Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,606,561	A→G	K154K (AAA→AAG)	lsrF →	putative autoinducer‑2 (AI‑2) aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,606,561	0	A	G	100.0%	128.4 / NA	36	K154K (AAA→AAG)	lsrF	putative autoinducer‑2 (AI‑2) aldolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/16);  total (20/16)

CGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCC  >  NC_000913/1606446‑1606681                                                                                                                    |                                                                                                                         cGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGc                                                                                                   >  2:429439/1‑139 (MQ=255) cGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGc                                                                                                   >  1:362742/1‑139 (MQ=255)               gAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTgg                                                                                     <  1:389240/139‑1 (MQ=255)                    cGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAAtatta                                                                                                                   >  2:872242/1‑104 (MQ=255)                    cGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAAtatta                                                                                                                   <  1:872242/104‑1 (MQ=255)                                        aCGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGaa                                                                                              >  2:473745/1‑105 (MQ=255)                                        aCGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGaa                                                                                              <  1:473745/105‑1 (MQ=255)                                               gcgcCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGc                                                     >  2:1209724/1‑139 (MQ=255)                                                           ttGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTggg                                                                                    >  1:1571092/1‑96 (MQ=255)                                                           ttGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTggg                                                                                    <  2:1571092/96‑1 (MQ=255)                                                            tGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAg                                                                                                              <  1:1578890/69‑1 (MQ=255)                                                            tGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAg                                                                                                              >  2:1578890/1‑69 (MQ=255)                                                             gcgcGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTc                                                                                                                >  1:1004912/1‑66 (MQ=255)                                                             gcgcGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTc                                                                                                                <  2:1004912/66‑1 (MQ=255)                                                                     gcggcgCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGc                               >  2:546976/1‑139 (MQ=255)                                                                     gcggcgCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGc                               >  2:1207145/1‑139 (MQ=255)                                                                                        gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTgg                                                                                     >  2:1605730/1‑66 (MQ=255)                                                                                        gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTgg                                                                                     <  1:1605730/66‑1 (MQ=255)                                                                                        gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTg                                         <  1:102354/110‑1 (MQ=255)                                                                                        gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTg                                         >  2:102354/1‑110 (MQ=255)                                                                                        gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGAc            >  2:527512/1‑139 (MQ=255)                                                                                        gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGAc            >  1:1611536/1‑139 (MQ=255)                                                                                              aaTATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAAt      <  1:1209724/139‑1 (MQ=255)                                                                                              aaTATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAAt      <  1:531740/139‑1 (MQ=255)                                                                                              aaTATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAAt      >  2:531740/1‑139 (MQ=255)                                                                                                  tGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATcgcc  <  1:546976/139‑1 (MQ=255)                                                                                                      cATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCa                                                    <  2:204541/85‑1 (MQ=255)                                                                                                      cATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCa                                                    >  1:204541/1‑85 (MQ=255)                                                                                                        tCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTg                                                        <  1:953488/79‑1 (MQ=255)                                                                                                        tCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTg                                                        >  2:953488/1‑79 (MQ=255)                                                                                                           gTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGa                                                                >  1:1458152/1‑68 (MQ=255)                                                                                                           gTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGa                                                                <  2:1458152/68‑1 (MQ=255)                                                                                                           gTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATcgcc  <  2:264571/130‑1 (MQ=255)                                                                                                           gTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATcgcc  >  1:264571/1‑130 (MQ=255)                                                                                                              gATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGc                                                                     <  2:637689/60‑1 (MQ=255)                                                                                                              gATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGc                                                                     >  1:637689/1‑60 (MQ=255)                                                                                                                    |                                                                                                                         CGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCC  >  NC_000913/1606446‑1606681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑