Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,149,712	G→A	*318* (TAG→TAA)	fabH →	3‑oxoacyl‑[acyl‑carrier‑protein] synthase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,149,712	0	G	A	100.0%	136.9 / NA	43	*318* (TAG→TAA)	fabH	3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/24);  total (19/24)

AATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCA  >  NC_000913/1149578‑1149808                                                                                                                                       |                                                                                                  aaTACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATa                                                                                               <  1:867141/139‑1 (MQ=255)   tACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAg                                                                                             <  2:261581/139‑1 (MQ=255)              ccTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACAt                                                                                  >  2:1450303/1‑139 (MQ=255)                    tCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCa                                                                            <  1:1359355/139‑1 (MQ=255)                         tGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTg                                                                       <  1:1450303/139‑1 (MQ=255)                           cgcgcTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCa                                                                     >  2:400991/1‑139 (MQ=255)                              gcTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCAttt                                                                  >  1:1475256/1‑139 (MQ=255)                                tGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                <  2:1667652/139‑1 (MQ=255)                                            tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                >  2:991709/1‑127 (MQ=255)                                            tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTtgt                                                                <  1:991709/127‑1 (MQ=255)                                            tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACa                                                    >  1:1101311/1‑139 (MQ=255)                                            tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACa                                                    >  2:1303190/1‑139 (MQ=255)                                             aCGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑g                                                  <  2:1101311/139‑1 (MQ=255)                                              cgcgACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGAt                                                                                          >  2:1613525/1‑99 (MQ=255)                                              cgcgACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGAt                                                                                          <  1:1613525/99‑1 (MQ=255)                                               gcgACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑ggg                                                <  1:400991/139‑1 (MQ=255)                                                        gcATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAAc                                       <  1:1303190/139‑1 (MQ=255)                                                           ttAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGt                                    >  1:1015152/1‑139 (MQ=255)                                                             aaGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGTTg                                  >  2:1553187/1‑139 (MQ=255)                                                             aaGCCGGGGCAGTTGGTTCTACTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGTTg                                  >  1:1019567/1‑139 (MQ=255)                                                                  ggggCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGTTGGAATg                             <  2:1638198/139‑1 (MQ=255)                                                                       aGTTGGTTCTACTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGTTGGAATgctggc                        <  2:1019567/139‑1 (MQ=255)                                                                         ttGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAAc                                       >  2:1094357/1‑122 (MQ=255)                                                                         ttGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTATAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTCCTCAAAc                                       <  1:1094357/122‑1 (MQ=255)                                                                                 gCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGTTGGAATGCTGGCTGATATggcg              <  1:1553187/139‑1 (MQ=255)                                                                                 gCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGTTGGAATGCTGGCTGATATggcg              <  2:1015152/139‑1 (MQ=255)                                                                                  cTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGTTAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGa                                                      <  1:698759/99‑1 (MQ=255)                                                                                  cTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGTTAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGa                                                      >  2:698759/1‑99 (MQ=255)                                                                                   ttGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGTTGGAATGCTGGCTGATATggcggc            <  1:627376/139‑1 (MQ=255)                                                                                     gAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGtt                                   >  1:1667499/1‑114 (MQ=255)                                                                                     gAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGtt                                   <  2:1667499/114‑1 (MQ=255)                                                                                       aGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCT‑GTTCGTTTCTTAGATAAGGGTTAAAACATTGAGCAATTTTCATTTTTGTTTCCTGGACAGGGGTTCT‑AAACCGTTTGGATGCTTGCTTATATTGCGGGGAggt       >  2:956837/1‑137 (MQ=255)                                                                                             tGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCa  <  2:1475256/139‑1 (MQ=255)                                                                                                               gggCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCa                                                                            >  2:1636736/1‑48 (MQ=255)                                                                                                               gggCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCa                                                                            <  1:1636736/48‑1 (MQ=255)                                                                                                               gggCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGTTGGAATGCTGGCTGATATggcg              >  2:514860/1‑109 (MQ=255)                                                                                                               gggCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGTTGGAATGCTGGCTGATATggcg              <  1:514860/109‑1 (MQ=255)                                                                                                                ggCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACg                                                                              <  1:403150/45‑1 (MQ=255)                                                                                                                ggCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACg                                                                              >  2:403150/1‑45 (MQ=255)                                                                                                                ggCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCAt                                                                    >  1:396935/1‑55 (MQ=255)                                                                                                                ggCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCAt                                                                    <  2:396935/55‑1 (MQ=255)                                                                                                                ggCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCaaa                                        >  2:564216/1‑82 (MQ=255)                                                                                                                ggCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCaaa                                        <  1:564216/82‑1 (MQ=255)                                                                                                                                       |                                                                                                  AATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACA‑GGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCA  >  NC_000913/1149578‑1149808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑