Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,493,219 A→G E250E (GAA→GAG)  trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,493,2190AG100.0% 229.0 / NA 64E250E (GAA→GAG) trgmethyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/32);  total (32/32)

GGCGCGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAACCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAG  >  NC_000913/1493085‑1493349
                                                                                                                                      |                                                                                                                                  
ggCGCGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcgg                                                                                                                                <  2:925639/139‑1 (MQ=255)
  cgcgTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGGCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggcg                                                                                                                              >  1:272078/1‑139 (MQ=255)
     gTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt                                                                                                                           <  2:1130019/139‑1 (MQ=255)
          tgcctgccCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTaa                                                                                                                      >  1:1073912/1‑139 (MQ=255)
               gCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGaaa                                                                                                                 >  2:323370/1‑139 (MQ=255)
                  cTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGACCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCg                                                                                                              <  1:1142776/139‑1 (MQ=255)
                    ggTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                               >  1:678157/1‑120 (MQ=255)
                    ggTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                               <  2:678157/120‑1 (MQ=255)
                      tCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCg                                                                                                          >  2:878077/1‑139 (MQ=255)
                          gACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt                                                                                                                           <  1:1248057/118‑1 (MQ=255)
                          gACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt                                                                                                                           >  2:1248057/1‑118 (MQ=255)
                          gACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTa                                                                                                      <  1:878077/139‑1 (MQ=255)
                           aCGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTaa                                                                                                     >  1:84354/1‑139 (MQ=255)
                            cGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCACAGATCGGATTGAAAAAATCGCCAGGGGCGATCTGACGATGGATGATGGGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAg                                                                                                    <  1:1539948/139‑1 (MQ=255)
                                       tttATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAgtcg                                                                                                 <  1:1223127/131‑1 (MQ=255)
                                       tttATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAgtcg                                                                                                 >  2:1223127/1‑131 (MQ=255)
                                              tGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt                                                                                  <  1:57331/139‑1 (MQ=255)
                                              tGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt                                                                                  <  2:272078/139‑1 (MQ=255)
                                                cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAATATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCtt                                                                                                       <  1:1093456/116‑1 (MQ=255)
                                                cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCtt                                                                                                       >  2:1093456/1‑116 (MQ=255)
                                                cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgc                                                                                >  1:156189/1‑139 (MQ=255)
                                                   cGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCCGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                               >  2:1593488/1‑89 (MQ=255)
                                                   cGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                               <  1:1593488/89‑1 (MQ=255)
                                                        gATCGTCATTCGTCCACTGCAATATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGt                                                                                                            <  2:826979/103‑1 (MQ=255)
                                                        gATCGTCATTCGTCCACTGCAATATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGt                                                                                                            >  1:826979/1‑103 (MQ=255)
                                                        gATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCg                                                                                                              >  2:840818/1‑101 (MQ=255)
                                                        gATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCg                                                                                                              <  1:840818/101‑1 (MQ=255)
                                                                ttCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGAt                                                                <  2:84354/139‑1 (MQ=255)
                                                                   gTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGAc                                                             >  1:1477888/1‑139 (MQ=255)
                                                                       aCTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTa                                                         >  1:455521/1‑139 (MQ=255)
                                                                         tGCAACATGCCGGACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGGGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGGCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAgg                                                       <  1:1409399/139‑1 (MQ=255)
                                                                         tGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCAtt                                                                          >  2:952400/1‑120 (MQ=255)
                                                                         tGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCAtt                                                                          <  1:952400/120‑1 (MQ=255)
                                                                                tGCCGCACAACGGATTGAAAAAATCGCGAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTAACTGGGGATGACAGTAGGGACGGtt                                                >  2:1079379/1‑139 (MQ=255)
                                                                                   cGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACt                                                                      >  1:1590253/1‑114 (MQ=255)
                                                                                   cGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACt                                                                      <  2:1590253/114‑1 (MQ=255)
                                                                                     cacaACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAg                                                                                                    >  2:1570772/1‑82 (MQ=255)
                                                                                     cacaACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAg                                                                                                    <  1:1570772/82‑1 (MQ=255)
                                                                                     cacaACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGa                                                                 >  1:113951/1‑117 (MQ=255)
                                                                                     cacaACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGa                                                                 <  2:113951/117‑1 (MQ=255)
                                                                                         aCGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATg                                                               >  2:767297/1‑115 (MQ=255)
                                                                                         aCGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATg                                                               <  1:767297/115‑1 (MQ=255)
                                                                                         aCGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGa                                                     >  2:36756/1‑125 (MQ=255)
                                                                                         aCGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGa                                                     <  1:36756/125‑1 (MQ=255)
                                                                                             aTTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                               >  1:1210241/1‑47 (MQ=255)
                                                                                             aTTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc                                                                                                                               <  2:1210241/47‑1 (MQ=255)
                                                                                             aTTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCgg                                   <  2:455521/139‑1 (MQ=255)
                                                                                              ttGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGa                                  <  1:323370/139‑1 (MQ=255)
                                                                                                    aaaTCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGAt                            >  1:485890/1‑139 (MQ=255)
                                                                                                              tGGCGATCTGACGATGAATGATGAGCCGGCGGGACGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGc                  >  2:1378911/1‑139 (MQ=255)
                                                                                                                          gATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAgtcgt                                                                                                <  2:364265/49‑1 (MQ=255)
                                                                                                                          gATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAgtcgt                                                                                                >  1:364265/1‑49 (MQ=255)
                                                                                                                              aatgaTGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATg                                                               <  2:359201/78‑1 (MQ=255)
                                                                                                                              aatgaTGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATg                                                               >  1:359201/1‑78 (MQ=255)
                                                                                                                              aatgaTGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAg  <  2:156189/139‑1 (MQ=255)
                                                                                                                              aatgaTGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTAATCGTGGCACCAGCGAAATTTCAg  >  2:97775/1‑139 (MQ=255)
                                                                                                                               atgatgAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATg                                                               <  2:951634/77‑1 (MQ=255)
                                                                                                                               atgatgAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATg                                                               >  1:951634/1‑77 (MQ=255)
                                                                                                                                tgatgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgcagc                                                                             <  2:375568/62‑1 (MQ=255)
                                                                                                                                tgatgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgcagc                                                                             >  1:375568/1‑62 (MQ=255)
                                                                                                                                  atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCaaa                                                                                   <  2:883855/54‑1 (MQ=255)
                                                                                                                                  atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCaaa                                                                                   >  1:883855/1‑54 (MQ=255)
                                                                                                                                  atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgcagca                                                                            >  2:418195/1‑61 (MQ=255)
                                                                                                                                  atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgcagca                                                                            <  1:418195/61‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
GGCGCGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAACCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAG  >  NC_000913/1493085‑1493349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑