Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,841,897 G→A *136* (TAG→TAA
T80I (ACT→ATT) 
nudG →
ynjH ←
CTP pyrophosphohydrolase, also hydrolyzes 2‑hydroxy‑dATP, 8‑hydroxy‑dGTP, 5‑hydroxy‑CTP, dCTP and 5‑methyl‑dCTP
DUF1496 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,841,8970GA100.0% 124.4 / NA 39*136* (TAG→TAA
T80I (ACT→ATT) 
nudG
ynjH
CTP pyrophosphohydrolase, also hydrolyzes 2‑hydroxy‑dATP, 8‑hydroxy‑dGTP, 5‑hydroxy‑CTP, dCTP and 5‑methyl‑dCTP
DUF1496 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/17);  total (22/17)

GCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAAC  >  NC_000913/1841772‑1842029
                                                                                                                             |                                                                                                                                    
gCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAggg                                                                                                                             <  1:580759/135‑1 (MQ=255)
gCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAggg                                                                                                                             >  2:580759/1‑135 (MQ=255)
  acaTGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGt                                                                                                                       >  1:345696/1‑139 (MQ=255)
    aTGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtca                                                                                                                     <  1:1416558/139‑1 (MQ=255)
                 gcTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGACCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCAtt                                                                                                        >  2:1667385/1‑139 (MQ=255)
                          gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGt                                                                                                >  2:548008/1‑138 (MQ=255)
                          gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGt                                                                                                <  1:548008/138‑1 (MQ=255)
                               cACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTaa                                                                                                                                >  2:843754/1‑101 (MQ=255)
                               cACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTaa                                                                                                                                <  1:843754/101‑1 (MQ=255)
                               cACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCt                                                                                          >  1:1582868/1‑139 (MQ=255)
                                                     tCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCg                                                                          <  2:984146/133‑1 (MQ=255)
                                                     tCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCg                                                                          >  1:984146/1‑133 (MQ=255)
                                                       cGCTGACCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCa                                                                  <  1:1667385/139‑1 (MQ=255)
                                                          tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtcat                                                                                                                    <  2:336271/86‑1 (MQ=255)
                                                          tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtcat                                                                                                                    >  1:336271/1‑86 (MQ=255)
                                                          tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGaa                                                               <  2:1344067/139‑1 (MQ=255)
                                                          tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGaa                                                               <  2:190503/139‑1 (MQ=255)
                                                                 tgctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtcat                                                                                                                    >  1:1291440/1‑79 (MQ=255)
                                                                 tgctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtcat                                                                                                                    <  2:1291440/79‑1 (MQ=255)
                                                                                      ggCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                         >  1:1636310/1‑85 (MQ=255)
                                                                                      ggCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                         <  2:1636310/85‑1 (MQ=255)
                                                                                      ggCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACt                                   >  2:1203958/1‑139 (MQ=255)
                                                                                          tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCGTCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGt                               >  2:1304528/1‑139 (MQ=255)
                                                                                          tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGaat                                                              >  2:1019827/1‑108 (MQ=255)
                                                                                          tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGaat                                                              <  1:1019827/108‑1 (MQ=255)
                                                                                          tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGt                               >  2:403880/1‑139 (MQ=255)
                                                                                           ttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCt                                                                     <  2:1233474/100‑1 (MQ=255)
                                                                                           ttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCt                                                                     >  1:1233474/1‑100 (MQ=255)
                                                                                           ttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTa                              <  1:403880/139‑1 (MQ=255)
                                                                                            tATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATGGCAGCAGTATTCCTTCGGCTTTAATTACCGCGCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTAc                             >  1:1087288/1‑139 (MQ=255)
                                                                                                  tttACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCt                       >  1:1342878/1‑139 (MQ=255)
                                                                                                    tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCt                                                                     <  1:704837/91‑1 (MQ=255)
                                                                                                    tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCt                                                                     >  2:704837/1‑91 (MQ=255)
                                                                                                    tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgc                     >  2:924228/1‑139 (MQ=255)
                                                                                                     aCGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgcg                    >  1:1345220/1‑139 (MQ=255)
                                                                                                         gccgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGa                <  1:924228/139‑1 (MQ=255)
                                                                                                           cgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTg                                                                                                       <  2:348796/50‑1 (MQ=255)
                                                                                                           cgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTg                                                                                                       >  1:348796/1‑50 (MQ=255)
                                                                                                                       ggATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaac  >  1:640479/1‑139 (MQ=255)
                                                                                                                             |                                                                                                                                    
GCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAAC  >  NC_000913/1841772‑1842029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: