Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 913,349 G→A L156L (CTG→TTG)  hcp ← hybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913913,3490GA98.8% 257.8 / ‑5.5 81L156L (CTG→TTG) hcphybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (41/39);  minor base T (0/1);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAT  >  NC_000913/913220‑913472
                                                                                                                                 |                                                                                                                           
cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCAcagcag                                                                                                                    >  1:41596/1‑139 (MQ=255)
                            ccACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATc                                                                                        <  2:1391039/139‑1 (MQ=255)
                            ccACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATc                                                                                        <  1:692235/139‑1 (MQ=255)
                               cGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGc                                                                                                                           >  1:427298/1‑101 (MQ=255)
                               cGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGc                                                                                                                           <  2:427298/101‑1 (MQ=255)
                                gCCATGATTTTATGGTACTGGGCCTAAATATCGTTGTCGTATTGACCGAGAACATGCGCG‑GTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgccg                                                                                    <  2:933563/138‑1 (MQ=255)
                                gCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCG‑GTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgccg                                                                                    >  1:933563/1‑138 (MQ=255)
                                                aCTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAgg                                                                    >  2:470559/1‑139 (MQ=255)
                                                 cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAggg                                                                   <  1:470559/139‑1 (MQ=255)
                                                    ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                                  <  1:1035157/121‑1 (MQ=255)
                                                    ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                                  >  2:1035157/1‑121 (MQ=255)
                                                         aaaTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTc                                                           >  1:108139/1‑139 (MQ=255)
                                                         aaaTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTc                                                           >  1:1325797/1‑139 (MQ=255)
                                                              tCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCAcag                                                                                                                       >  2:222105/1‑74 (MQ=255)
                                                              tCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCAcag                                                                                                                       <  1:222105/74‑1 (MQ=255)
                                                                gTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGcc                                                    <  2:41596/139‑1 (MQ=255)
                                                                   gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGca                                                                                                                          <  1:1399009/66‑1 (MQ=255)
                                                                   gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGca                                                                                                                          >  2:1399009/1‑66 (MQ=255)
                                                                   gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGa                                                 >  2:1492561/1‑139 (MQ=255)
                                                                   gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGa                                                 >  2:891701/1‑139 (MQ=255)
                                                                    tCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATc                                                                                        <  2:1506427/99‑1 (MQ=255)
                                                                    tCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATc                                                                                        >  1:1506427/1‑99 (MQ=255)
                                                                          tGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGATGTTGCa                                          <  1:1492561/139‑1 (MQ=255)
                                                                            aCCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCTATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCTATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGc                                        >  2:1065191/1‑139 (MQ=255)
                                                                            aCCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGc                                        >  1:376584/1‑139 (MQ=255)
                                                                            aCCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTCTCAGGCCATACAAGCACAGCAGACg                                                                                                                 <  1:69497/66‑1 (MQ=255)
                                                                            aCCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTCTCAGGCCATACAAGCACAGCAGACg                                                                                                                 >  2:69497/1‑66 (MQ=255)
                                                                              cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAggg                                                                   <  1:1105307/110‑1 (MQ=255)
                                                                              cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAggg                                                                   >  2:1105307/1‑110 (MQ=255)
                                                                              cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg                                                         <  1:393269/120‑1 (MQ=255)
                                                                              cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg                                                         >  2:393269/1‑120 (MQ=255)
                                                                              cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCt                                                   <  1:697609/126‑1 (MQ=255)
                                                                              cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCt                                                   >  2:697609/1‑126 (MQ=255)
                                                                                 gaACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGcc                                   >  2:213764/1‑139 (MQ=255)
                                                                                      tGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcg                                                                                       <  1:391721/82‑1 (MQ=255)
                                                                                      tGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcg                                                                                       >  2:391721/1‑82 (MQ=255)
                                                                                      tGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAt                              >  1:590115/1‑139 (MQ=255)
                                                                                       gcgcGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAtc                             >  1:880917/1‑139 (MQ=255)
                                                                                        cgcgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAtcg                            <  1:891701/139‑1 (MQ=255)
                                                                                            tgtTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGc                        <  2:590115/139‑1 (MQ=255)
                                                                                              ttCCATATAGGCCGCGTCACCTTTCAGGCCATACATGCACCGCAGACGCAGGCCGAGAAGTTTTACGCCAAGCTCCGCTTTATCTTTGTGAGGTGTACATTCTGGTGCCTGACTTTGCAGCTCGCCGAGATCGTCGCTc                      <  1:1645146/139‑1 (MQ=255)
                                                                                                ccATATAGGCCGCCGCACCTTTCAGGTCATACAAGCATAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAc                    <  1:1065191/139‑1 (MQ=255)
                                                                                                ccATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAc                    <  1:526087/139‑1 (MQ=255)
                                                                                                ccATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAc                    <  2:108139/139‑1 (MQ=255)
                                                                                                      aGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                                  <  1:900328/71‑1 (MQ=255)
                                                                                                      aGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCt                                                                                  >  2:900328/1‑71 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTa                                                                      >  2:574737/1‑81 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTa                                                                      <  1:574737/81‑1 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCa            >  1:813616/1‑139 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCa            >  1:1506133/1‑139 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCa            >  1:283481/1‑139 (MQ=255)
                                                                                                         ccgccgCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGc                                                                                                            <  2:701694/42‑1 (MQ=255)
                                                                                                         ccgccgCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGc                                                                                                            >  1:701694/1‑42 (MQ=255)
                                                                                                          cgccgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAgg          >  2:1530456/1‑139 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCttt                                                                                <  1:248859/66‑1 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCttt                                                                                >  2:248859/1‑66 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATc                                                                             >  2:733045/1‑69 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATc                                                                             <  1:733045/69‑1 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTa                                                                      >  2:1249042/1‑76 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTa                                                                      <  1:1249042/76‑1 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                               >  1:591819/1‑83 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                               <  2:591819/83‑1 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTct                                                          >  1:129568/1‑88 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTct                                                          <  2:129568/88‑1 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTct                                                          >  1:1506099/1‑88 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTct                                                          <  2:1506099/88‑1 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCa       >  2:937345/1‑139 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCa       >  2:1603121/1‑139 (MQ=255)
                                                                                                                  cTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt  <  1:1530456/139‑1 (MQ=255)
                                                                                                                  cTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt  <  2:1506133/139‑1 (MQ=255)
                                                                                                                  cTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt  <  1:664021/139‑1 (MQ=255)
                                                                                                                  cTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt  <  2:376584/139‑1 (MQ=255)
                                                                                                                           cATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgccg                                                                                    >  1:1516646/1‑48 (MQ=255)
                                                                                                                           cATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgccg                                                                                    <  2:1516646/48‑1 (MQ=255)
                                                                                                                            aTACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg                                                         >  2:1248411/1‑74 (MQ=255)
                                                                                                                            aTACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg                                                         <  1:1248411/74‑1 (MQ=255)
                                                                                                                            aTACAAACACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAggg                                                                   >  1:1667772/1‑64 (MQ=255)
                                                                                                                            aTACAAACACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAggg                                                                   <  2:1667772/64‑1 (MQ=255)
                                                                                                                              aCAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAg                                         <  2:1414104/88‑1 (MQ=255)
                                                                                                                              aCAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt  <  1:1641590/127‑1 (MQ=255)
                                                                                                                              aCAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt  >  2:1641590/1‑127 (MQ=255)
                                                                                                                                 |                                                                                                                           
CGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAT  >  NC_000913/913220‑913472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑