Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	789,464	G→A	I172I (ATC→ATT)	galK ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	789,464	0	G	A	100.0%	138.2 / NA	43	I172I (ATC→ATT)	galK	galactokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/19);  total (24/19)

GTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGG  >  NC_000913/789334‑789591                                                                                                                                   |                                                                                                                                gTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCa                                                                                                                         >  2:1456614/1‑139 (MQ=255)                       gacgacAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCg                                                                                                                           <  2:1311912/115‑1 (MQ=255)                       gacgacAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCg                                                                                                                           >  1:1311912/1‑115 (MQ=255)                         cgacAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATcc                                                                                                                             <  1:1324132/111‑1 (MQ=255)                         cgacAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATcc                                                                                                                             >  2:1324132/1‑111 (MQ=255)                                        tGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAAt                                                                                                                               >  2:1403746/1‑94 (MQ=255)                                            cATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGt                                                                                   >  2:421860/1‑134 (MQ=255)                                            cATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGt                                                                                   <  1:421860/134‑1 (MQ=255)                                                        tttGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTgcg                                                                  <  2:70955/139‑1 (MQ=255)                                                                 cAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGc                                                         >  2:618068/1‑139 (MQ=255)                                                                     gAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCa                                                     <  2:813034/139‑1 (MQ=255)                                                                           cAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAgatgat                                               >  2:231788/1‑139 (MQ=255)                                                                           cAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAgatgat                                               >  2:1033187/1‑139 (MQ=255)                                                                              tCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATaaa                                            >  2:864596/1‑139 (MQ=255)                                                                                         ggCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACaaa                                                                                                          >  2:1497617/1‑66 (MQ=255)                                                                                         ggCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACaaa                                                                                                          <  1:1497617/66‑1 (MQ=255)                                                                                         ggCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAAt                                 >  2:1294419/1‑139 (MQ=255)                                                                                           cATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCg                                                                                                                           <  2:639508/47‑1 (MQ=255)                                                                                           cATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCg                                                                                                                           >  1:639508/1‑47 (MQ=255)                                                                                              gATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAg                                                                                                                        <  1:142902/47‑1 (MQ=255)                                                                                              gATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAg                                                                                                                        >  2:142902/1‑47 (MQ=255)                                                                                              gATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAgc                                          <  2:1331949/125‑1 (MQ=255)                                                                                              gATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAgc                                          >  1:1331949/1‑125 (MQ=255)                                                                                              gATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGt                            >  1:760199/1‑139 (MQ=255)                                                                                                    tcttGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAg                                                          >  2:144877/1‑103 (MQ=255)                                                                                                    tcttGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAg                                                          <  1:144877/103‑1 (MQ=255)                                                                                                    tcttGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCa                                                     <  1:110426/108‑1 (MQ=255)                                                                                                    tcttGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCa                                                     >  2:110426/1‑108 (MQ=255)                                                                                                        gCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACcgcg                  <  1:618068/139‑1 (MQ=255)                                                                                                        gCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACcgcg                  <  1:231788/139‑1 (MQ=255)                                                                                                        gCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACcgcg                  <  1:864596/139‑1 (MQ=255)                                                                                                            agtgcgGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACtt              >  1:215598/4‑139 (MQ=255)                                                                                                            aGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCg                                                        >  2:792909/1‑97 (MQ=255)                                                                                                            aGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCg                                                        <  1:792909/97‑1 (MQ=255)                                                                                                            aGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACtt              >  1:857996/1‑139 (MQ=255)                                                                                                              cgcgGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGc                                                                                              <  2:474666/57‑1 (MQ=255)                                                                                                              cgcgGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGc                                                                                              >  1:474666/1‑57 (MQ=255)                                                                                                              cgcgGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGt                                                                                   >  2:962103/1‑68 (MQ=255)                                                                                                              cgcgGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGt                                                                                   <  1:962103/68‑1 (MQ=255)                                                                                                              cgcgGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACcgcg                  <  2:58475/133‑1 (MQ=255)                                                                                                              cgcgGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACcgcg                  >  1:58475/1‑133 (MQ=255)                                                                                                              cgcgGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTcc            >  2:1190475/1‑139 (MQ=255)                                                                                                                        aGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCgg  <  1:1294419/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                GTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGG  >  NC_000913/789334‑789591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑