Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,926,096	G→A	*219* (TAG→TAA)	yobB →	C‑N hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,926,096	0	G	A	100.0%	282.2 / NA	89	*219* (TAG→TAA)	yobB	C‑N hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/43);  total (46/43)

CCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGT  >  NC_000913/1925969‑1926229                                                                                                                                |                                                                                                                                      cccGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGc                                                                                                                            >  2:491889/1‑139 (MQ=255)   cGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCt                                                                                                                          <  2:1418443/139‑1 (MQ=255)             gTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGcc                                                                                                                <  2:563611/139‑1 (MQ=255)              tGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGTGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           <  1:25678/127‑1 (MQ=255)              tGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGTGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           >  2:25678/1‑127 (MQ=255)                cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGcc                                                                                                                <  2:1298352/136‑1 (MQ=255)                cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGcc                                                                                                                >  1:1298352/1‑136 (MQ=255)                     tGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                  >  1:59644/1‑129 (MQ=255)                     tGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                  <  2:59644/129‑1 (MQ=255)                      gggATGAATGTGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                       <  1:280983/139‑1 (MQ=255)                      gggATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                       <  2:1394028/139‑1 (MQ=255)                          tGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTa                                                                                                   >  2:1354660/1‑139 (MQ=255)                             atatGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCg                                                                                                >  2:1565895/1‑139 (MQ=255)                               atGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGAt                                                                                              >  2:606003/1‑139 (MQ=255)                                  gtcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                       <  2:1181335/111‑1 (MQ=255)                                  gtcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                       <  1:218329/111‑1 (MQ=255)                                  gtcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                       >  2:218329/1‑111 (MQ=255)                                  gtcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                       >  1:1181335/1‑111 (MQ=255)                                    cgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGa                                                                                         <  2:521030/139‑1 (MQ=255)                                      tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCttttt                                                                                                                                >  2:1387913/1‑98 (MQ=255)                                      tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCttttt                                                                                                                                <  1:1387913/98‑1 (MQ=255)                                      tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           <  1:1127491/103‑1 (MQ=255)                                      tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           >  2:1127491/1‑103 (MQ=255)                                      tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                  >  2:1094724/1‑112 (MQ=255)                                      tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                  <  1:1035846/128‑1 (MQ=255)                                      tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                  >  2:1035846/1‑128 (MQ=255)                                      tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGaaa                                                                                       >  2:427182/1‑139 (MQ=255)                                      tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTGCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTTGAGCATg                                                                                                                  <  1:1094724/112‑1 (MQ=255)                                          aTCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                       >  1:1024047/1‑103 (MQ=255)                                          aTCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                       <  2:1024047/103‑1 (MQ=255)                                          aTCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTg                                                                                   <  2:1246667/139‑1 (MQ=255)                                          aTCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTg                                                                                   >  2:292033/1‑139 (MQ=255)                                                cgcgCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGttt                                                                             >  1:1270042/1‑139 (MQ=255)                                                 gcgcCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTg                                                                            <  1:606003/139‑1 (MQ=255)                                                 gcgcCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTg                                                                            <  1:292033/139‑1 (MQ=255)                                                  cgcCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           >  1:1644480/1‑91 (MQ=255)                                                  cgcCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           <  2:1644480/91‑1 (MQ=255)                                                   gcCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCa                                                                          <  2:1270042/139‑1 (MQ=255)                                                      gATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCaggg                                                                       >  2:895921/1‑139 (MQ=255)                                                        tCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggag                                                                     <  1:427182/139‑1 (MQ=255)                                                             gTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACa                                                                                           <  1:1395279/112‑1 (MQ=255)                                                             gTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACa                                                                                           >  2:1395279/1‑112 (MQ=255)                                                             gTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGc                                                                           <  1:27659/128‑1 (MQ=255)                                                             gTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGc                                                                           >  2:27659/1‑128 (MQ=255)                                                                  ttattGTGAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           >  1:1407213/1‑75 (MQ=255)                                                                  ttattGTGAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           <  2:1407213/75‑1 (MQ=255)                                                                   tattGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCtttt                                                                                                                                 <  2:569848/68‑1 (MQ=255)                                                                   tattGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCtttt                                                                                                                                 >  1:569848/1‑68 (MQ=255)                                                                   tattGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTgag                                                          <  1:491889/139‑1 (MQ=255)                                                                        ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                       <  2:1193792/73‑1 (MQ=255)                                                                        ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                       >  1:1193792/1‑73 (MQ=255)                                                                          aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGTGATATCATTCCATTACGCTAAGCtttt                                                                                                                                 >  2:1311279/1‑61 (MQ=255)                                                                          aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGTGATATCATTCCATTACGCTAAGCtttt                                                                                                                                 <  1:1311279/61‑1 (MQ=255)                                                                          aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                             <  2:1186135/81‑1 (MQ=255)                                                                          aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                             >  1:1186135/1‑81 (MQ=255)                                                                           gTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGAt                                                        <  2:951778/133‑1 (MQ=255)                                                                           gTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGAt                                                        >  1:951778/1‑133 (MQ=255)                                                                               gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                       >  2:1240134/1‑66 (MQ=255)                                                                               gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                       <  1:1240134/66‑1 (MQ=255)                                                                               gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                  <  1:87654/87‑1 (MQ=255)                                                                               gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                  >  2:87654/1‑87 (MQ=255)                                                                                tCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTg                                             >  2:416533/1‑139 (MQ=255)                                                                                              aGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCAtt                                                                                                    >  2:1195985/1‑70 (MQ=255)                                                                                              aGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCAtt                                                                                                    <  1:1195985/70‑1 (MQ=255)                                                                                              aGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                  <  2:1368448/72‑1 (MQ=255)                                                                                              aGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                  >  1:1368448/1‑72 (MQ=255)                                                                                              aGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGa                               >  1:386516/1‑139 (MQ=255)                                                                                               gggTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTCGAGATTGCCTCTGTTGATGTCATTGACGGaa                              <  2:386516/139‑1 (MQ=255)                                                                                                ggTTGGCAAGGCGATATCATTCCATTACGCTAAGCttttt                                                                                                                                >  2:205397/1‑40 (MQ=255)                                                                                                ggTTGGCAAGGCGATATCATTCCATTACGCTAAGCttttt                                                                                                                                <  1:205397/40‑1 (MQ=255)                                                                                                   tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGTAGCATg                                                                                                                  <  1:662901/51‑1 (MQ=255)                                                                                                   tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                  >  2:662901/1‑51 (MQ=255)                                                                                                       aaGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTc                      <  1:944704/139‑1 (MQ=255)                                                                                                          gCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           >  2:481649/1‑35 (MQ=255)                                                                                                          gCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           <  1:481649/35‑1 (MQ=255)                                                                                                          gCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAc                   >  2:686281/1‑139 (MQ=255)                                                                                                               atCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                             <  2:1002867/44‑1 (MQ=255)                                                                                                               aacATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                             >  1:1002867/3‑44 (MQ=255)                                                                                                                 cATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGa            <  2:42050/139‑1 (MQ=255)                                                                                                                    tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGcc         >  2:512935/1‑139 (MQ=255)                                                                                                                     ccATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCa        >  2:1393593/1‑139 (MQ=255)                                                                                                                        ttACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                       >  1:702801/1‑41 (MQ=255)                                                                                                                        ttACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                       <  2:702801/41‑1 (MQ=255)                                                                                                                        ttACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCt     >  2:1212784/1‑139 (MQ=255)                                                                                                                          aCGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTgg   >  2:971390/1‑139 (MQ=255)                                                                                                                           cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGAt                                                        <  2:1502934/85‑1 (MQ=255)                                                                                                                           cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGAt                                                        >  1:1502934/1‑85 (MQ=255)                                                                                                                           cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGt  >  1:1471684/1‑139 (MQ=255)                                                                                                                             cTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATc                                                                                                 >  1:1581237/1‑42 (MQ=255)                                                                                                                                |                                                                                                                                      CCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGT  >  NC_000913/1925969‑1926229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑