Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	169,645	A→G	Y721C (TAC→TGC)	fhuA →	ferrichrome outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	169,645	0	A	G	100.0%	177.6 / NA	49	Y721C (TAC→TGC)	fhuA	ferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/24);  total (25/24)

GATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGG  >  NC_000913/169515‑169772                                                                                                                                   |                                                                                                                                gATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCa                                                                                                                         <  1:1595146/139‑1 (MQ=255)     cGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAgctg                                                                                                                     >  2:1432096/1‑139 (MQ=255)     cGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAgctg                                                                                                                     >  1:1610152/1‑139 (MQ=255)       gCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAgc                                                                                                                       >  1:1052370/1‑135 (MQ=255)       gCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAgc                                                                                                                       <  2:1052370/135‑1 (MQ=255)          aaCTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTa                                                                                                                <  2:159609/139‑1 (MQ=255)                         ggAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCACCAGCTGCTTTAACACTTATGgctgct                                                                                                 >  2:235320/1‑139 (MQ=255)                                    ggTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCa                                                                                      <  1:781688/139‑1 (MQ=255)                                                gTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTc                                                                          >  1:1652115/1‑139 (MQ=255)                                                gTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTc                                                                          >  2:588664/1‑139 (MQ=255)                                                     tACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTc                                                                                                                             <  1:677830/83‑1 (MQ=255)                                                     tACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTc                                                                                                                             >  2:677830/1‑83 (MQ=255)                                                         ttATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCACCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTgcaacc                                                                 <  1:235320/139‑1 (MQ=255)                                                                cTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCt                                                          <  2:1610152/139‑1 (MQ=255)                                                                 tGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCtt                                                         <  2:1652115/139‑1 (MQ=255)                                                                  ggCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTgggg                                                                                          <  2:880419/105‑1 (MQ=255)                                                                  ggCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTgggg                                                                                          >  1:880419/1‑105 (MQ=255)                                                                   gcgcGAGTCGGCATGGCTGGCTCCAACGTGGCGCCGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTa                                                                                                                >  1:1227592/1‑82 (MQ=255)                                                                   gcgcGAGTCGGCATGGCTGGCTCCAACGTGGCGCCGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTa                                                                                                                <  2:1227592/82‑1 (MQ=255)                                                                    cgcgAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCg                                                      >  2:988376/1‑139 (MQ=255)                                                                    cgcgAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCg                                                      >  2:265181/1‑139 (MQ=255)                                                                          tCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTgggg                                                                                          >  1:75820/1‑97 (MQ=255)                                                                          tCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTgggg                                                                                          <  2:75820/97‑1 (MQ=255)                                                                              cATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAAttt                                            <  2:138842/139‑1 (MQ=255)                                                                                   ctggctCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCtt                                       >  1:1544273/1‑139 (MQ=255)                                                                                         ccAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTgggg                                 <  2:1544273/139‑1 (MQ=255)                                                                                            aCGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAg                                                                                     >  2:392965/1‑84 (MQ=255)                                                                                            aCGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAg                                                                                     <  1:392965/84‑1 (MQ=255)                                                                                                  cgcTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTgggg                                                                                          <  2:644324/73‑1 (MQ=255)                                                                                                  cgcTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTgggg                                                                                          >  1:644324/1‑73 (MQ=255)                                                                                                       cATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTg                   >  2:808688/1‑139 (MQ=255)                                                                                                         tGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGcc                 <  2:906455/139‑1 (MQ=255)                                                                                                           ttAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCa               <  1:988376/139‑1 (MQ=255)                                                                                                            tAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGc                                >  1:1337461/1‑121 (MQ=255)                                                                                                            tAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGc                                <  2:1337461/121‑1 (MQ=255)                                                                                                              acaacCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCAttt            <  1:588664/139‑1 (MQ=255)                                                                                                                 acCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTcac         >  1:1023036/1‑139 (MQ=255)                                                                                                                 acCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTcac         >  1:1178076/1‑139 (MQ=255)                                                                                                                   cTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACaa       >  1:1652094/1‑139 (MQ=255)                                                                                                                     gTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTg                                                                                             >  2:1322243/1‑51 (MQ=255)                                                                                                                     gTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTg                                                                                             <  1:1322243/51‑1 (MQ=255)                                                                                                                     gTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGcc                 <  2:1329786/127‑1 (MQ=255)                                                                                                                     gTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGcc                 >  1:1329786/1‑127 (MQ=255)                                                                                                                     gTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGt     >  2:389465/1‑139 (MQ=255)                                                                                                                      ttCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTgc                                                                     >  2:1493809/1‑74 (MQ=255)                                                                                                                      ttCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTgc                                                                     <  1:1493809/74‑1 (MQ=255)                                                                                                                        cGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTgg  <  2:1023036/139‑1 (MQ=255)                                                                                                                            cGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGtt    <  1:688226/133‑1 (MQ=255)                                                                                                                            cGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGtt    >  2:688226/1‑133 (MQ=255)                                                                                                                                   |                                                                                                                                GATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGG  >  NC_000913/169515‑169772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑