Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,513,468	T→C	S218P (TCC→CCC)	ydcT →	putative ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,513,468	0	T	C	100.0%	200.2 / NA	55	S218P (TCC→CCC)	ydcT	putative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/26);  total (29/26)

AGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATTCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACA  >  NC_000913/1513337‑1513601                                                                                                                                    |                                                                                                                                      aGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                                <  1:19590/139‑1 (MQ=255)   cTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                              <  1:1275454/139‑1 (MQ=255)     ggAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAt                                                                                                                            <  2:1609782/139‑1 (MQ=255)       aaCTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAtct                                                                                                                          >  1:242758/1‑139 (MQ=255)             aaaaaCTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                                >  2:1626982/1‑127 (MQ=255)             aaaaaCTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                                <  1:1626982/127‑1 (MQ=255)                           tctctcGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCTGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGc                                                                                                      >  2:1070991/1‑139 (MQ=255)                                     tCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGc                                                                                            >  2:1434436/1‑139 (MQ=255)                                     tCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGc                                                                                            >  2:1217734/1‑139 (MQ=255)                                           ttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                              <  2:1571210/99‑1 (MQ=255)                                           ttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                              >  1:1571210/1‑99 (MQ=255)                                           ttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                      >  2:77371/1‑139 (MQ=255)                                             aTCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCg                                                                                    >  2:1466179/1‑139 (MQ=255)                                             aTCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCg                                                                                    <  1:1466179/139‑1 (MQ=255)                                                   gTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTATGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTa                                                                              >  2:1091002/1‑139 (MQ=255)                                                         cACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGa                                                                        <  1:77371/139‑1 (MQ=255)                                                            gATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAt                                                                                                                            >  1:1588771/1‑84 (MQ=255)                                                            gATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAt                                                                                                                            <  2:1588771/84‑1 (MQ=255)                                                            gATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGaa                                                                       >  2:1389670/1‑137 (MQ=255)                                                            gATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGaa                                                                       <  1:1389670/137‑1 (MQ=255)                                                                    cGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGAt                                                             <  1:1091002/139‑1 (MQ=255)                                                                    cGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGAt                                                             <  1:1217734/139‑1 (MQ=255)                                                                    cGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGAt                                                             <  2:242758/139‑1 (MQ=255)                                                                     gAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCg                                                                                          <  1:114704/109‑1 (MQ=255)                                                                     gAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCg                                                                                          >  2:114704/1‑109 (MQ=255)                                                                      aaGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                      >  1:511338/1‑112 (MQ=255)                                                                      aaGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                      <  2:511338/112‑1 (MQ=255)                                                                      aaGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGAt                                                             >  2:285945/1‑137 (MQ=255)                                                                      aaGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGAt                                                             <  1:285945/137‑1 (MQ=255)                                                                       aGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                              >  1:1158552/1‑71 (MQ=255)                                                                       aGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                              <  2:1158552/71‑1 (MQ=255)                                                                       aGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGa                                                          <  2:1617865/139‑1 (MQ=255)                                                                                     ccGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACTCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGaa                                            <  1:1434436/139‑1 (MQ=255)                                                                                        aTCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCggg                                                                                        <  2:605227/92‑1 (MQ=255)                                                                                        aTCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCggg                                                                                        >  1:605227/1‑92 (MQ=255)                                                                                              tgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTgg                                   >  1:1496307/1‑139 (MQ=255)                                                                                                gCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGAt                                                             <  1:256386/111‑1 (MQ=255)                                                                                                gCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGAt                                                             >  2:256386/1‑111 (MQ=255)                                                                                                   gTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGAt                                                             >  1:707786/1‑108 (MQ=255)                                                                                                   gTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGAt                                                             <  2:707786/108‑1 (MQ=255)                                                                                                           taatGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                      <  2:1634882/75‑1 (MQ=255)                                                                                                           taatGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                      >  1:1634882/1‑75 (MQ=255)                                                                                                              tGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTc                   >  1:382623/1‑139 (MQ=255)                                                                                                                gACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCa                                                                                                         >  1:590840/1‑51 (MQ=255)                                                                                                                gACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCa                                                                                                         <  2:590840/51‑1 (MQ=255)                                                                                                                gACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGc                 >  2:1245565/1‑139 (MQ=255)                                                                                                                  cGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGccc               >  1:70879/1‑139 (MQ=255)                                                                                                                     aTTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCgcgc                                                                                                          <  2:223842/45‑1 (MQ=255)                                                                                                                     aTTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCgcgc                                                                                                          >  1:223842/1‑45 (MQ=255)                                                                                                                     aTTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGc            >  1:199977/1‑139 (MQ=255)                                                                                                                        gAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGAc         <  2:70879/139‑1 (MQ=255)                                                                                                                         aGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGAcc        >  2:266019/1‑139 (MQ=255)                                                                                                                               tCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGa                                                                        <  2:500492/69‑1 (MQ=255)                                                                                                                               tCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGa                                                                        >  1:500492/1‑69 (MQ=255)                                                                                                                               tCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGAAGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACa  >  1:1012213/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                      AGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATTCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACA  >  NC_000913/1513337‑1513601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑