| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,842,910 | G→A | A180A (GCG→GCA) | gdhA → | glutamate dehydrogenase, NADP‑specific |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,842,910 | 0 | G | A | 95.0% | 114.2 / ‑1.5 | 40 | A180A (GCG→GCA) | gdhA | glutamate dehydrogenase, NADP‑specific |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (17/21); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
AAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCT > NC_000913/1842777‑1843038 | aaGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGgat < 2:1010998/139‑1 (MQ=255) cGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAg < 2:760517/139‑1 (MQ=255) aGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGctc < 1:1508698/139‑1 (MQ=255) aGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGctc < 2:55940/139‑1 (MQ=255) aGGTGAAGTGATGCGTTTTTGCCAGGCGCTGAAGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGctc < 1:512558/139‑1 (MQ=255) aTGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATa > 2:1153684/1‑139 (MQ=255) tttttGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGcc < 2:732102/139‑1 (MQ=255) tttttGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGcc > 1:1595426/1‑139 (MQ=255) tGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCg < 2:1595426/139‑1 (MQ=255) gatgaCTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAg > 2:971196/1‑106 (MQ=255) gatgaCTGAACTGTATCGCCACCTGGGCGCGGATACAGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAGAAAg < 1:971196/106‑1 (MQ=255) tGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGctc < 2:878580/98‑1 (MQ=255) tGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGctc > 1:878580/1‑98 (MQ=255) tCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGc < 2:1596391/92‑1 (MQ=255) tCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGc > 1:1596391/1‑92 (MQ=255) aCCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTggcgg < 2:641455/139‑1 (MQ=255) aCCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTggcgg < 1:1153684/139‑1 (MQ=255) tGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGaaaa < 1:141374/81‑1 (MQ=255) tGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGaaaa > 2:141374/1‑81 (MQ=255) tGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAg < 1:1566851/83‑1 (MQ=255) tGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAg > 2:1566851/1‑83 (MQ=255) cgcgGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCtt > 1:1287605/1‑139 (MQ=255) gATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTc > 2:1230597/1‑139 (MQ=255) tCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGga > 2:587464/1‑53 (MQ=255) tCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGga < 1:587464/53‑1 (MQ=255) atCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTggcgg > 1:1206122/1‑104 (MQ=255) atCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTggcgg < 2:1206122/104‑1 (MQ=255) gggTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGt < 2:1287605/139‑1 (MQ=255) ggTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGa > 2:1453319/1‑117 (MQ=255) ggTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGa < 1:1453319/117‑1 (MQ=255) gTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGt > 1:504807/1‑78 (MQ=255) gTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGt < 2:504807/78‑1 (MQ=255) gtggtCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCAtt > 1:1099175/1‑88 (MQ=255) gtggtCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCAtt < 2:1099175/88‑1 (MQ=255) tggtCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTAt > 2:212720/1‑136 (MQ=255) tggtCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTAt < 1:212720/136‑1 (MQ=255) tggtCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTc > 2:41753/1‑139 (MQ=255) gCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCt > 2:265352/1‑139 (MQ=255) aTGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTc < 2:618311/48‑1 (MQ=255) aTGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTc > 1:618311/1‑48 (MQ=255) | AAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCT > NC_000913/1842777‑1843038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |