Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,598,617	C→T	*531* (TAG→TAA)	lsrK ←	autoinducer‑2 (AI‑2) kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,598,617	0	C	T	100.0%	223.4 / NA	69	*531* (TAG→TAA)	lsrK	autoinducer‑2 (AI‑2) kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/33);  total (36/33)

CGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTG  >  NC_000913/1598489‑1598744                                                                                                                                 |                                                                                                                                cGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAg                                                                                                                       >  1:287778/1‑139 (MQ=255)    aCTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGgcg                                                                                                                    <  2:694924/139‑1 (MQ=255)      tGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCt                                                                                                                  >  2:22883/1‑139 (MQ=255)           aTGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGgcg                                                                                                                    >  2:1450112/1‑132 (MQ=255)           aTGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGgcg                                                                                                                    <  1:1450112/132‑1 (MQ=255)                  gATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAacgac                                                                                                      <  1:22883/139‑1 (MQ=255)                            cAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTc                                                                                            >  2:887711/1‑139 (MQ=255)                                 tGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGa                                                                                       >  1:1408409/1‑139 (MQ=255)                                 tGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGa                                                                                       <  1:887711/139‑1 (MQ=255)                                  ggCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGAt                                                                                      >  1:788832/1‑139 (MQ=255)                                       ttAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAAcc                                                                                 <  2:1638216/139‑1 (MQ=255)                                       ttAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAAcc                                                                                 <  2:287778/139‑1 (MQ=255)                                             aGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGcccc                                                                           >  2:448139/1‑139 (MQ=255)                                             aGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGcccc                                                                           >  1:967102/1‑139 (MQ=255)                                              gCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                 <  2:1385902/116‑1 (MQ=255)                                              gCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                 >  1:1385902/1‑116 (MQ=255)                                                cAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGc                                                                        <  1:448139/139‑1 (MQ=255)                                                   ctctAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGa                                                                     <  2:1408409/139‑1 (MQ=255)                                                             agGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAg                                                                         >  1:153745/1‑125 (MQ=255)                                                             agGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAg                                                                         <  2:153745/125‑1 (MQ=255)                                                             agGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATaaa                                                           >  2:1024164/1‑139 (MQ=255)                                                              gggAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGgcg                                                                                                                    >  2:628181/1‑81 (MQ=255)                                                              gggAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGgcg                                                                                                                    <  1:628181/81‑1 (MQ=255)                                                                     aaGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgcctgc                                                   <  1:1024164/139‑1 (MQ=255)                                                                      aGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAg                                                                         >  1:642947/1‑116 (MQ=255)                                                                      aGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAg                                                                         <  2:642947/116‑1 (MQ=255)                                                                             cTATACTCAAAAACTAACAGCCACAGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATc                                           >  1:1477649/1‑139 (MQ=255)                                                                                taCTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATcgcg                                        >  2:89096/1‑139 (MQ=255)                                                                                  cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTg                                      >  2:258252/1‑139 (MQ=255)                                                                                    cAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGaa                                    <  1:258252/139‑1 (MQ=255)                                                                                     aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgc                                                       >  1:1630567/1‑119 (MQ=255)                                                                                     aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgc                                                       <  2:1630567/119‑1 (MQ=255)                                                                                     aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTg                                      <  1:602496/136‑1 (MQ=255)                                                                                     aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTg                                      >  2:602496/1‑136 (MQ=255)                                                                                       aaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAAccc                                                                                                                         <  2:563305/51‑1 (MQ=255)                                                                                       aaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAAccc                                                                                                                         >  1:563305/1‑51 (MQ=255)                                                                                       aaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATcc                                 >  2:537227/1‑139 (MQ=255)                                                                                                     ggTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAg                                                                         >  1:478495/1‑85 (MQ=255)                                                                                                     ggTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAg                                                                         <  2:478495/85‑1 (MQ=255)                                                                                                         atcatGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                 >  1:887351/1‑57 (MQ=255)                                                                                                         atcatGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                 <  2:887351/57‑1 (MQ=255)                                                                                                           catGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTc                                                                                            <  1:1479089/60‑1 (MQ=255)                                                                                                           catGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTc                                                                                            >  2:1479089/1‑60 (MQ=255)                                                                                                           catGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc             <  1:89096/139‑1 (MQ=255)                                                                                                           catGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc             <  2:1477649/139‑1 (MQ=255)                                                                                                             tgatgTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCg           <  2:788832/139‑1 (MQ=255)                                                                                                                tgtgGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGt        >  1:1370270/1‑139 (MQ=255)                                                                                                                 gtgGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATg                   <  1:1641468/127‑1 (MQ=255)                                                                                                                 gtgGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATg                   >  2:1641468/1‑127 (MQ=255)                                                                                                                   ggCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGAt                             >  2:546918/1‑115 (MQ=255)                                                                                                                   ggCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGAt                             <  1:546918/115‑1 (MQ=255)                                                                                                                    gCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAacga                                                                                                       >  1:1370811/1‑40 (MQ=255)                                                                                                                    gCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAacga                                                                                                       <  2:1370811/40‑1 (MQ=39)                                                                                                                    gCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAAcc                                                                                 >  1:1018049/1‑62 (MQ=255)                                                                                                                    gCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAAcc                                                                                 <  2:1018049/62‑1 (MQ=255)                                                                                                                    gCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATc                                                                   <  1:679018/76‑1 (MQ=255)                                                                                                                    gCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATc                                                                   >  2:679018/1‑76 (MQ=255)                                                                                                                     cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAacgacg                                                                                                     >  1:1410854/1‑41 (MQ=255)                                                                                                                     cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAacgacg                                                                                                     <  2:1410854/41‑1 (MQ=255)                                                                                                                     cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATg                                                                                        >  1:99738/1‑54 (MQ=255)                                                                                                                     cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATg                                                                                        <  2:99738/54‑1 (MQ=255)                                                                                                                     cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGa                                                                     >  1:1250443/1‑73 (MQ=255)                                                                                                                     cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGa                                                                     <  2:1250443/73‑1 (MQ=255)                                                                                                                     cTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgt   >  2:211776/1‑139 (MQ=255)                                                                                                                      tGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgtg  >  2:1338684/1‑139 (MQ=255)                                                                                                                        tCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                 <  1:1050161/42‑1 (MQ=255)                                                                                                                        tCAATGAAATTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                 >  2:1050161/1‑42 (MQ=255)                                                                                                                         cAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCC‑GCTGATCCTGATAAACTGCCTGCCACTTATcgcg                                        >  1:1170231/1‑97 (MQ=255)                                                                                                                         cAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCC‑GCTGATCCTGATAAACTGCCTGCCACTTATcgcg                                        <  2:1170231/97‑1 (MQ=255)                                                                                                                                 |                                                                                                                                CGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTG  >  NC_000913/1598489‑1598744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑