Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,205,731	G→A	*61* (TAG→TAA)  pseudogene (1/412 nt)	ymfR → beeE →	e14 prophage, uncharacterized protein pseudogene, portal protein family, e14 prophage,Phage or Prophage Related

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,205,731	0	G	A	100.0%	103.1 / NA	33	*61* (TAG→TAA)  pseudogene (1/412 nt)	ymfR beeE	e14 prophage, uncharacterized protein pseudogene, portal protein family, e14 prophage,Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/18);  total (15/18)

GCGTATGGTGCCTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCA  >  NC_000913/1205603‑1205849                                                                                                                                 |                                                                                                                       gCGTATGGTGCCTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTc                                                                                                              <  2:1614686/139‑1 (MQ=255)         tGCCTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGattat                                                                                                      <  2:604281/139‑1 (MQ=255)           ccTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTAttt                                                                                                    >  2:1595664/1‑139 (MQ=255)                   aTTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGaaa                                                                                            >  1:833039/1‑139 (MQ=255)                      tATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAg                                                                                         <  1:352694/139‑1 (MQ=255)                            ccGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGc                                                                                   <  2:568579/139‑1 (MQ=255)                                            ttgCCGGGGCCCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCtttt                                                                                                               >  2:487772/1‑95 (MQ=255)                                            ttgCCGGGGCCCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCtttt                                                                                                               <  1:487772/95‑1 (MQ=255)                                              gCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCcagc                                                                 >  1:1638821/1‑139 (MQ=255)                                                  gggCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCagag                                                             >  1:309437/1‑139 (MQ=255)                                                                  cTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCg                                             <  2:1638821/139‑1 (MQ=255)                                                                    ggTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCggg                                           <  2:833039/139‑1 (MQ=255)                                                                        gTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGt                                       <  1:1595664/139‑1 (MQ=255)                                                                             tggtggCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTAt                                  <  2:309437/139‑1 (MQ=255)                                                                                     cgATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCta                          <  1:169946/139‑1 (MQ=255)                                                                                                  gTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAg             >  2:1607546/1‑139 (MQ=255)                                                                                                   tACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGAc                                                                          >  2:1404976/1‑77 (MQ=255)                                                                                                   tACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGAc                                                                          <  1:1404976/77‑1 (MQ=255)                                                                                                          tcgtcTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTg                                                                                       <  2:1105923/57‑1 (MQ=255)                                                                                                          tcgtcTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTg                                                                                       >  1:1105923/1‑57 (MQ=255)                                                                                                          tcgtcTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGAc                                                                          >  1:356881/1‑70 (MQ=255)                                                                                                          tcgtcTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGAc                                                                          <  2:356881/70‑1 (MQ=255)                                                                                                          tcgtcTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCggg                                           <  1:22903/101‑1 (MQ=255)                                                                                                          tcgtcTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCggg                                           >  2:22903/1‑101 (MQ=255)                                                                                                          tcgtcTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTAt                                  >  2:949327/1‑110 (MQ=255)                                                                                                          tcgtcTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTAt                                  <  1:949327/110‑1 (MQ=255)                                                                                                             tcTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCg                                             >  1:847665/1‑96 (MQ=255)                                                                                                             tcTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCg                                             <  2:847665/96‑1 (MQ=255)                                                                                                             tcTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCa  >  1:1351225/1‑139 (MQ=255)                                                                                                                      ggAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGc                                                        >  2:1586600/1‑76 (MQ=255)                                                                                                                      ggAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGc                                                        <  1:1586600/76‑1 (MQ=255)                                                                                                                          gTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTAt                                  <  2:284624/94‑1 (MQ=255)                                                                                                                          gTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTAt                                  >  1:284624/1‑94 (MQ=255)                                                                                                                                 |                                                                                                                       GCGTATGGTGCCTGGCTGATTTATCCCCCGGCGGGTTTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCA  >  NC_000913/1205603‑1205849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑