Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	966,584	G→A	*755* (TAG→TAA)	ycaI →	ComEC family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	966,584	0	G	A	100.0%	131.3 / NA	41	*755* (TAG→TAA)	ycaI	ComEC family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/20);  total (21/20)

TGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTA  >  NC_000913/966450‑966716                                                                                                                                       |                                                                                                                                     tGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAata                                                                                                                                  >  1:1278020/1‑139 (MQ=255)      tGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCgg                                                                                                                             <  2:1278020/139‑1 (MQ=255)      tGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCgg                                                                                                                             <  1:1478817/139‑1 (MQ=255)                caTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCAGCTATTTcaac                                                                                                                   >  2:1398193/1‑139 (MQ=255)                        tCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTg                                                                                                           >  1:1505263/1‑139 (MQ=255)                                 ggTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGt                                                                                                         >  1:852825/1‑132 (MQ=255)                                 ggTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGt                                                                                                         <  2:852825/132‑1 (MQ=255)                                      aTTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCa                                                                                             <  2:1505263/139‑1 (MQ=255)                                                gcAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCAGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACaaa                                                                                   <  1:1398193/139‑1 (MQ=255)                                                 cAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGa                                                                                                  >  2:667923/1‑123 (MQ=255)                                                 cAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGa                                                                                                  <  1:667923/123‑1 (MQ=255)                                                         gCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATctctct                                                                          <  2:664917/139‑1 (MQ=255)                                                               tAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTgg                                                                    >  1:127344/1‑139 (MQ=255)                                                                agcagcCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGc                                                                   >  2:626807/1‑139 (MQ=255)                                                                         aGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAatat                                                                                                                                 >  2:1565871/1‑68 (MQ=255)                                                                         aGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAatat                                                                                                                                 <  1:1565871/68‑1 (MQ=255)                                                                         aGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTcc                                                          >  1:1214555/1‑139 (MQ=255)                                                                           ggAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTccgc                                                        <  2:127344/139‑1 (MQ=255)                                                                                                      tcaGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                 <  2:520754/71‑1 (MQ=255)                                                                                                      tcaGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                 >  1:520754/1‑71 (MQ=255)                                                                                                        aGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCa                                                                                             <  1:1107030/73‑1 (MQ=255)                                                                                                        aGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCa                                                                                             >  2:1107030/1‑73 (MQ=255)                                                                                                        aGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATa                                                                                           <  2:184764/75‑1 (MQ=255)                                                                                                        aGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAAAAAATGGTGGTTTTTTGAATGCATa                                                                                           >  1:184764/1‑75 (MQ=255)                                                                                                          tGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACaaa                                                                                                                >  1:390241/1‑52 (MQ=255)                                                                                                          tGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACaaa                                                                                                                <  2:390241/52‑1 (MQ=255)                                                                                                          tGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCa                                                                                             >  1:764396/1‑71 (MQ=255)                                                                                                          tGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCa                                                                                             <  2:764396/71‑1 (MQ=255)                                                                                                          tGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                         <  1:1561503/139‑1 (MQ=255)                                                                                                               tGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATa                                                                                           >  2:193399/1‑68 (MQ=255)                                                                                                               tGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATa                                                                                           <  1:193399/68‑1 (MQ=255)                                                                                                                  cGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGAc                                                    <  1:1254770/104‑1 (MQ=255)                                                                                                                  cGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGAc                                                    >  2:1254770/1‑104 (MQ=255)                                                                                                                  cGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTg                 >  1:157864/1‑139 (MQ=255)                                                                                                                            gATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGAc                                                                                      >  1:1284674/1‑60 (MQ=255)                                                                                                                            gATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGAc                                                                                      <  2:1284674/60‑1 (MQ=255)                                                                                                                                 cGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTccgc                                                        <  2:1202774/85‑1 (MQ=255)                                                                                                                                 cGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTccgc                                                        >  1:1202774/1‑85 (MQ=255)                                                                                                                                 cGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCggg                     >  2:546044/1‑120 (MQ=255)                                                                                                                                 cGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCggg                     <  1:546044/120‑1 (MQ=255)                                                                                                                                 cGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTa  >  2:762831/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                     TGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTA  >  NC_000913/966450‑966716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑