Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,228,724	A→G	T216A (ACT→GCT)	ycgM →	putative isomerase/hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,228,724	0	A	G	100.0%	158.5 / NA	46	T216A (ACT→GCT)	ycgM	putative isomerase/hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/24);  total (22/24)

TCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTT  >  NC_000913/1228588‑1228846                                                                                                                                         |                                                                                                                           tCCGCTGATCGCTTATATGAGCAAGTTTGTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCt                                                                                                                          <  2:413391/139‑1 (MQ=255)  ccGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTc                                                                                                                         >  2:400054/1‑139 (MQ=255)        aTCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGttt                                                                                                                   >  1:280696/1‑139 (MQ=255)                    aGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACtttt                                                                                                       >  1:888153/1‑139 (MQ=255)                      cAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACCGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTg                                                                                                     >  1:824782/1‑139 (MQ=255)                      cAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTg                                                                                                     <  2:966314/139‑1 (MQ=255)                      cAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTg                                                                                                     <  2:888153/139‑1 (MQ=255)                                              tGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACt                                                                             >  2:78648/1‑139 (MQ=255)                                                    tgtgTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATc                                                                       >  2:731918/1‑139 (MQ=255)                                                     gtgtTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTTACAGCTCGCGtt                                                                                                                    >  1:28344/1‑93 (MQ=255)                                                     gtgtTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGtt                                                                                                                    <  2:28344/93‑1 (MQ=255)                                                     gtgtTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCg                                                                      <  2:824782/139‑1 (MQ=255)                                                     gtgtTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCg                                                                      <  2:280696/139‑1 (MQ=255)                                                          gACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCtgtg                                                                 >  2:1379727/1‑139 (MQ=255)                                                                cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                           >  2:28642/1‑139 (MQ=255)                                                                cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                           >  1:421086/1‑139 (MQ=255)                                                                cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                           >  2:1458900/1‑139 (MQ=255)                                                                cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                           <  1:845973/139‑1 (MQ=255)                                                                cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                           <  1:1379727/139‑1 (MQ=255)                                                                   gCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTgg                                                        <  1:1458900/139‑1 (MQ=255)                                                                        aTGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGc                                                                >  2:1447061/1‑126 (MQ=255)                                                                        aTGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGc                                                                <  1:1447061/126‑1 (MQ=255)                                                                              tCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCGTCGCTGTGCCAGACTGGTTATAAGGTGc                                             >  1:452146/1‑139 (MQ=255)                                                                               cGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTcgcg                                                                                                                      <  1:895641/65‑1 (MQ=255)                                                                               cGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTcgcg                                                                                                                      >  2:895641/1‑65 (MQ=255)                                                                                 gCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGtt                                          >  2:168172/1‑139 (MQ=255)                                                                                      ttGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAAc                                     >  1:1636828/1‑139 (MQ=255)                                                                                       tGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTgg                                                        >  1:787332/1‑119 (MQ=255)                                                                                       tGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTgg                                                        <  2:787332/119‑1 (MQ=255)                                                                                          aaaGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTaa                                 <  2:421086/139‑1 (MQ=255)                                                                                           aaGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAAt                                <  1:78648/139‑1 (MQ=255)                                                                                                 tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTcgcg                                                                                                                      >  1:74620/1‑47 (MQ=255)                                                                                                 tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTcgcg                                                                                                                      <  2:74620/47‑1 (MQ=255)                                                                                                 tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTg                                                                                                     <  2:415779/64‑1 (MQ=255)                                                                                                 tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTg                                                                                                     >  1:415779/1‑64 (MQ=255)                                                                                                  gatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGaa                         <  1:168172/139‑1 (MQ=255)                                                                                                   atgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGtt                                                                                                                    <  2:985212/47‑1 (MQ=255)                                                                                                   atgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGtt                                                                                                                    >  1:985212/1‑47 (MQ=255)                                                                                                     gaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAacac                      <  1:400054/139‑1 (MQ=255)                                                                                                           aCAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGc                                                                          >  2:769669/1‑81 (MQ=255)                                                                                                           aCAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGc                                                                          <  1:769669/81‑1 (MQ=255)                                                                                                           aCAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCtgatg                >  2:922161/1‑139 (MQ=255)                                                                                                               tCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCg            <  1:28642/139‑1 (MQ=255)                                                                                                               tCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAAGGGCGGAACACCTGATGAGCg            <  2:418337/139‑1 (MQ=255)                                                                                                                   tttCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCGTCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGt        <  2:452146/139‑1 (MQ=255)                                                                                                                         tGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCttt  <  1:621562/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                           TCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTT  >  NC_000913/1228588‑1228846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑