/112‑1 (MQ=255)                                                                                                       cGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCa                      <  2:637116/139‑1 (MQ=255)                                                                                                       cGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCa                      >  2:419640/1‑139 (MQ=255)                                                                                                        gACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCaa                     <  1:342343/139‑1 (MQ=255)                                                                                                         aCTTGGACATTTTGCTCATGCCGGTATAAAACAGtt                                                                                                                           >  1:204805/1‑36 (MQ=255)                                                                                                         aCTTGGACATTTTGCTCATGCCGGTATAAAACAGtt                                                                                                                           <  2:204805/36‑1 (MQ=255)                                                                                                             ggACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                        >  1:700782/1‑131 (MQ=255)                                                                                                             ggACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                        <  2:700782/131‑1 (MQ=255)                                                                                                              gACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGa                                                  >  2:536567/1‑104 (MQ=255)                                                                                                              gACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGa                                                  <  1:536567/104‑1 (MQ=255)                                                                                                                       cTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCAt      >  1:933742/1‑139 (MQ=255)                                                                                                                           tGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCAt                                                            <  2:167671/81‑1 (MQ=255)                                                                                                                           tGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCAt                                                            >  1:167671/1‑81 (MQ=255)                                                                                                                           tGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGc  <  1:1611413/139‑1 (MQ=255)                                                                                                                           tGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGc  <  1:249878/139‑1 (MQ=255)                                                                                                                            gCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGt                                                                              >  1:1319384/1‑62 (MQ=255)                                                                                                                            gCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGt                                                                              <  2:1319384/62‑1 (MQ=255)                                                                                                                            gCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTc                          >  1:992596/1‑114 (MQ=255)                                                                                                                            gCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTc                          <  2:992596/114‑1 (MQ=255)                                                                                                                                       |                                                                                                                               TTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGC  >  NC_000913/672813‑673073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑