Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,260,528	A→G	I92I (ATT→ATC)	dauA ←	C4‑dicarboxylic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,260,528	0	A	G	100.0%	229.5 / NA	63	I92I (ATT→ATC)	dauA	C4‑dicarboxylic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/29);  total (34/29)

GGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGAATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAA  >  NC_000913/1260402‑1260649                                                                                                                               |                                                                                                                          ggAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAAt                                                                                                               >  2:1318156/1‑139 (MQ=255)                 tCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGt                                                                                                       <  1:1096280/131‑1 (MQ=255)                 tCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGt                                                                                                       >  2:1096280/1‑131 (MQ=255)                 tCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGAc                                                                                                  >  2:740889/1‑136 (MQ=255)                 tCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGAc                                                                                                  <  1:740889/136‑1 (MQ=255)                    aTCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGaaa                                                                                            >  2:1376053/1‑139 (MQ=255)                        ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGa                                                                                                   <  2:1580443/128‑1 (MQ=255)                        ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGa                                                                                                   >  1:1580443/1‑128 (MQ=255)                        ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCt                                                                                        >  2:322616/1‑139 (MQ=255)                        ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCt                                                                                        >  2:1138134/1‑139 (MQ=255)                         gCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTa                                                                                       >  2:735624/1‑139 (MQ=255)                         gCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTa                                                                                       >  1:264899/1‑139 (MQ=255)                           gACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGa                                                                                                   >  2:873276/1‑125 (MQ=255)                           gACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGa                                                                                                   <  1:873276/125‑1 (MQ=255)                                 aGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGt                                                                                                       >  2:519630/1‑115 (MQ=255)                                 aGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGt                                                                                                       <  1:519630/115‑1 (MQ=255)                                        cAGACCCATCAGAATGAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGt                                                                                                       <  1:1380149/108‑1 (MQ=255)                                        cAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGt                                                                                                       >  2:408925/1‑108 (MQ=255)                                        cAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGt                                                                                                       <  1:408925/108‑1 (MQ=255)                                        cAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGt                                                                                                       >  2:1380149/1‑108 (MQ=255)                                           aCCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTTCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGaccca                                                                           <  2:1332717/133‑1 (MQ=255)                                           aCCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGACCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGaccca                                                                           >  1:1332717/1‑133 (MQ=255)                                               aTCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTGGCGACACGGGATAGAGGATTACCACAAATGCGGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                 >  1:1086999/1‑139 (MQ=255)                                                tCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCACAGc                                                                >  2:1083558/1‑139 (MQ=255)                                                     aTCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGa                                                           <  1:294563/139‑1 (MQ=255)                                                      tCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGAc                                                          >  2:964870/1‑139 (MQ=255)                                                       cAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACa                                                         <  1:1376053/139‑1 (MQ=255)                                                         aaaaGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTa                                                                                       <  1:632102/107‑1 (MQ=255)                                                         aaaaGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTa                                                                                       >  2:632102/1‑107 (MQ=255)                                                              aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCg                                                  >  1:365815/1‑139 (MQ=255)                                                                     aCAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGc                                           >  1:982511/1‑139 (MQ=255)                                                                        gCAAGGTCGCAACCCGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACGAATGCCGCAGTCGGAACGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                                        >  1:1185289/1‑139 (MQ=255)                                                                        gCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                                        <  1:1138134/139‑1 (MQ=255)                                                                        gCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                                        <  1:1318156/139‑1 (MQ=255)                                                                         cAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                 <  1:1186712/113‑1 (MQ=255)                                                                         cAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                 >  2:1186712/1‑113 (MQ=255)                                                                         cAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCg                                       <  2:365815/139‑1 (MQ=255)                                                                            ggTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCt                                                                                        <  1:722689/87‑1 (MQ=255)                                                                            ggTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCt                                                                                        >  2:722689/1‑87 (MQ=255)                                                                              tCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGtata                                  >  2:852093/1‑139 (MQ=255)                                                                                  aaCCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATaaa                              <  1:1083558/139‑1 (MQ=255)                                                                                        cagTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTAc                        >  1:593766/1‑139 (MQ=255)                                                                                          gTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGt                          >  1:155036/1‑135 (MQ=255)                                                                                          gTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGt                          <  2:155036/135‑1 (MQ=255)                                                                                           tCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTgg                     <  1:1377575/139‑1 (MQ=255)                                                                                           tCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTgg                     <  2:264899/139‑1 (MQ=255)                                                                                             cTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTgggg                   <  1:964870/139‑1 (MQ=255)                                                                                             cTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTgggg                   <  1:735624/139‑1 (MQ=255)                                                                                                    tCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcaca            >  1:331753/1‑139 (MQ=255)                                                                                                     ccAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTg                                                                                <  2:573026/70‑1 (MQ=255)                                                                                                     ccAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTg                                                                                >  1:811225/1‑70 (MQ=255)                                                                                                     ccAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTg                                                                                >  1:573026/1‑70 (MQ=255)                                                                                                     ccAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTg                                                                                <  2:811225/70‑1 (MQ=255)                                                                                                     ccAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCATCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTg                      <  2:1315797/128‑1 (MQ=255)                                                                                                     ccAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCATCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTg                      >  1:1315797/1‑128 (MQ=255)                                                                                                     ccAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcaca            >  1:999143/1‑138 (MQ=255)                                                                                                     ccAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcaca            <  2:999143/138‑1 (MQ=255)                                                                                                     ccAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCacac           >  2:421729/1‑139 (MQ=255)                                                                                                        aaCTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCAcaccac        <  2:593766/139‑1 (MQ=255)                                                                                                        aaCTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCAcaccac        <  1:1427703/139‑1 (MQ=255)                                                                                                              tGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCaa  >  1:490794/1‑139 (MQ=255)                                                                                                                           agagGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCg                                       <  1:821832/89‑1 (MQ=255)                                                                                                                           agagGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCg                                       >  2:821832/1‑89 (MQ=255)                                                                                                                               |                                                                                                                          GGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGAATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAA  >  NC_000913/1260402‑1260649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑