Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	111,433	G→A	*130* (TAG→TAA)	mutT →	dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	111,433	0	G	A	100.0%	159.0 / NA	49	*130* (TAG→TAA)	mutT	dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/26);  total (23/26)

GTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGTCGGATAA  >  NC_000913/111302‑111558                                                                                                                                    |                                                                                                                              gTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGAt                                                                                                                        >  1:1016613/1‑139 (MQ=255)   cGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATaa                                                                                                                      <  2:142298/139‑1 (MQ=255)              aaGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTcgc                                                                                                           <  2:1032178/139‑1 (MQ=255)                   gAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTcgccg                                                                                                         <  2:1230906/136‑1 (MQ=255)                   gAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTcgccg                                                                                                         >  1:1230906/1‑136 (MQ=255)                   gAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCAt                                                                                                      >  1:1363982/1‑139 (MQ=255)                    aGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGtt                                                                                                                <  1:1135679/128‑1 (MQ=255)                    aGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGtt                                                                                                                >  2:1135679/1‑128 (MQ=255)                      ccGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCg                                                                                                   <  2:1363982/139‑1 (MQ=255)                      ccGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAATCAGATAAGGCGTTTTCGCCGCATCCg                                                                                                   >  1:736625/1‑139 (MQ=255)                       cGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTcgc                                                                                                           <  1:1064777/130‑1 (MQ=255)                       cGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTcgc                                                                                                           >  2:1064777/1‑130 (MQ=255)                             gTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCg                                                                                            <  1:1021120/139‑1 (MQ=255)                             gTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCg                                                                                            <  2:736625/139‑1 (MQ=255)                              tAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGc                                                                                           <  1:889808/139‑1 (MQ=255)                               aaaGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGca                                                                                          <  2:203593/139‑1 (MQ=255)                                      ggCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATg                                                                                   >  2:610089/1‑139 (MQ=255)                                          aCCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTg                                                                               >  1:989695/1‑139 (MQ=255)                                            ccGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCTATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGAt                                                                             >  1:1329550/1‑139 (MQ=255)                                                      gATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTgg                                                                   <  2:1599397/139‑1 (MQ=255)                                                        tGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGgcg                                                                 >  2:1410108/1‑139 (MQ=255)                                                                gtcggtcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTAt                                                         <  1:1410108/139‑1 (MQ=255)                                                                   ggtcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                      <  1:570216/139‑1 (MQ=255)                                                                     tcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATaa                                                                                                                      <  1:482029/73‑1 (MQ=255)                                                                     tcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATaa                                                                                                                      >  2:482029/1‑73 (MQ=255)                                                                     tcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCa                                                                                                       <  1:424683/88‑1 (MQ=255)                                                                     tcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCa                                                                                                       >  2:424683/1‑88 (MQ=255)                                                                           tGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAg                                              <  1:1188359/139‑1 (MQ=255)                                                                            gCCGATGATTTTCCGCCAGCCTATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAgg                                             <  2:1329550/139‑1 (MQ=255)                                                                                atgatTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGAtt                                         >  1:208109/1‑139 (MQ=255)                                                                                        ccGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACg                                                                                      <  1:1041527/86‑1 (MQ=255)                                                                                        ccGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACg                                                                                      >  2:1041527/1‑86 (MQ=255)                                                                                               ccaATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGAt                                                                                    <  2:1145525/81‑1 (MQ=255)                                                                                               ccaATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGAt                                                                                    >  1:1145525/1‑81 (MQ=255)                                                                                                        cGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTgg                                                                   >  1:1395288/1‑89 (MQ=255)                                                                                                        cGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTgg                                                                   <  2:1395288/89‑1 (MQ=255)                                                                                                         ggTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaaa                                               >  1:1034549/1‑108 (MQ=255)                                                                                                         ggTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaaa                                               <  2:1034549/108‑1 (MQ=255)                                                                                                         ggTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGtttt                >  2:1490443/1‑139 (MQ=255)                                                                                                          gTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAg                                              <  2:401150/108‑1 (MQ=255)                                                                                                          gTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAg                                              >  1:401150/1‑108 (MQ=255)                                                                                                          gTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGttttt               >  1:41267/1‑139 (MQ=255)                                                                                                           tAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATcc                                                                                                    >  1:572591/1‑53 (MQ=255)                                                                                                           tAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATcc                                                                                                    <  2:572591/53‑1 (MQ=255)                                                                                                              ttGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAg           <  1:786267/139‑1 (MQ=255)                                                                                                                                tGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTgg                                                                   <  1:69946/65‑1 (MQ=255)                                                                                                                                tGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTgg                                                                   >  2:69946/1‑65 (MQ=255)                                                                                                                                tGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGTCGGATaa  <  2:122665/130‑1 (MQ=255)                                                                                                                                tGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGTCGGATaa  >  1:122665/1‑130 (MQ=255)                                                                                                                                    |                                                                                                                              GTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGTCGGATAA  >  NC_000913/111302‑111558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑