Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	872,801	G→A	*304* (TAG→TAA)	gsiD →	glutathione ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	872,801	0	G	A	100.0%	211.9 / NA	66	*304* (TAG→TAA)	gsiD	glutathione ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/33);  total (33/33)

CTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTG  >  NC_000913/872671‑872934                                                                                                                                   |                                                                                                                                      cTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGttt                                                                                                                               >  2:751466/1‑139 (MQ=255)        ggATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATAtt                                                                                                                        <  1:355747/139‑1 (MQ=255)                  aTCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAgg                                                                                                              <  2:943433/139‑1 (MQ=255)                   tCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGTTAATTACGTTTGAATATTGCTTGAAAggg                                                                                                             >  2:932850/1‑139 (MQ=255)                    cgcgCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTa                                                                                                                                    <  2:805539/115‑1 (MQ=255)                    cgcgCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTa                                                                                                                                    >  1:805539/1‑115 (MQ=255)                    cgcgCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGt                                                                                                            >  1:944275/1‑139 (MQ=255)                                  cTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAgg                                                                                              <  1:751466/139‑1 (MQ=255)                                        ttCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAatta                                                                                        <  2:1170488/139‑1 (MQ=255)                                          ccGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGaaa                                                                                                                <  2:1589571/113‑1 (MQ=255)                                          ccGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGaaa                                                                                                                >  1:1589571/1‑113 (MQ=255)                                          ccGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAgg                                                                                                              <  2:143328/115‑1 (MQ=255)                                          ccGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAgg                                                                                                              >  1:143328/1‑115 (MQ=255)                                                           tCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGTTAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGtgttg                                                                     <  1:932850/139‑1 (MQ=255)                                                              gACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCCCAGGAAATTATTGcc                                                                                   >  2:376634/1‑122 (MQ=255)                                                              gACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGcc                                                                                   <  1:376634/122‑1 (MQ=255)                                                                 cGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAata                                                                                                                          >  2:618281/1‑80 (MQ=255)                                                                 cGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAata                                                                                                                          <  1:618281/80‑1 (MQ=255)                                                                   tACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTcaca                                                                                                <  2:280941/104‑1 (MQ=255)                                                                   tACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTcaca                                                                                                >  1:280941/1‑104 (MQ=255)                                                                    aCTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGaa                                                                                                                 >  2:1434650/1‑86 (MQ=255)                                                                    aCTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGaa                                                                                                                 <  1:1434650/86‑1 (MQ=255)                                                                    aCTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGa                                                                                                                  >  1:768239/1‑85 (MQ=255)                                                                    aCTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGa                                                                                                                  <  2:768239/85‑1 (MQ=255)                                                                         cGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATAtt                                                                                                                        <  1:770061/74‑1 (MQ=255)                                                                         cGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATAtt                                                                                                                        >  2:770061/1‑74 (MQ=255)                                                                         cGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCt                                                                                                                     <  1:1014453/77‑1 (MQ=255)                                                                         cGTTCAATTTGTTGAGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATACTGCt                                                                                                                     >  2:1014453/1‑77 (MQ=255)                                                                           ttCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCAc                                                                                                      <  1:537359/90‑1 (MQ=255)                                                                           ttCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCAc                                                                                                      >  2:537359/1‑90 (MQ=255)                                                                           ttCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCAc                                                                                                      <  2:1594040/90‑1 (MQ=255)                                                                           ttCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCAc                                                                                                      >  1:1594040/1‑90 (MQ=255)                                                                           ttCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTctgc                                                          <  2:86382/134‑1 (MQ=255)                                                                           ttCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTctgc                                                          >  1:86382/1‑134 (MQ=255)                                                                           ttCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGAta                                                     >  1:1550412/1‑138 (MQ=255)                                                                             cAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGccc                                                                                  <  2:872611/108‑1 (MQ=255)                                                                             cAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGccc                                                                                  >  1:872611/1‑108 (MQ=255)                                                                             cAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGAtttt                                                   >  2:1262402/1‑139 (MQ=255)                                                                             cAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGAtttt                                                   >  1:1285911/1‑139 (MQ=255)                                                                             cAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGAtttt                                                   >  2:5944/1‑139 (MQ=255)                                                                              aaTTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTctgc                                                          <  1:1376153/131‑1 (MQ=255)                                                                              aaTTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTctgc                                                          >  2:1376153/1‑131 (MQ=255)                                                                                  tgttgGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTg                                                                                                                   >  2:807384/1‑70 (MQ=255)                                                                                  tgttgGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTg                                                                                                                   <  1:807384/70‑1 (MQ=255)                                                                                  tgttgGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGa                                              <  1:1262402/139‑1 (MQ=255)                                                                                  tgttgGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGa                                              >  2:466733/1‑139 (MQ=255)                                                                                   gttgGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGaa                                             <  2:944275/139‑1 (MQ=255)                                                                                       ggCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCgg                                         <  1:466733/139‑1 (MQ=255)                                                                                        gCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCt                                                                                                    <  1:1208195/79‑1 (MQ=255)                                                                                        gCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCt                                                                                                    >  2:1208195/1‑79 (MQ=255)                                                                                              gTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAAttt                                  <  2:1661969/139‑1 (MQ=255)                                                                                                        tGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGaa                                                                                                                            >  1:193341/1‑39 (MQ=255)                                                                                                        tGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGaa                                                                                                                            <  2:193341/39‑1 (MQ=255)                                                                                                        tGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAg                                                                          <  1:929202/89‑1 (MQ=255)                                                                                                        tGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAg                                                                          >  2:929202/1‑89 (MQ=255)                                                                                                        tGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTctgc                                                          >  2:1282988/1‑105 (MQ=255)                                                                                                        tGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTctgc                                                          <  1:1282988/105‑1 (MQ=255)                                                                                                        tGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGAt                                                      >  1:89911/1‑109 (MQ=255)                                                                                                        tGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGAt                                                      <  2:89911/109‑1 (MQ=255)                                                                                                        tGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAg                        >  1:781407/1‑139 (MQ=255)                                                                                                        tGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAg                        >  2:820248/1‑139 (MQ=255)                                                                                                                  ccGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTctgc                                                          >  2:549922/1‑95 (MQ=255)                                                                                                                  ccGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTctgc                                                          <  1:549922/95‑1 (MQ=255)                                                                                                                          tAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAggg            <  2:1568993/133‑1 (MQ=255)                                                                                                                          tAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAggg            >  1:1568993/1‑133 (MQ=255)                                                                                                                              ggATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTg  <  2:165583/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                      CTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTG  >  NC_000913/872671‑872934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑