Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,454,292	C→A	G122G (GGC→GGA)	paaA →	ring 1,2‑phenylacetyl‑CoA epoxidase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,454,292	0	C	A	100.0%	159.3 / NA	49	G122G (GGC→GGA)	paaA	ring 1,2‑phenylacetyl‑CoA epoxidase subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/26);  total (23/26)

TATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCA  >  NC_000913/1454158‑1454425                                                                                                                                       |                                                                                                                                      tATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCggatggc                                                                                                                                   >  2:1088665/1‑139 (MQ=255)        ataGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGa                                                                                                                            <  1:934193/139‑1 (MQ=255)                 ccgAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGa                                                                                                                            <  2:1400493/130‑1 (MQ=255)                 ccgAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGa                                                                                                                            >  1:1400493/1‑130 (MQ=255)                 ccgAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGc                                                                                                                   <  1:437485/139‑1 (MQ=255)                 ccgAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGc                                                                                                                   >  2:437485/1‑139 (MQ=255)                    aaaCACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGAt                                                                                                                <  1:1436672/139‑1 (MQ=255)                    aaaCACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGAt                                                                                                                <  1:1031367/139‑1 (MQ=255)                               tGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAgg                                                                                                     <  1:1262064/139‑1 (MQ=255)                                 cgcCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTg                                                                                                   >  2:1556633/1‑139 (MQ=255)                                                cTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAct                                                                                    >  2:905009/1‑139 (MQ=255)                                                cTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAct                                                                                    >  2:906928/1‑139 (MQ=255)                                                      aaaaaTGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAc                                                                                     <  1:646132/132‑1 (MQ=255)                                                      aaaaaTGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAc                                                                                     >  2:646132/1‑132 (MQ=255)                                                       aaaaTGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             <  1:981445/139‑1 (MQ=255)                                                       aaaaTGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             <  1:1556633/139‑1 (MQ=255)                                                               cGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGcc                                                                                         <  1:644695/119‑1 (MQ=255)                                                               cGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGcc                                                                                         >  2:644695/1‑119 (MQ=255)                                                                                  tcctccATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCaa                                                           >  2:830738/1‑130 (MQ=255)                                                                                  tcctccATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCaa                                                           <  1:830738/130‑1 (MQ=255)                                                                                   cctccATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCaa                                                           >  2:714029/1‑129 (MQ=255)                                                                                   cctccATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCaa                                                           <  1:714029/129‑1 (MQ=255)                                                                                    ctccATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCg                                                                                        >  2:184227/1‑99 (MQ=255)                                                                                    ctccATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCg                                                                                        <  1:184227/99‑1 (MQ=255)                                                                                                   tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             >  2:1415108/1‑95 (MQ=255)                                                                                                   tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             <  1:1415108/95‑1 (MQ=255)                                                                                                   tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        <  1:911938/100‑1 (MQ=255)                                                                                                   tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        >  2:911938/1‑100 (MQ=255)                                                                                                      gACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAagaaga                                     >  1:924418/1‑132 (MQ=255)                                                                                                      gACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAagaaga                                     <  2:924418/132‑1 (MQ=255)                                                                                                        cATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCa                            >  1:856473/1‑139 (MQ=255)                                                                                                          ttGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCAcc                          <  1:906928/139‑1 (MQ=255)                                                                                                             aGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAg                                                                                                                    >  1:628319/1‑46 (MQ=255)                                                                                                             aGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAg                                                                                                                    <  2:628319/46‑1 (MQ=255)                                                                                                             aGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTa                                                                      >  1:921886/1‑92 (MQ=255)                                                                                                             aGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTa                                                                      <  2:921886/92‑1 (MQ=255)                                                                                                             aGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGc                       >  1:1278317/1‑139 (MQ=255)                                                                                                                 gggCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGAt                                                                                                                >  1:696465/1‑46 (MQ=255)                                                                                                                 gggCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGAt                                                                                                                <  2:696465/46‑1 (MQ=255)                                                                                                                  ggCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             <  1:920999/80‑1 (MQ=255)                                                                                                                  ggCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             >  2:920999/1‑80 (MQ=255)                                                                                                                       atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        <  1:1496552/80‑1 (MQ=255)                                                                                                                       atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        >  2:1496552/1‑80 (MQ=255)                                                                                                                       atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGc                                                             >  1:906606/1‑91 (MQ=255)                                                                                                                       atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGc                                                             <  2:906606/91‑1 (MQ=255)                                                                                                                       atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGaaa                                                   >  2:1045917/1‑101 (MQ=255)                                                                                                                       atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGaaa                                                   <  1:1045917/101‑1 (MQ=255)                                                                                                                            ggTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAc                                                                                     <  1:73999/62‑1 (MQ=255)                                                                                                                            ggTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAc                                                                                     >  2:73999/1‑62 (MQ=255)                                                                                                                                  aTCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCa  <  1:1088665/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      TATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCA  >  NC_000913/1454158‑1454425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑