Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 88,325 G→A E100K (GAA→AAA)  cra → transcriptional repressor‑activator for carbon metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091388,3250GA100.0% 282.9 / NA 86E100K (GAA→AAA) cratranscriptional repressor‑activator for carbon metabolism
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/41);  total (45/41)

GGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTTCCCGAT  >  NC_000913/88190‑88458
                                                                                                                                       |                                                                                                                                     
gggCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCaaaag                                                                                                                                    >  2:1445258/1‑139 (MQ=255)
      cGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGc                                                                                                                              >  1:1025476/1‑139 (MQ=255)
        tGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCa                                                                                                                            >  1:534403/1‑139 (MQ=255)
            ggACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACa                                                                                                                        >  1:808589/1‑139 (MQ=255)
                 cacaCGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTCTCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                   <  2:1025476/139‑1 (MQ=255)
                               gTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                     <  2:808589/139‑1 (MQ=255)
                               gTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                     <  2:1547745/139‑1 (MQ=255)
                                 cTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGc                                                                                                   >  1:275276/1‑139 (MQ=255)
                                  ttGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCg                                                                                                              >  2:70899/1‑127 (MQ=255)
                                  ttGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCg                                                                                                              <  1:70899/127‑1 (MQ=255)
                                  ttGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCa                                                                                                  >  2:1249057/1‑139 (MQ=255)
                                    gtgATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCAcc                                                                                                >  2:245450/1‑139 (MQ=255)
                                         ccccGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGTCAACGaa                                                                                                                   <  1:1095225/115‑1 (MQ=255)
                                         ccccGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                   >  2:1095225/1‑115 (MQ=255)
                                          cccGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTAc                                                                                          >  1:1333054/1‑139 (MQ=255)
                                                    agaACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTc                                                                                    >  2:98178/1‑135 (MQ=255)
                                                    agaACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTc                                                                                    <  1:98178/135‑1 (MQ=255)
                                                       acacCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGa                                                                             <  1:245450/139‑1 (MQ=255)
                                                        cacCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                     <  2:1336164/114‑1 (MQ=255)
                                                        cacCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                     >  1:1336164/1‑114 (MQ=255)
                                                         acCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                   >  1:1655586/1‑99 (MQ=255)
                                                         acCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                   <  2:1655586/99‑1 (MQ=255)
                                                         acCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGt                                                                                                            >  1:869325/1‑106 (MQ=255)
                                                         acCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGt                                                                                                            <  2:869325/106‑1 (MQ=255)
                                                         acCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATg                                                                           >  1:368546/1‑139 (MQ=255)
                                                           cAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                   >  2:299025/1‑97 (MQ=255)
                                                           cAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                   <  1:299025/97‑1 (MQ=255)
                                                             gCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTa                                                                                           >  2:18339/1‑119 (MQ=255)
                                                             gCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTa                                                                                           <  1:18339/119‑1 (MQ=255)
                                                                   cccGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                   <  1:122088/89‑1 (MQ=255)
                                                                   cccGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                   >  2:122088/1‑89 (MQ=255)
                                                                   cccGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                   >  2:14403/1‑89 (MQ=255)
                                                                   cccGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                   <  1:14403/89‑1 (MQ=255)
                                                                       cATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGCAATg                                                                                                                >  2:887260/1‑88 (MQ=255)
                                                                       cATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATg                                                                                                                <  1:887260/88‑1 (MQ=255)
                                                                        aTCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                     <  2:1299896/98‑1 (MQ=255)
                                                                        aTCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                     >  1:1299896/1‑98 (MQ=255)
                                                                        aTCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTa                                                                                           <  2:609324/108‑1 (MQ=255)
                                                                        aTCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTa                                                                                           >  1:609324/1‑108 (MQ=255)
                                                                        aTCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGc                                                                          >  1:1599087/1‑125 (MQ=255)
                                                                        aTCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGc                                                                          <  2:1599087/125‑1 (MQ=255)
                                                                           gCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTa                                                                                           <  1:139925/105‑1 (MQ=255)
                                                                           gCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTa                                                                                           >  2:139925/1‑105 (MQ=255)
                                                                                cTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGa                                                                             <  1:735458/114‑1 (MQ=255)
                                                                                cTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGa                                                                             >  2:735458/1‑114 (MQ=255)
                                                                                 tATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGc                                                   <  2:1333054/139‑1 (MQ=255)
                                                                                  aTCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                     <  1:1548604/88‑1 (MQ=255)
                                                                                  aTCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                     >  2:1548604/1‑88 (MQ=255)
                                                                                    cTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGa                                                            <  2:1459947/127‑1 (MQ=255)
                                                                                    cTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGa                                                            >  1:1459947/1‑127 (MQ=255)
                                                                                    cTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGcctc                                                >  1:544934/1‑139 (MQ=255)
                                                                                     ttGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                   >  2:93061/1‑71 (MQ=255)
                                                                                     ttGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                   <  1:93061/71‑1 (MQ=255)
                                                                                      tGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGcctcct                                              <  2:368546/139‑1 (MQ=255)
                                                                                          cGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAg                                                                                                    >  1:128858/1‑81 (MQ=255)
                                                                                          cGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAg                                                                                                    <  2:128858/81‑1 (MQ=255)
                                                                                          cGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAg                                                                                                    <  2:1394240/81‑1 (MQ=255)
                                                                                          cGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAg                                                                                                    >  1:1394240/1‑81 (MQ=255)
                                                                                           gCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                     >  2:982635/1‑79 (MQ=255)
                                                                                           gCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                     <  1:982635/79‑1 (MQ=255)
                                                                                           gCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCa                                         <  1:1445258/139‑1 (MQ=255)
                                                                                           gCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCATGAGCa                                         <  1:1249057/139‑1 (MQ=255)
                                                                                             cAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATc                                       >  2:119499/1‑139 (MQ=255)
                                                                                             cAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATc                                       >  2:388326/1‑139 (MQ=255)
                                                                                               ggCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGAcgtcgt                                                      >  1:591925/1‑122 (MQ=255)
                                                                                               ggCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGAcgtcgt                                                      <  2:591925/122‑1 (MQ=255)
                                                                                                     gCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAAt                                                                                                                 >  1:1104937/1‑57 (MQ=255)
                                                                                                     gCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAAt                                                                                                                 <  2:1104937/57‑1 (MQ=255)
                                                                                                     gCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTAt                               >  1:421419/1‑139 (MQ=255)
                                                                                                       aaCGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGc                                                                          <  1:677717/94‑1 (MQ=255)
                                                                                                       aaCGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGc                                                                          >  2:677717/1‑94 (MQ=255)
                                                                                                          ggggTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACg                          <  1:388326/139‑1 (MQ=255)
                                                                                                                 tCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGc                                                                                                          >  2:1217147/1‑52 (MQ=255)
                                                                                                                 tCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGc                                                                                                          <  1:1217147/52‑1 (MQ=255)
                                                                                                                   aaCTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTaa                 <  1:784294/139‑1 (MQ=255)
                                                                                                                      tgctgATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGcctc                                                <  2:280833/105‑1 (MQ=255)
                                                                                                                      tgctgATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGcctc                                                >  1:280833/1‑105 (MQ=255)
                                                                                                                        ctgATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGAcc            >  2:1313639/1‑139 (MQ=255)
                                                                                                                           aTTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACg                          <  1:1409466/122‑1 (MQ=255)
                                                                                                                           aTTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACg                          >  2:1409466/1‑122 (MQ=255)
                                                                                                                                cTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACa                                                                                         <  2:430078/54‑1 (MQ=255)
                                                                                                                                cTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACa                                                                                         >  1:430078/1‑54 (MQ=255)
                                                                                                                                cTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTCCGACGTCGTTGCCTCCTGAGCATCCTTTTTAt                               <  1:808086/112‑1 (MQ=255)
                                                                                                                                cTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTCCGACGTCGTTGCCTCCTGAGCATCCTTTTTAt                               >  2:808086/1‑112 (MQ=255)
                                                                                                                                  gCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTa                  >  2:242091/1‑123 (MQ=255)
                                                                                                                                  gCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTa                  <  1:242091/123‑1 (MQ=255)
                                                                                                                                  gCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTTCCCGAt  >  1:1579948/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                     
GGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTTCCCGAT  >  NC_000913/88190‑88458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑