Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,655,057	T→C	T29A (ACG→GCG)	rspA ←	bifunctional D‑altronate/D‑mannonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,655,057	0	T	C	100.0%	209.6 / NA	58	T29A (ACG→GCG)	rspA	bifunctional D‑altronate/D‑mannonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/27);  total (31/27)

ACCTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAAC  >  NC_000913/1654923‑1655188                                                                                                                                       |                                                                                                                                    aCCTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATa                                                                                                                                 <  2:365987/139‑1 (MQ=255)   cTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATAcc                                                                                                                               <  1:302464/139‑1 (MQ=255)   cTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATAcc                                                                                                                               >  2:700630/1‑139 (MQ=255)      tataGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATAcc                                                                                                                               <  1:594206/136‑1 (MQ=255)      tataGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATAcc                                                                                                                               >  2:594206/1‑136 (MQ=255)          gAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGTTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCa                                                                                                                        <  1:700630/139‑1 (MQ=255)               aCTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAgtggt                                                                                                                   >  2:126399/1‑139 (MQ=255)                                     gTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTa                                                                                             <  1:126399/139‑1 (MQ=255)                                      tGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTAc                                                                                            >  2:452903/1‑139 (MQ=255)                                      tGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTAc                                                                                            >  1:31043/1‑139 (MQ=255)                                             cgcgACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCgg                                                                                     >  2:1665388/1‑139 (MQ=255)                                             cgcgACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCg                                                                                      >  2:413431/1‑138 (MQ=255)                                             cgcgACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCg                                                                                      <  1:413431/138‑1 (MQ=255)                                              gcgACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGa                                                                                    <  1:869399/139‑1 (MQ=255)                                                gACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATAcc                                                                                                                               <  1:930217/94‑1 (MQ=255)                                                gACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATAcc                                                                                                                               >  2:930217/1‑94 (MQ=255)                                                 aCCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAg                                                                                 <  2:238182/139‑1 (MQ=255)                                                     aTAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAgg                                                                                <  2:1453979/136‑1 (MQ=255)                                                     aTAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAgg                                                                                >  1:1453979/1‑136 (MQ=255)                                                            gCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                              >  2:598911/1‑115 (MQ=255)                                                            gCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                              <  1:598911/115‑1 (MQ=255)                                                            gCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGcc                                                                                         >  1:345977/1‑120 (MQ=255)                                                            gCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGcc                                                                                         <  2:345977/120‑1 (MQ=255)                                                            gCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGT‑GTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACt                                                                     >  1:1417656/1‑139 (MQ=255)                                                                    aGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCt                                                              >  1:57451/1‑139 (MQ=255)                                                                        gATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTAc                                                          <  1:1665388/139‑1 (MQ=255)                                                                         aTCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACg                                                         >  2:784879/1‑139 (MQ=255)                                                                          tCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGa                                                                                                     <  1:861265/94‑1 (MQ=255)                                                                          tCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGa                                                                                                     >  2:861265/1‑94 (MQ=255)                                                                               cAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTc                                                   >  1:1459736/1‑139 (MQ=255)                                                                                aaaTAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCa                                                  >  2:547321/1‑139 (MQ=255)                                                                                aaaTAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCa                                                  >  1:1586981/1‑139 (MQ=255)                                                                                     agagGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGt                                                                               >  1:416403/1‑105 (MQ=255)                                                                                     agagGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGt                                                                               <  2:416403/105‑1 (MQ=255)                                                                                         gCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTa                                                           <  1:547027/121‑1 (MQ=255)                                                                                         gCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTa                                                           >  2:547027/1‑121 (MQ=255)                                                                                         gCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGAtt                                         <  1:465040/139‑1 (MQ=255)                                                                                           cACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTcc                                       >  1:847611/1‑139 (MQ=255)                                                                                            aCGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACg                                                         >  2:1034976/1‑120 (MQ=255)                                                                                            aCGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACg                                                         <  1:1034976/120‑1 (MQ=255)                                                                                                   gCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCg                               >  1:1088821/1‑139 (MQ=255)                                                                                                    cTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGc                              <  2:847611/139‑1 (MQ=255)                                                                                                        cGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGt                          >  2:285764/1‑139 (MQ=255)                                                                                                        cGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGt                          >  1:1218633/1‑139 (MQ=255)                                                                                                             aTTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGa                     <  2:1586981/139‑1 (MQ=255)                                                                                                                     tGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGt                       <  2:331640/129‑1 (MQ=255)                                                                                                                     tGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGt                       >  1:331640/1‑129 (MQ=255)                                                                                                                     tGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGaa             <  2:1218633/139‑1 (MQ=255)                                                                                                                      ggCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCa                                  >  2:1156971/1‑117 (MQ=255)                                                                                                                      ggCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCa                                  <  1:1156971/117‑1 (MQ=255)                                                                                                                           ccccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCAACGCTTGTCGTGATGCATGAAATCTAc       >  2:331480/1‑139 (MQ=255)                                                                                                                             ccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGc     >  2:353821/1‑139 (MQ=255)                                                                                                                               aaGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTGCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCaa   <  2:1470176/139‑1 (MQ=255)                                                                                                                                aGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAAc  <  2:1088821/139‑1 (MQ=255)                                                                                                                                aGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAAc  >  1:196856/1‑139 (MQ=255)                                                                                                                                aGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAAc  <  1:353821/139‑1 (MQ=255)                                                                                                                                  gCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGc                                   <  1:971066/104‑1 (MQ=255)                                                                                                                                  gCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGc                                   >  2:971066/1‑104 (MQ=255)                                                                                                                                       |                                                                                                                                    ACCTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAAC  >  NC_000913/1654923‑1655188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑