Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,630,986 A→G intergenic (‑73/+16) ydfI ← / ← ydfJ putative NAD‑dependent D‑mannonate oxidoreductase/pseudogene, MFS transporter family, interrupted by Qin prophage,Phage or Prophage Related, putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,630,9860AG100.0% 224.9 / NA 63intergenic (‑73/+16)ydfI/ydfJputative NAD‑dependent D‑mannonate oxidoreductase/pseudogene, MFS transporter family, interrupted by Qin prophage,Phage or Prophage Related, putative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/29);  total (34/29)

CATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGAGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCACGGTCTTTCACCTCTGGCATTTTCAGCGCA  >  NC_000913/1630868‑1631110
                                                                                                                      |                                                                                                                            
cATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACa                                                                                                          <  2:17789/139‑1 (MQ=255)
 aTAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAg                                                                                                         >  1:941886/1‑139 (MQ=255)
   aaaCAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTa                                                                                                                    >  1:205439/1‑126 (MQ=255)
   aaaCAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTa                                                                                                                    <  2:205439/126‑1 (MQ=255)
   aaaCAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGAc                                                                                                       <  2:925569/139‑1 (MQ=255)
   aaaCAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGAc                                                                                                       <  2:941886/139‑1 (MQ=255)
        gggAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTg                                                                                                  >  1:857082/1‑139 (MQ=255)
             tgtCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAgg                                                                                             >  1:463315/1‑139 (MQ=255)
               tCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACa                                                                                                          >  1:1262550/1‑124 (MQ=255)
               tCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACa                                                                                                          <  2:1262550/124‑1 (MQ=255)
                                       ttCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAg                                                                                     >  1:1374958/1‑121 (MQ=255)
                                       ttCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAg                                                                                     <  2:1374958/121‑1 (MQ=255)
                                                cAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACa                                                                                                          <  2:390328/91‑1 (MQ=255)
                                                cAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACa                                                                                                          >  1:390328/1‑91 (MQ=255)
                                                   ttCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAacac                                                                         <  1:1534070/121‑1 (MQ=255)
                                                   ttCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAacac                                                                         >  2:1534070/1‑121 (MQ=255)
                                                   ttCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAg                                                       >  1:1549822/1‑139 (MQ=255)
                                                               aaatatGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAgcg                                           <  2:1506637/139‑1 (MQ=255)
                                                                aatatGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGaa                                                                                                                           <  2:430747/58‑1 (MQ=255)
                                                                aatatGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGaa                                                                                                                           >  1:430747/1‑58 (MQ=255)
                                                                aatatGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAgcgc                                          >  2:1585765/1‑139 (MQ=255)
                                                                    tGGCAAGCTATATGTTTTGTTATATGAATCAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTacaaca                                                                          >  1:963176/1‑103 (MQ=255)
                                                                    tGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACa                                                                                                          >  2:117824/1‑71 (MQ=255)
                                                                    tGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACa                                                                                                          <  1:117824/71‑1 (MQ=255)
                                                                    tGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTacaaca                                                                          <  2:963176/103‑1 (MQ=255)
                                                                     ggCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGaa                                                                                                                           <  1:396793/53‑1 (MQ=255)
                                                                     ggCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGaa                                                                                                                           >  2:396793/1‑53 (MQ=255)
                                                                     ggCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTa                                     >  1:343136/1‑139 (MQ=255)
                                                                      gCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTaa                                    >  1:1083067/1‑139 (MQ=255)
                                                                       cAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGaa                                                                                                                           >  2:96518/1‑51 (MQ=255)
                                                                       cAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGaa                                                                                                                           <  1:96518/51‑1 (MQ=255)
                                                                          gCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCa                                <  1:1585765/139‑1 (MQ=255)
                                                                              tatGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTACGGGTTTACAGACTTCTGGAAGGTTgc                                                                                         >  2:1205274/1‑78 (MQ=255)
                                                                              tatGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTgc                                                                                         <  1:1205274/78‑1 (MQ=255)
                                                                                    ttGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGt                                                                    >  2:1205550/1‑93 (MQ=255)
                                                                                    ttGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGt                                                                    <  1:1205550/93‑1 (MQ=255)
                                                                                     tGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACa                                                                                                          >  2:498466/1‑54 (MQ=255)
                                                                                     tGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACa                                                                                                          <  1:498466/54‑1 (MQ=255)
                                                                                     tGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGAc                                                                                                       >  2:48500/1‑57 (MQ=255)
                                                                                     tGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGAc                                                                                                       <  1:48500/57‑1 (MQ=255)
                                                                                     tGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCAc                               <  1:743685/129‑1 (MQ=255)
                                                                                     tGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCAc                               >  2:743685/1‑129 (MQ=255)
                                                                                     tGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCACGGTCTTTCAc                     >  2:428743/1‑139 (MQ=255)
                                                                                     tGTTATATGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCACGGTCTTTCAc                     >  2:920269/1‑139 (MQ=255)
                                                                                          tatGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCg                                                          >  1:1021764/1‑97 (MQ=255)
                                                                                          tatGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCg                                                          <  2:1021764/97‑1 (MQ=255)
                                                                                            tGAATAAAAATCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCACGGTCTTTCACCTCTGGc              >  2:1590324/1‑139 (MQ=255)
                                                                                                 aaaaaTCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCACGGTCTTTCACCTCTGGCAtttt         >  1:1164282/1‑139 (MQ=255)
                                                                                                 aaaaaTCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCACGGTCTTTCACCTCTGGCAtttt         <  1:1590324/139‑1 (MQ=255)
                                                                                                   aaaTCCCCTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCACGGTCTTTCACCTCTGGCATTTTCa       <  2:463315/139‑1 (MQ=255)
                                                                                                        cccTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGctc                                                                                  >  2:204965/1‑59 (MQ=255)
                                                                                                        cccTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGctc                                                                                  <  1:204965/59‑1 (MQ=255)
                                                                                                        cccTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAacac                                                                         >  1:1014502/1‑68 (MQ=255)
                                                                                                        cccTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAacac                                                                         <  2:1014502/68‑1 (MQ=255)
                                                                                                        cccTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCACGGTCTTTCACCTCTGGCATTTTCAGCGCa  >  2:1546260/1‑139 (MQ=255)
                                                                                                        cccTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCACGGTCTTTCACCTCTGGCATTTTCAGCGCa  >  1:831839/1‑139 (MQ=255)
                                                                                                        cccTCTCCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCACGGTCTTTCACCTCTGGCATTTTCAGCGCa  <  2:831839/139‑1 (MQ=255)
                                                                                                           tctcCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATc                                                               <  1:675177/75‑1 (MQ=255)
                                                                                                           tctcCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATc                                                               >  2:675177/1‑75 (MQ=255)
                                                                                                            ctcCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTacaaca                                                                          <  1:1628733/63‑1 (MQ=255)
                                                                                                            ctcCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTacaaca                                                                          >  2:1628733/1‑63 (MQ=255)
                                                                                                             tcCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCACGGTCTTTCACCTCTGGCATTTTCAgcgc   >  1:35538/1‑133 (MQ=255)
                                                                                                             tcCGGTAAGGGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCACGGTCTTTCACCTCTGGCATTTTCAgcgc   <  2:35538/133‑1 (MQ=255)
                                                                                                                      |                                                                                                                            
CATAAACAGGGAGTGTCGCTTTTGCTGATAACAAATTATTTCCCATAACAATTCCTTAAATATAAATATGGCAAGCTATATGTTTTGTTATATGAATAAAAATCCCCTCTCCGGTAAGAGAAGGGATTAAGGGTTTACAGACTTCTGGAAGGTTGCGCAGCTCTTACAACACGCGGTTGATCTTCCGCAGCGTCTTCCAGCGCACTTAAATCACGGTCTTTCACCTCTGGCATTTTCAGCGCA  >  NC_000913/1630868‑1631110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: