Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,892,402	A→G	S278S (AGT→AGC)	yoaA ←	putative ATP‑dependent helicase, DinG family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,892,402	0	A	G	100.0%	199.6 / NA	56	S278S (AGT→AGC)	yoaA	putative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/28);  total (28/28)

CGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACT  >  NC_000913/1892270‑1892529                                                                                                                                     |                                                                                                                                cGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTgg                                                                                                                           >  1:1211480/1‑139 (MQ=255)             aCAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGct                                                                                                                               <  1:1179488/123‑1 (MQ=255)                aaGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                                            <  1:138912/139‑1 (MQ=255)                      cAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGct                                                                                                                               <  2:54049/114‑1 (MQ=255)                      cAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGct                                                                                                                               >  1:54049/1‑114 (MQ=255)                             tCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGt                                                                                                   >  1:1266448/1‑135 (MQ=255)                             tCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGt                                                                                                   <  2:1266448/135‑1 (MQ=255)                             tCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAAct                                                                                               >  1:783476/1‑139 (MQ=255)                             tCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAAct                                                                                               <  2:783476/139‑1 (MQ=255)                             tCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAAct                                                                                               >  2:1500865/1‑139 (MQ=255)                                    gCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgt                                                                                        <  2:1393107/139‑1 (MQ=255)                                    gCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgt                                                                                        <  1:1374669/139‑1 (MQ=255)                                    gCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgt                                                                                        <  1:1413636/139‑1 (MQ=255)                                     cAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgtg                                                                                       >  1:1540541/1‑139 (MQ=255)                                       gTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                           <  1:1231032/117‑1 (MQ=255)                                       gTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                           >  2:1231032/1‑117 (MQ=255)                                       gTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTc                                                                                     >  2:314703/1‑139 (MQ=255)                                               gCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAAtt                                                                             <  1:314703/139‑1 (MQ=255)                                                cccGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgtg                                                                                       <  1:1311169/128‑1 (MQ=255)                                                cccGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgtg                                                                                       >  2:1311169/1‑128 (MQ=255)                                                cccGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTc                                                                            >  2:597048/1‑139 (MQ=255)                                                  cGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCgg                                                                          <  2:1211480/139‑1 (MQ=255)                                                        aTTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGt                                                                    <  1:597048/139‑1 (MQ=255)                                                                           gCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTggg                                                                                         >  1:1460228/1‑99 (MQ=255)                                                                           gCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTggg                                                                                         <  2:1460228/99‑1 (MQ=255)                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGct                                                                                                                               >  1:633560/1‑60 (MQ=255)                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACt                                                                                                        >  1:351023/1‑83 (MQ=255)                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACt                                                                                                        <  2:351023/83‑1 (MQ=255)                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTggg                                                                                         <  1:315038/98‑1 (MQ=255)                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTggg                                                                                         >  2:315038/1‑98 (MQ=255)                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgt                                                                                        <  1:415620/99‑1 (MQ=255)                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgt                                                                                        >  2:415620/1‑99 (MQ=255)                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCtttt                                                >  2:68125/1‑139 (MQ=255)                                                                              cACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCgg                                                                     >  1:945090/1‑116 (MQ=255)                                                                              cACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCgg                                                                     <  2:945090/116‑1 (MQ=255)                                                                              cACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGc                                              >  1:1637113/1‑139 (MQ=255)                                                                               aCGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                           <  2:1187527/77‑1 (MQ=255)                                                                               aCGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGcc                                             <  2:1637113/139‑1 (MQ=255)                                                                               aCGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCCCTGGGCAAGACGATCAgcgc                                                                                                           >  1:1187527/1‑77 (MQ=255)                                                                                        aCCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTTGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGc                                    >  1:384683/1‑139 (MQ=255)                                                                                         ccGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGa                                                              >  2:940492/1‑112 (MQ=255)                                                                                         ccGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGa                                                              <  1:940492/112‑1 (MQ=255)                                                                                           gcgATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                                            >  1:661144/1‑64 (MQ=255)                                                                                           gcgATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                                            <  2:661144/64‑1 (MQ=255)                                                                                               tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGct                                                                                                                               >  1:1460562/1‑41 (MQ=255)                                                                                                       cTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGgag                     >  1:1040602/1‑139 (MQ=255)                                                                                                         cACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCg                                                                           >  2:1459191/1‑83 (MQ=255)                                                                                                         cACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCg                                                                           <  1:1459191/83‑1 (MQ=255)                                                                                                                   cAGACGAAAATCCTGCGCGCTCTGGGCAAGGCGATCAGCTCAGTTTTGTAACTGCTGAGTGTCTTTTAATTCGGTGTGGTAGTCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCa         <  1:646956/139‑1 (MQ=255)                                                                                                                      aCGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCg                                                                           <  2:182060/70‑1 (MQ=255)                                                                                                                      aCGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCg                                                                           >  1:182060/1‑70 (MQ=255)                                                                                                                      aCGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGAACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCaaaa      >  2:646956/1‑139 (MQ=255)                                                                                                                        gAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGa                                                        >  2:488687/1‑87 (MQ=255)                                                                                                                        gAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGa                                                        <  1:488687/87‑1 (MQ=255)                                                                                                                        gAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATa    <  1:1500865/139‑1 (MQ=255)                                                                                                                          aaaTCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATAct  <  2:1540541/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                CGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACT  >  NC_000913/1892270‑1892529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑